Tetra-primer amplification refractory mutation system PCR (T-ARMS-PCR) rapidly identified a critical missense mutation (P236T) of bovine ACADVL gene affecting growth traits

The evaluation of 23-bp and 12-bp insertion/deletion within the PRNP gene and their effects on growth traits in healthy Chinese native cattle breeds

“…Xia'nan and Pi'nan cattle are the crossbreeding beef cattle breeds in China, instead, Qinchuan, Nanyang, Jiaxian and Ji'an cattle are Chinese indigenous meat breeds. The growth traits in unrelated Nanyang cattle were recorded at 6, 12, 18 and 24 months, including body weight (kg), body height (cm), body length (cm), heart girth (cm), hucklebone width (cm) and average daily weight gain (kg) (Pan et al 2013;Zhang et al 2015). Records of growth traits in unrelated adult Qinchuan and Jiaxian cattle were also measured at similar intervals including body weight (kg), body height (cm), body length (cm), chest girth (cm), chest width (cm), chest depth (cm), cannon circumference (cm), rump length (cm), height at hip cross (cm), hucklebone width (cm), hip width (cm) and back height (cm) .…”

Evaluation of novel SNPs and haplotypes within the <i>ATBF1</i> gene and their effects on economically important production traits in cattle

“…The result showed that the consistency of these two methods is 98.8 %, 40 % of inconsistency was caused by PCR-RFLP and 60 % of inconsistency was caused by the T-ARMS-PCR method (Li et al, 2014). Our previous study also identified that the accuracy of T-ARMS-PCR and PCR-RFLP reached 99.07 and 99.69 %, respectively, based on the sequencing result (Zhang et al, 2015a). Genetic diversity analysis found that three loci were not at Hardy-Weinberg equilibrium.…”

“…Since all cattle were adult females and each breed was fed the same nutritional diet on their respective farms, the basic linear model Y = µ + G + e was used to determine the relationship between genotypes, haplotypes and growth traits for each breed. In the formulate, Y denotes the trait data of each animal, µ the overall mean for each trait, G the effect of genotype and e the random error Zhang et al, 2015a).…”

“…This method needs to change one or two nucleotides, which are close to the SNP locus, to make this sequence recognizable using restriction enzymes and genotyping (Huang et al, 2014). For the SNP locus, which is difficult to genotype using the two methods above, T-ARMS-PCR is available Wang et al, 2014;Zhang et al, 2015a). The preferred methods are PCR-RFLP and forced PCR-RFLP because they are more accurate and mature than T-ARMS-PCR Sun et al, 2013).…”

“…The growth trait data of the Jinnan cattle, including body height, height across the hip, chest circumference, rump length and body side length, were collected from the Jinnan cattle farm. All growth trait data were measured as described by Zhang et al (2015a).…”