Tetrapeptide KEDW Interacts with DNA and Regulates Gene Expression

Khavinson, Vladimir; Tendler, Solomon; Kasyanenko, N. A.; Tarnovskaya, S. I.; Линькова, Н С; Ashapkin, Vasily V.; Yakutseni, P. P.; Vanyushin, B. F.

doi:10.5099/aj150300156

Cited by 14 publications

(10 citation statements)

References 19 publications

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“…KEDW(Lys-Glu-Asp-Trp) peptide has the ability to induce differentiation and functional activity of various types of endocrine pancreatic cells. 20…”

Section: Introductionmentioning

confidence: 99%

“…19 KEDW(Lys-Glu-Asp-Trp) peptide has the ability to induce differentiation and functional activity of various types of endocrine pancreatic cells. 20 Thus, short peptides are involved in the regulation of proliferation and differentiation processes in various types of cells and tissues. The aim of this study was to investigate the effect of short peptides AED (Ala-Glu-Asp), KED, KE (Lys-Glu), and AEDG and also their mixtures on the initial stages of neuronal differentiation and proliferation of the primary culture of human periodontal ligament hPDLSCs.…”

Section: Introductionmentioning

confidence: 99%

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Effect of short peptides on neuronal differentiation of stem cells

Caputi

Trubiani

Sinjari

et al. 2019

Int J Immunopathol Pharmacol

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It has been demonstrated that short peptides play an important role in the transmission of biological information, modulation of transcription, and restoring genetically conditioned alterations occurring with age. Peptidergic regulation of homeostasis occupies an important place in physiological processes, which lead to the aging of cells, tissues, and organs, consisting in the involution of major regulatory systems-the nervous, the endocrine, and the immune. The effect of AED (Ala-Glu-Asp), KED (Lys-Glu-Asp), KE (Lys-Glu), AEDG (Ala-Glu-Asp-Gly) peptides and their compound on neuronal differentiation of human periodontal ligament stem cells (hPDLSCs) was studied by immunofluorescence and western blot analysis. Growth-Associated Protein 43 (GAP43), which implements neurotransmission mechanisms and neuroplasticity, demonstrated an increased expression in hPDLSCs cultured with a compound of all studied peptides and with KED alone. The peptide compound and KED, increase the expression of Nestin (neurofilament protein), expressed in early neuronal precursors in hPDLSCs cultures. Thus, the compound of peptides AEDG, KE, AED, and KED could promote the neuronal differentiation of hPDLSCs and be a promising tool for the study of peptides as a modulator of neurogenesis in neurodegenerative diseases studied in animal models.

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“…KEDW(Lys-Glu-Asp-Trp) peptide has the ability to induce differentiation and functional activity of various types of endocrine pancreatic cells. 20…”

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Effect of short peptides on neuronal differentiation of stem cells

Caputi

Trubiani

Sinjari

et al. 2019

Int J Immunopathol Pharmacol

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“…Results obtained through physical methods (UV-visible absorption, circular dichroism) and molecular modelling methods suggest that the peptide binds to DNA along the major groove. Experimental and theoretical data provided a 3D model of a stable DNA-peptide complex [43]. Another peptide, AEDL, regulates the levels of Ki67, Mcl-1, p53, CD79, NOS-3 proteins in cell cultures of human bronchial epithelium in various passages.…”

Section: Discussionmentioning

confidence: 99%

Neuroprotective Effect of EDR Peptide in Mouse Model of Huntington's Disease

Хавинсон¹,

Линькова²,

Kukanova³

et al. 2017

J Neurol Neurosci

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Huntington's disease (HD) is a fatal, inherited neurodegenerative disorder. The study in functioning and aging of neurons may give an opportunity to regulate these processes. Previous investigations demonstrated the ability of EDR peptide (Glu-Asp-Arg) to penetrate a cell nucleus and stimulate gene expression. The data obtained prompt EDR peptide capability to restore the morphology of spines in striatum neurons in Huntington's disease (HD) mouse model. EDR peptide has been shown by us to bind the DNA in solution (absorption spectroscopy and dynamic light scattering) and in a computer model. The proposed model suggests that EDR peptide binds specific binding site oligo (dCG) along the DNA minor groove.

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“…The study of the properties of the peptide KEDW by means of UV spectroscopy, circular dichroism, and molecular modeling has demonstrated that this tetrapeptide binds to the DNA in association with the sequence ACCT, which is found in the promoter regions of genes responsible for the functional state of pancreatic cells. The authors used this result to present an explanation of the previously identified properties of the tetrapeptide [42].…”

Section: Short Peptides + Dnamentioning

confidence: 98%

“…It has been demonstrated that the AEDL peptide induces the differentiation of lung cells by regulating the expression of the NKX2-1, SCGB1A1, SCGB3A2, FOXA1, and FOXA2 genes [41]. Another study showed that the peptide KEDW increases the expression of the genes PDX1, NGN3, MNX1, PAX6, FOXA2, NKX2-2, NKX6.1, HOXA3, and PAX4 that are important for maintaining the functional activity of pancreatic endocrine cells [42]. It has been established that directed differentiation of immune cells is stimulated by the peptides KE, DS, (Nα-(γ-E) -E), K (H-E-OH) -OH, AED, KED, EDA, and KEDG [34].…”

Section: Peptide Regulation Of Cell Differentiationmentioning

confidence: 99%

Peptide Regulation of Gene Expression: A Systematic Review

et al. 2021

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Peptides are characterized by their wide range of biological activity: they regulate functions of the endocrine, nervous, and immune systems. The mechanism of such action of peptides involves their ability to regulate gene expression and protein synthesis in plants, microorganisms, insects, birds, rodents, primates, and humans. Short peptides, consisting of 2–7 amino acid residues, can penetrate into the nuclei and nucleoli of cells and interact with the nucleosome, the histone proteins, and both single- and double-stranded DNA. DNA–peptide interactions, including sequence recognition in gene promoters, are important for template-directed synthetic reactions, replication, transcription, and reparation. Peptides can regulate the status of DNA methylation, which is an epigenetic mechanism for the activation or repression of genes in both the normal condition, as well as in cases of pathology and senescence. In this context, one can assume that short peptides were evolutionarily among the first signaling molecules that regulated the reactions of template-directed syntheses. This situation enhances the prospects of developing effective and safe immunoregulatory, neuroprotective, antimicrobial, antiviral, and other drugs based on short peptides.

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Tetrapeptide KEDW Interacts with DNA and Regulates Gene Expression

Cited by 14 publications

References 19 publications

Effect of short peptides on neuronal differentiation of stem cells

Effect of short peptides on neuronal differentiation of stem cells

Neuroprotective Effect of EDR Peptide in Mouse Model of Huntington's Disease

Peptide Regulation of Gene Expression: A Systematic Review

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