Tetrahydroxy stilbene glucoside protected against diabetes‐induced osteoporosis in mice with streptozotocin‐induced hyperglycemia

Zhang, Jingjing; Chen, Xiangfan; Chen, Bingbing; Tong, Lijuan; Zhang, Yan

doi:10.1002/ptr.6240

Cited by 17 publications

(17 citation statements)

References 31 publications

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“…Reducing osteoclast differentiation-associated pathways by HSW may therefore represent a potential method for therapeutic intervention to prevent diabetes-related bone loss. In contrast, a previous study showed that THSG increases osteoblastogenic genes such as runt-related transcription factor 2 ( RUNX-2 ), OCN , and ALP in STZ-induced diabetic mice (Zhang et al, 2019). Our data did not show significant changes in RUNX-2 , OCN , and ALP between groups (data not shown).…”

Section: Resultsmentioning

confidence: 71%

“…Hwang et al (2016) and Kim et al (2018b) reported that Heshouwu hot water extract and tetrahydroxystilbene glucoside (THSG), which is a bioactive compound of Heshouwu, prevent bone loss in estrogen deficient mice. Zhang et al (2019) recently reported that THSG protects against diabetes-induced osteoporosis in mice with streptozotocin (STZ)-induced diabetes. However, it remains unknown if Heshouwu can regulate diabetes-induced bone loss in vivo , or the mechanism by which its effects occur.…”

Section: Introductionmentioning

confidence: 99%

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Heshouwu (Polygonum multiflorum Thunb.) Extract Attenuates Bone Loss in Diabetic Mice

Ham¹,

Lee²,

Choi³

et al. 2019

pnf

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This study investigated the effects and mechanism of Heshouwu (Polygonum multiflorum Thunb.) water extract (HSW) on diabetes-related bone loss in mice. HSW was orally administered (300 mg/kg body weight) to high-fat diet and streptozotocin-induced diabetic mice for 10 weeks. HSW significantly alleviated mouse body weight loss and hyperglycemia compared with the control group, and elevated serum levels of insulin, osteocalcin, and bone-alkaline phosphatase. HSW supplementation also significantly increased the bone volume/tissue volume ratio and trabecular thickness and number, and decreased the bone surface/bone volume ratio and trabecular structure model index in the femur and tibia. Moreover, HSW significantly increased femoral bone mineral density. In addition, HSW down-regulated osteoclastogenic genes, such as nuclear factor of activated T-cells, cytoplasmic 1 and tartrate-resistant acid phosphatase 5 (TRAP), in both the femur and tibia tissue, and reduced serum TRAP level compare to those of control mice. These results indicate that HSW might relieve diabetes-related bone disorders through regulating osteoclast-related genes, suggesting HSW may be used as a preventive agent for diabetes-induced bone loss.

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Section: Resultsmentioning

confidence: 71%

Section: Introductionmentioning

confidence: 99%

Heshouwu (Polygonum multiflorum Thunb.) Extract Attenuates Bone Loss in Diabetic Mice

Ham¹,

Lee²,

Choi³

et al. 2019

pnf

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“…The exact mechanism involved in molecular binding and biological cascade response between tanshinone IIA and renin protein need to be further identified. Previous studies have demonstrated that some natural products like salidroside and tetrahydroxy stilbene glucoside (Zhang et al 2019) could affect tissue ANG II level, while this study is the first to explore and report that the naturally occurring product tanshinone IIA from SMB could target renin activity, which, at least partially, explained that SMB could reduce ANG II-stimulated collagen synthesis in cardiac fibroblasts (Ling et al 2009).…”

Section: Discussionmentioning

confidence: 68%

“…Since high renin activity and enhanced ANG II expression contributed to the development of bone deteriorations associated with hyperglycaemia Zhang et al 2019), the beneficial effects of tanshinone IIA on bone tissue was evaluated in diabetic mice. Treatment of diabetic mice with tanshinone IIA markedly increased trabecular BMD and improved trabecular bone micro-architecture as well as enhanced trabecular bone area, in accordance with that tanshinone IIA exerted bone-sparing function in ovariectomized mice (Cheng et al 2018) and rats (Wang et al 2019).…”

Section: Discussionmentioning

confidence: 99%

Inhibition of tanshinone IIA on renin activity protected against osteoporosis in diabetic mice

Zhang

Cai

Yang

et al. 2020

Pharmaceutical Biology

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Context: Salvia miltiorrhiza Bge. (Labiatae) (SMB) is applied clinically for management of diabetic osteoporosis in China, and research results has suggested its potential action on renin-angiotensin system (RAS). Objective: This study screens and explores naturally occurring bioactive constituents from the root of SMB acting on renin activity and evaluates its osteoprotective efficacy in diabetic mice. Materials and methods: Human embryonic kidney (HEK) 293 cells, engineered to express human renin, were used as an in vitro model to identify bioactive compound, tanshinone IIA, inhibiting renin activity. The C57BL/6 mice (n ¼ 10 in each group) with diabetes induced by streptozotocin (STZ) were intraperitoneally injected with tanshinone IIA (10 and 30 mg/kg). The mice without STZ treatment and the diabetic mice treated with aliskiren were used as non-diabetic control and positive control, respectively. Results: Tanshinone IIA was found to display inhibitory effects on renin activity of HEK-293 cells; moreover, it down-regulated protein expression of ANG II in human renin-expressed HEK-293 cells. Treatment of diabetic mice with tanshinone IIA with both doses could significantly decrease ANG II level in serum (from 16.56 ± 1.70 to 10.86 ± 0.68 and 9.14 ± 1.31 pg/mL) and reduce ANG II expression in bone, consequently improving trabecular bone mineral density and micro-structure of proximal tibial end and increasing trabecular bone area of distal femoral end in diabetic mice. Conclusions: This study revealed beneficial effects of tanshinone IIA on bone of diabetic mice, and potentially suggested the application of Salvia miltiorrhiza in the treatment of osteoporosis and drug development of tanshinone IIA as a renin inhibitor.

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“…On the other hand, tetrahydroxy stilbene glucoside (TSG), which is an active component of P. multiflorum, was found to be effective in HFD-induced CF-1 diabetic mice [15]. Subsequent research indicated that TSG could inhibit of the tissue renin-angiotensin system (RAS), which plays a critical role in development of diabetic osteoporosis [16].…”

Section: Introductionmentioning

confidence: 99%

Water extract from processed Polygonum multiflorum modulate gut microbiota and glucose metabolism on insulin resistant rats

Yang

et al. 2020

BMC Complement Med Ther

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Background: The incidence of insulin resistance (IR) has rapidly increased worldwide over the last 20 years, no perfect solution has yet been identified. Finding new therapeutic drugs will help improve this situation. As a traditional Chinese medicine, PPM (processed Polygonum multiflorum) has widely been used in the clinic. Recently, other clinical functions of PPM have been widely analyzed. Results: Administration of the water extract from PPM decreased the level of FBG, TC, and TG, and increased the level of FGC, thereby reducing the IR index and improving IR. Furthermore, Western blot analysis revealed that PPM significantly increased GPR43 and AMPK expression when compared with the MOD group, and GPR43, AMPK were known as glucose metabolism-related proteins. In addition, treatment with PPM can restore the balance of gut microbiota by adjusting the relative abundance of bacteria both at the phylum and genus level, and these changes have been reported to be related to IR. Methods: Sprague Dawley (SD) rats were fed a high-fat diet and were gavaged daily with either normal saline solution or PPM for 12 weeks. Major biochemical indexes, such as fasting blood glucose (FBG), fasting glucagon (FGC), total cholesterol (TC), and triglyceride (TG) were measured. Then the protein expression of adenosine 5′monophosphate-activated protein kinase (AMPK) and G protein-coupled receptor 43 (GPR43) was evaluated by using Western blot analysis. Moreover, the composition of gut microbiota was assessed by analyzing 16S rRNA sequences. Conclusions: Our findings showed that PPM reversed the increasing of FBG and the decreasing of IRI, PPM accelerated the expression of glucose metabolism-related proteins and regulated the intestinal microecological balance. Therefore, we hold the opinion that PPM may be an effective option for treating IR.

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Tetrahydroxy stilbene glucoside protected against diabetes‐induced osteoporosis in mice with streptozotocin‐induced hyperglycemia

Cited by 17 publications

References 31 publications

Heshouwu (Polygonum multiflorum Thunb.) Extract Attenuates Bone Loss in Diabetic Mice

Heshouwu (Polygonum multiflorum Thunb.) Extract Attenuates Bone Loss in Diabetic Mice

Inhibition of tanshinone IIA on renin activity protected against osteoporosis in diabetic mice

Water extract from processed Polygonum multiflorum modulate gut microbiota and glucose metabolism on insulin resistant rats

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