Tetrahedral framework nucleic acids-based delivery of microRNA-155 inhibits choroidal neovascularization by regulating the polarization of macrophages

Xin, Qin; Xiao, Lirong; Ni, Li; Hou, Chen; Li, Wenman; Li, Jiajie; Yan, Naihong; Lin, Yunfeng

doi:10.1016/j.bioactmat.2021.11.031

Cited by 89 publications

(78 citation statements)

References 64 publications

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“…This system has undergone systematic biocompatibility and safety evaluation in minipigs and has been further extended as an antigen-presenting and siRNA delivery system for immunotherapy. 36 , 37 , 138 , 139 These studies show the prospect of DNA nanostructures as delivery vehicles. However, the majority of research and applications are limited to in vitro experiments and in vivo exploration of simple delivery strategies.…”

Section: Prospects and Challenges In The Use Of Dynamic Dna Nanostruc...mentioning

confidence: 89%

Prospects and challenges of dynamic DNA nanostructures in biomedical applications

Tian

Lin

2022

Bone Res

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The physicochemical nature of DNA allows the assembly of highly predictable structures via several fabrication strategies, which have been applied to make breakthroughs in various fields. Moreover, DNA nanostructures are regarded as materials with excellent editability and biocompatibility for biomedical applications. The ongoing maintenance and release of new DNA structure design tools ease the work and make large and arbitrary DNA structures feasible for different applications. However, the nature of DNA nanostructures endows them with several stimulus-responsive mechanisms capable of responding to biomolecules, such as nucleic acids and proteins, as well as biophysical environmental parameters, such as temperature and pH. Via these mechanisms, stimulus-responsive dynamic DNA nanostructures have been applied in several biomedical settings, including basic research, active drug delivery, biosensor development, and tissue engineering. These applications have shown the versatility of dynamic DNA nanostructures, with unignorable merits that exceed those of their traditional counterparts, such as polymers and metal particles. However, there are stability, yield, exogenous DNA, and ethical considerations regarding their clinical translation. In this review, we first introduce the recent efforts and discoveries in DNA nanotechnology, highlighting the uses of dynamic DNA nanostructures in biomedical applications. Then, several dynamic DNA nanostructures are presented, and their typical biomedical applications, including their use as DNA aptamers, ion concentration/pH-sensitive DNA molecules, DNA nanostructures capable of strand displacement reactions, and protein-based dynamic DNA nanostructures, are discussed. Finally, the challenges regarding the biomedical applications of dynamic DNA nanostructures are discussed.

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Section: Prospects and Challenges In The Use Of Dynamic Dna Nanostruc...mentioning

confidence: 89%

Prospects and challenges of dynamic DNA nanostructures in biomedical applications

Tian

Lin

2022

Bone Res

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“…Masson's trichrome staining showed that the stFNA-miR group exhibited a significantly higher collagen fiber content than the control and other groups after one and two weeks. Another group, Qin et al developed microRNA-155-equipped tFNAs (T-155) and determined the effects on choroidal neovascularization (CNV) [131]. They targeted macrophages instead of targeting vascular endothelial growth factor.…”

Section: Tetrahedral Framework Nucleic Acids As Therapeutic Agentsmentioning

confidence: 99%

Therapeutic Applications of Programmable DNA Nanostructures

Aye

Sato

2022

Micromachines

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Deoxyribonucleic acid (DNA) nanotechnology, a frontier in biomedical engineering, is an emerging field that has enabled the engineering of molecular-scale DNA materials with applications in biomedicine such as bioimaging, biodetection, and drug delivery over the past decades. The programmability of DNA nanostructures allows the precise engineering of DNA nanocarriers with controllable shapes, sizes, surface chemistries, and functions to deliver therapeutic and functional payloads to target cells with higher efficiency and enhanced specificity. Programmability and control over design also allow the creation of dynamic devices, such as DNA nanorobots, that can react to external stimuli and execute programmed tasks. This review focuses on the current findings and progress in the field, mainly on the employment of DNA nanostructures such as DNA origami nanorobots, DNA nanotubes, DNA tetrahedra, DNA boxes, and DNA nanoflowers in the biomedical field for therapeutic purposes. We will also discuss the fate of DNA nanostructures in living cells, the major obstacles to overcome, that is, the stability of DNA nanostructures in biomedical applications, and the opportunities for DNA nanostructure-based drug delivery in the future.

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“…In recent years, an increasing number of researchers have applied tFNAs to tissue regeneration engineering to explore its multiple effects on cell biological behavior. It has been found that tFNAs has certain effects on cell proliferation, differentiation, migration, apoptosis, and other biological processes 28–30 . In this study, we attempt to use tFNAs as a carrier to deliver miRNA into cells, and synergistically promote osteogenic differentiation of ADSCs, so as to provide new research ideas and methods for bone tissue regeneration.…”

Section: Introductionmentioning

confidence: 99%

“…It has been found that tFNAs has certain effects on cell proliferation, differentiation, migration, apoptosis, and other biological processes. 28 , 29 , 30 In this study, we attempt to use tFNAs as a carrier to deliver miRNA into cells, and synergistically promote osteogenic differentiation of ADSCs, so as to provide new research ideas and methods for bone tissue regeneration.…”

Section: Introductionmentioning

confidence: 99%

MiR‐26a‐tetrahedral framework nucleic acids mediated osteogenesis of adipose‐derived mesenchymal stem cells

et al. 2022

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Objectives: Delivery systems that provide time and space control have a good application prospect in tissue regeneration applications, as they can effectively improve the process of wound healing and tissue repair. In our experiments, we constructed a novel micro-RNA delivery system by linking framework nucleic acid nanomaterials to micro-RNAs to promote osteogenic differentiation of mesenchymal stem cells. Materials and Methods:To verify the successful preparation of tFNAs-miR-26a, the size of tFNAs-miR-26a were observed by non-denaturing polyacrylamide gel electrophoresis and dynamic light scattering techniques. The expression of osteogenic differentiation-related genes and proteins was investigated by confocal microscope, PCR and western blot to detect the impact of tFNAs-miR-26a on ADSCs. And finally, Wnt/β-catenin signaling pathway related proteins and genes were detected by confocal microscope, PCR and western blot to study the relevant mechanism.Results: By adding this novel complex, the osteogenic differentiation ability of mesenchymal stem cells was significantly improved, and the expression of alkaline phosphatase (ALP) on the surface of the cell membrane and the formation of calcium nodules in mesenchymal stem cells were significantly increased on days 7 and 14 of induction of osteogenic differentiation, respectively. Gene and protein expression levels of ALP (an early marker associated with osteogenic differentiation), RUNX2 (a metaphase marker), and OPN (a late marker) were significantly increased. We also studied the relevant mechanism of action and found that the novel nucleic acid complex promoted osteogenic differentiation of mesenchymal stem cells by activating the canonical Wnt signaling pathway.Conclusions: This study may provide a new research direction for the application of novel nucleic acid nanomaterials in bone tissue regeneration.

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Tetrahedral framework nucleic acids-based delivery of microRNA-155 inhibits choroidal neovascularization by regulating the polarization of macrophages

Cited by 89 publications

References 64 publications

Prospects and challenges of dynamic DNA nanostructures in biomedical applications

Prospects and challenges of dynamic DNA nanostructures in biomedical applications

Therapeutic Applications of Programmable DNA Nanostructures

MiR‐26a‐tetrahedral framework nucleic acids mediated osteogenesis of adipose‐derived mesenchymal stem cells

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