Testosterone Therapy Differently Regulates the Anti‐ and Pro‐Inflammatory Cytokines in the Plasma and Prostate of Rats Submitted to Chronic Ethanol Consumption (UChB)

Mendes, Leonardo O.; Scarano, Wellerson Rodrigo; Rochel-Maia, Sabrina S.; Fioruci-Fontaneli, Beatriz Aparecida; Chuffa, Luiz Gustavo de Almeida; Martinez, Francisco Eduardo

doi:10.1111/aji.12268

Cited by 14 publications

(9 citation statements)

References 37 publications

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“…These previous animal studies as well as the current one indicated that exogenous testosterone supplementation might not alter the cytokines production in the body. On the other hand, Mendes et al showed that the combination of ethanol consumption and testosterone treatment (testosterone cypionate 5 mg/kg every other day for 4 weeks) caused a significant increase in TNFα, but not IL-6 in rats ( 15 ). However, each compound alone did not significantly change the level of cytokines in rats ( 15 ).…”

Section: Discussionmentioning

confidence: 99%

“…On the other hand, Mendes et al showed that the combination of ethanol consumption and testosterone treatment (testosterone cypionate 5 mg/kg every other day for 4 weeks) caused a significant increase in TNFα, but not IL-6 in rats ( 15 ). However, each compound alone did not significantly change the level of cytokines in rats ( 15 ). The results of the study by Mendes et al pointed out that exogenous testosterone supplementation might aggravate the inflammatory properties of other compounds.…”

Section: Discussionmentioning

confidence: 99%

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The Effects of Testosterone Deficiency and Its Replacement on Inflammatory Markers in Rats: A Pilot Study

Chin

Ima‐Nirwana

2017

Int J Endocrinol Metab

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BackgroundTestosterone deficiency is linked to low-grade inflammation in humans, but this condition is not replicated in an animal study. The current study aims at determining the effects of testosterone deficiency and its replacement on the circulating inflammatory cytokine level in orchidectomized male rats.MethodsThree-month-old Sprague-Dawley male rats (n = 18) were randomized equally into 3 groups. Bilateral orchidectomy was performed on 2 groups. The sham group was subjected to similar surgical stress, but their testes were retained. One of the orchidectomized groups received intramuscular injection of 7 mg/kg testosterone enanthate suspended in peanut oil weekly and the other 2 groups received equivolume of peanut oil injection. After 8 weeks, the rats were sacrificed and their blood was collected for the analysis of the levels of inflammatory cytokines and testosterone.ResultsTestosterone level was significantly lower in the untreated orchidectomized group compared to the sham group. Testosterone replacement significantly increased the level of testosterone in the orchidectomized rats compared to the sham and untreated orchidectomized rats. Interleukin-1 alpha (IL-1α), interleukin-1 beta (IL-1β), and tumor necrosis factor alpha (TNFα) showed an increasing trend in orchidectomized rats, albeit not statistically significant. Interleukin-6 (IL-6) level increased significantly in the orchidectomized group compared to the sham group. Testosterone replacement at the supraphysiological dose did not alter the level of inflammatory cytokines significantly in orchidectomized rats.ConclusionsTestosterone deficiency can elicit a state of low-grade inflammation, shown by an increase in interleukin-6 level, but exogenous supraphysiological testosterone replacement does not suppress the inflammation.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

The Effects of Testosterone Deficiency and Its Replacement on Inflammatory Markers in Rats: A Pilot Study

Chin

Ima‐Nirwana

2017

Int J Endocrinol Metab

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“…Despite the large amount of data regarding prostatic changes caused by ethanol (Fioruci‐Fontanelli et al, 2015), the mechanisms by which chronic alcohol consumption modulates metabolism in the prostate remain poorly understood. The earliest studies about effects of alcohol consumption in the prostate suggested that long‐term drinking is associated with inflammation (Mendes et al, 2014), decreases in levels of retinoic acid via CYP2E1 enzyme is involved mainly in the catabolism of ethanol (Fioruci‐Fontanelli et al, 2015), increased lipids accumulation in cytoplasmatic cell (You & Arteel, 2019), increased serum estrogen resulting in hyperproliferation related with prostatic intraepithelial neoplasia (PIN). In addition, results show genotoxic effects of acetaldehyde, a product of the oxidation of ethanol, induces the activation of microsomal cytochrome P450 2E1 (CYP2E1), a process that produces reactive oxygen species, that leads to lipid peroxidation and mutagenic adducts (Deshpande et al, 2014).…”

Section: Introductionmentioning

confidence: 99%

Strength training protects against prostate injury in alcoholic rats

Teixeira

Chuffa

Mendes

et al. 2020

Journal Cellular Physiology

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Alcoholic injury can alter the hormonal signaling pathway and lead to glucose and lipid metabolism disorders. In this study, we investigated whether the strength training could exert protective effects against the alterations caused by ethanol consumption on prostatic metabolism. A UChB, ethanol‐preferring rats were used in this study. Strength training was conducted for 3 days per week for 13 weeks, rats performed jumps in water carrying a weight load strapped to their chests as part of a strength training protocol. The reduced alcohol consumption by strength training was accompanied by increased glucose, serum lipid profile, total protein levels, and reduced hormonal levels. The results of protein expression of prostatic tissues in the ethanol‐ and strength training‐treated groups indicated that “steroidal hormone receptors,” “fatty acid translocation,” and “cell regulation” were significantly different between ethanol‐ and strength training‐treated groups. Taken together, these findings show that strength training effectively ameliorated prostatic injuries in alcoholic rats at least partially by acting on lipids receptors and steroidal hormone receptors pathway, suggesting the strength training as a potential novel therapeutic strategy for treating prostate injuries caused by ethanol.

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“…The minimum detection limits were 21 pg/mL (IL‐6), 10 pg/mL (IL‐10), 5 pg/mL (TNF‐α), and 4.6 pg/mL (TGF‐β1). The protocol was standardized according to Mendes et al ().…”

Section: Methodsmentioning

confidence: 99%

“…The number of intact and degranulated mast cells was measured in five animals of each experimental group and the values were given in cell/mm 2 . Random images of 50 histological fields per experimental group (10 fields/rat as described in morphometric–stereological analysis) of were captured by digital photomicroscope Axiophot II—Zeiss using 40× objective, with each field covering an area of 0.085 mm 2 , according to Mendes et al ().…”

Section: Methodsmentioning

confidence: 99%

Modulation of inflammatory and hormonal parameters in response to testosterone therapy: Effects on the ventral prostate of adult rats

Mendes

Castilho

Pinho

et al. 2018

Cell Biology International

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Testosterone is often recommended in the treatment of several aging-related conditions. However, there are still questions about the consequences of this therapy in terms of hormonal and inflammatory parameters that are crucial for prostate homeostasis. Thus, we investigate if the testosterone therapy (TT) modulates the hormone receptors and inflammatory cytokines in the ventral prostate of adult rats. Wistar rats aging 150 days were divided into two experimental groups (n = 10/group): T: received subcutaneous injections of testosterone cypionate (5 mg/kg body weight) diluted in corn oil every other day for 4 weeks; and C: received corn oil as vehicle. Animals were euthanized at 180 days old by decapitation. Blood was collected to obtain hormone and cytokines concentrations. The ventral prostate was dissected and processed for light microscope and molecular analyses. Relative ventral prostate weight and epithelial compartment were increased after TT. The number of intact and degranulated mast cells was reduced in the T group. Plasma testosterone, DHT and intraprostatic testosterone concentrations were higher in the T group. TT leads to an increase in cell proliferation and up-regulation of AR, ERβ, PAR-4, and NRF2. Importantly, plasma concentration and tissue expression of IL-10 and TNF-α were higher after TT. In summary, these results indicate that TT can regulate inflammatory response, with impacts in cytokines and mast cell population, and modulates steroids receptors, important parameters for prostatic homeostasis.

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Testosterone Therapy Differently Regulates the Anti‐ and Pro‐Inflammatory Cytokines in the Plasma and Prostate of Rats Submitted to Chronic Ethanol Consumption (UChB)

Abstract: So, ethanol and testosterone differentially modulates the cytokines in the plasma and prostate.

Cited by 14 publications

References 37 publications

The Effects of Testosterone Deficiency and Its Replacement on Inflammatory Markers in Rats: A Pilot Study

The Effects of Testosterone Deficiency and Its Replacement on Inflammatory Markers in Rats: A Pilot Study

Strength training protects against prostate injury in alcoholic rats

Modulation of inflammatory and hormonal parameters in response to testosterone therapy: Effects on the ventral prostate of adult rats

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