Tetrazolium test to evaluate the viability of oil radish seeds

Abstract

ABSTRACT:The use of tetrazolium test is important in the evaluation of seeds lot quality and it has been adopted for vigor and viability identification for several species. The interest on the production of oil radish is increasing since the seeds were considered a good source of oil for biofuel production. The development of the tetrazolium test methodology for seeds of this species can improve the seed quality control process, and additionally will provide information for the characterization of remaining seeds (dead or dormant) in the germination tests. To verify the ideal conditions to tetrazolium test was conducted two experiments. At the first, oil radish seeds cultivar CATI AL-1000, lots from 2001 and 2006 were submitted to imbibition between paper in water for 6 hours. After the longitudinal cut in the longest direction, the seeds were immersed in the tetrazolium solution at the concentrations of 0,075%, 0,5% and 1% at 25°C for 3h, 12h and 18h. In the second experiment, oil radish seeds cultivar CATI AL-1000 lot from 2005 and IPR 116 cultivar, lots from 2004 and 2005 were immersed in the concentrations of ,1%; 0,2%; 0,3% and 0,4% of tetrazolium solution for 12 hours at a 25°C, 30°C, 35°C and 40°C. At the first experiment was observed the necessity of test intermediate concentrations between 0,075% and 0,5%, since with 0,075% the seeds stained weakly and with 0,5% the test results, were overestimated. In the second experiment was observed that the 0,3% concentration at 30°C can be recommended for the utilization of tetrazolium test to evaluation of oil radish seeds viability.

Tetrazolium test to assess the viability of kale seeds

“…Similar results indicating the need to remove the seed coat were also obtained by Barros et al (2005) in zucchini. However, in seeds of forage turnip (Nery et al, 2015), canola (Flores et al, 2015) and carrot (Lima et al, 2018), the longitudinal cut along the longest axis before immersion in the tetrazolium solution was the most indicated.…”

“…Therefore, this concentration was eliminated from the subsequent tests. Several authors (Rezende et al, 2015;Flores et al, 2015;Nery et al, 2015) reported difficulty in interpreting the test when the seeds were exposed to a concentration of 1.0%, because they showed a very intense red staining. This fact brought challenges to the interpretation of the test, especially when associated with prolonged times of exposure.…”

“…The efficiency of the tetrazolium test in the evaluation of seed viability depends on the development of appropriate methods for each species, determining adequate hydration conditions, preparation of seeds, concentration of the tetrazolium solution, as well as priming time and temperature (Fantazzini et al, 2020). Some authors working with several species of vegetables, such as crambe (Rezende et al, 2015), forage turnip (Nery et al, 2015), canola (Flores et al, 2015) and coriander (Silva et al, 2021), have found that it is necessary to define the criteria for interpreting the staining and for classifying seeds for viability and vigor.…”

Methodological adjustments to the tetrazolium test in coriander seeds

“…Several authors (Lima et al, 2010;Flores et al, 2015;Nery et al, 2015 andVera et al, 2019) reported difficulty interpreting the test when the seeds were exposed to a concentration of 1.0%, as they presented a very intense red color. This fact posed challenges to the interpretation of the test, especially when associated with prolonged times of exposure and combination with temperatures above 40 °C.…”