TFAP2B, AP-1 and JAZF1 Expression in Tissues of Papillary Thyroid Carcinoma Patients; Clinical, Pathological and Prognostic Values

Abstract

Objective: Transcription factor activating protein 2 B (TFAP2 B) is a transcription factor that regulates many steps of embryogenesis, cell growth, apoptosis and recently oncogenesis and cancer progression. AP-1 is a transcription factor that is a downstream molecule of the MAPK signaling pathway. Juxtaposed with zinc finger gene 1 (JAZF1) is a recently detected transforming growth factor which has a role in carcinogenesis. Hence the present study aimed to assess those markers expression in tissues from patients with such cancer correlation their expression with clinic-pathological findings of the tumor and prognostic and follow-up findings of patients. Methods: We have collected tissue samples from papillary thyroid cancer patients and adjacent non-neoplastic tissues from 80 patients. We assessed the expression of TFAP2B, AP-1 and JAZF1 using immunohistochemistry. Results: Expression of TFAP2B was positively associated with lymph nodes metastases (p=0.003), distant metastases (p=0.002), recurrence of the tumor (p=0.002), unfavourable disease-free survival rate (p=0.003). AP-1 expression is positively associated with advanced stage (p=0.002), presence of extra-thyroid invasion (p=0.005), recurrence of the tumor (p=0.005), unfavorable disease-free survival rate (p=0.01). JAZF1 expression is negatively associated with huge tumor size (0.023), vascular invasion (p=0.007) and unfavorable overall survival rate (p=.030). Conclusion: High expression levels of TFAP2B and AP-1 and low expression levels of JAZF1 were associated with unfavourable pathological, prognostic parameters and dismal patient's outcome.

Up-regulation of NGEF by ERK/AP1 signaling pathway in BRAFV600E-mutant thyroid cancer promotes cancer metastasis

“…Notably AP-1 is a transcription factor complex composed of c-Fos and c-Jun dimers, and is a key regulator of tumor cell invasion [47]. AP-1 promotes migration and invasion in a variety of different tumor cell types, such as triple-negative breast, colorectal and thyroid cancer [48][49][50][51][52][53]. Although AP-1 is involved in multiple pathways associated with cancer [52][53][54], the detailed molecular mechanisms are yet to be fully elucidated.…”

“…AP-1 promotes migration and invasion in a variety of different tumor cell types, such as triple-negative breast, colorectal and thyroid cancer [48][49][50][51][52][53]. Although AP-1 is involved in multiple pathways associated with cancer [52][53][54], the detailed molecular mechanisms are yet to be fully elucidated. Collectively, results of the present study demonstrated that NGEF is a downstream gene of AP-1 associated with BRAF V600E -mutant thyroid cancer metastasis.…”

To Explore the Inhibitory Mechanism of Quercetin in Thyroid Papillary Carcinoma through Network Pharmacology and Experiments

“…5 Disease Markers JUN, a key component of dimeric transcription factor activator protein-1 (AP-1), plays a role in the transcriptional response to extracellular signaling [37]. A previous study showed that AP-1 expression in papillary thyroid carcinoma cancer tissue is positively correlated with a high incidence of disease progression, a high tumor recurrence rate, and unfavorable disease-free survival [38]. In our experimental group, JUN expression was downregulated in PTC cells after Disease Markers quercetin treatment.…”