Testosterone induces a rapid stimulation of endocytosis, amino acid and hexose transport in mouse kidney cortex

Koenig, Harold; Goldstone, Alfred; Lu, Chung Y.

doi:10.1016/0006-291x(82)91116-0

Cited by 38 publications

(5 citation statements)

References 9 publications

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“…Under pharmacological stimulation by testosterone, several genes are over-expressed in the kidney [4]. In addition, endocytosis, amino acid and glucose transport are induced in the mouse kidney cortex [11]. Our study shows that ODC induction is independent of any effect of testosterone on amino acid transport since tubules were permeabilized.…”

Section: Discussionmentioning

confidence: 72%

Ornithine metabolism along the female mouse nephron: localization of ornithine decarboxylase and ornithine aminotransferase

Levillain

Diaz

Reymond

et al. 2000

Pflugers Arch - Eur J Physiol

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The fate of ornithine in the nephron of the female OF-1 Swiss mouse remains unknown. The aim of the present study was to identify the nephron segments containing the key enzymes involved in ornithine metabolism: ornithine decarboxylase (ODC) and ornithine aminotransferase (OAT). Viable tubules isolated by microdissection were incubated with [1-14C]ornithine to study the oxidative pathway. Other tubules were permeabilized to measure the ODC activity. Ornithine was decarboxylated in all intact tubules. Gabaculine, a suicide inhibitor of OAT, and rotenone sharply decreased the production of 14CO2 from [1-14C]ornithine. No ODC activity was found in permeabilized tubules isolated from untreated mice. Testosterone increased ODC activity in the proximal tubule substantially and to a minor extent in other nephron segments. In situ hybridization showed ODC messenger ribonucleic acid (mRNA) to be absent in kidneys of untreated females but abundant in the cortex and the outer stripe of the outer medulla of testosterone-treated female mice. The whole proximal tubule contained a great density of silver grains corresponding to ODC mRNA. In conclusion, no basal ODC activity was found in the nephron of female mice. The testosterone-inducible ODC is localized mainly in the proximal tubule, but is also present in distal tubules and collecting ducts. OAT is distributed along the whole nephron, but its activity is higher in proximal tubules than in distal tubules.

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Section: Discussionmentioning

confidence: 72%

Ornithine metabolism along the female mouse nephron: localization of ornithine decarboxylase and ornithine aminotransferase

Levillain

Diaz

Reymond

et al. 2000

Pflugers Arch - Eur J Physiol

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“…Thus, both processes start by the binding of cations to the membrane, both are dependent on Ca2`-influx (Josefsson, 1968;Prusch & Hannafin, 1979;Goldstone etal., 1983;Lu etal., 1983), and both are sensitive to hormones (Josefsson et al, 1972;Josefsson & Johansson, 1979;Koenig et al, 1982). Netilmicin, which inhibits Cal-dependent pinocytosis in the amoeba, may have a similar effect on tubular cells.…”

Section: Discussionmentioning

confidence: 99%

Induction and inhibition of pinocytosis by aminoglycoside antibiotics

Johansson¹,

Josefsson²,

Nässberger

1984

British J Pharmacology

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“…For example, testosterone was shown to stimulate amino acid uptake in murine renal cortical slices (Koenig et al, 1982), parathyroid hormone increases amino acid excretion by inhibition of amino acid reabsorption (Scriver and Bergeron, 1974), and dexamethasone stimulates taurine transport in flounder renal tubules (King et al, 1982). In own experiments (Fleck, 1992a), repeated administration of triiodothyronine (T3) or dexamethasone caused significant changes of endogenous amino acid plasma concentrations in young (10 days old) and adult rats (2 months old).…”

Section: Introductionmentioning

confidence: 99%

Stimulation of renal amino acid reabsorption after treatment with triiodothyronine or dexamethasone in amino acid loaded rats

1997

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Summary.In adult female anaestetized rats, the influence of triiodothyronine or dexamethasone on renal amino acid handling was investigated in leucine (20mg/100g b.wt.) or glutamine (45mg/100g b.wt.) loaded animals. Bolus injections of both amino acids were followed by temporary increase in fractional excretion of the administered amino acids as well of the endogenous amino acids which were not administered.Under load conditions (leucine and glutamine), dexamethasone treatment (60/~g/100 g b.wt. for 3 days, i.p. once daily) was followed by a stimulation of renal amino acid reabsorption. The increase in fractional amino acid excretion after amino acid load was significantly lower than in untreated rats. The effect of triiodothyronine (20/~g/100 g b.wt. for 3 days, i.p. once daily) was different in leucine and glutamine loaded animals: after leucine bolus injection a comparable stimulatory effect as shown for dexamethasone could be demonstrated, but after glutamine administration the stimulatory action of T3 was masked. T3 even increases fractional amino acid excretion in glutamine loaded rats as a sign of enhanced "house-keeping" in the renal tubular cells. These results confirm previous findings and indicate different effects of both hormones on the renal handling of amino acids.

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Testosterone induces a rapid stimulation of endocytosis, amino acid and hexose transport in mouse kidney cortex

Cited by 38 publications

References 9 publications

Ornithine metabolism along the female mouse nephron: localization of ornithine decarboxylase and ornithine aminotransferase

Ornithine metabolism along the female mouse nephron: localization of ornithine decarboxylase and ornithine aminotransferase

Induction and inhibition of pinocytosis by aminoglycoside antibiotics

Stimulation of renal amino acid reabsorption after treatment with triiodothyronine or dexamethasone in amino acid loaded rats

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