TGF-  and p53 Staining in CT-Guided and Endoscopic Ultrasound Fine-Needle Aspirates of Pancreatic Adenocarcinoma

Abstract

Our purpose was to determine if fine-needle aspirates of pancreatic adenocarcinoma would produce material amenable to tumor marker staining and to correlate the expression of TGF-beta and p53 with patient and tumor data. One hundred twenty specimens were analyzed. TGF-beta was positive in 26% of cases and had no correlation with patient's age, sex, survival, stage, grade, or size. p53 was positive in 22% of the cases and correlated only with grade 1 tumors. Expression of TGF-beta and p53 can be tested on preserved cytologic specimens. This is the largest study to date correlating TGF-beta and p53 expression in pancreatic adenocarcinoma and patient demographics, prognosis, and tumor attributes. This is also the first study that did not select for surgical candidates. TGF-beta expression does not appear to have prognostic significance in pancreatic cancer. p53 was more common in well-differentiated tumors and may be an early mutation lost in more poorly differentiated tumors.

Expression of transforming growth factor-beta-1 and p27Kip1 in pancreatic adenocarcinomas: relation with cell-cycle-associated proteins and clinicopathologic characteristics

“…In most series, the presence of TGF-β1 is associated with shorter postoperative survival [34], although in some other studies TGF-β1 expression showed a significant correlation with longer survival time [19,29]. On the other hand, there are reports indicating that TGF-β expression has no association with survival [39]. In the present study, to have the same treatment protocol only the patients which had RO resections were considered in survival analysis and TGF-β1 and p27 expression did not show a significant influence on the survival of patients.…”

Role of endoscopic ultrasound in the diagnosis and staging of pancreatic cancer

“…Only one study demonstrated the feasibility of immunocytochemistry for the assessment of p53 and transforming growth factor-b expression in the cytology samples stored as slides at the time of initial diagnosis by EUS-or CT-guided FNA. 182 The genetic profile of EUS-FNA samples has been analyzed through macro-array analysis that included 1,176 genes associated with cancer, by using commercially available complementary DNA samples. 183 A complex statistical analysis was performed (hierarchical clustering, principal component analysis, significance analysis of microarrays) that allowed initial establishment of the gene expression.…”

Clinical Utility and Diagnostic Accuracy of Endoscopic Ultrasound-Guided Fine Needle Aspiration of Pancreatic Lesions: Saudi Arabian Experience

“…Recently we have started to focus more on the importance of cell-block preparation and now we dedicate 1 full pass for that purpose. Additional samples are sent in as needed for microbiology cultures and other ancillary studies [7]. Cell-block material was fixed in 10% buffered neutral formalin and the sections (4–5 µm) from paraffin-embedded cell block were cut and stained with hematoxylin and eosin.…”