TGFβ-1 Induced Cross-Linking of the Extracellular Matrix of Primary Human Dermal Fibroblasts

Semkova, Mariya E.; Hsuan, J. Justin

doi:10.3390/ijms22030984

Cited by 17 publications

(12 citation statements)

References 74 publications

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“…Transforming growth factor-β (TGF-β) is a key regulator of ECM biosynthesis [ 53 ]. Especially, the TGFβ/Smad pathway is mainly responsible for the collagen synthesis in human dermal fibroblasts.…”

Section: Resultsmentioning

confidence: 99%

“…Especially, the TGFβ/Smad pathway is mainly responsible for the collagen synthesis in human dermal fibroblasts. TGF-β controls collagen homeostasis by regulating the Smad pathway [ 53 ]. First, TGF-β binds to a TGF-β type II receptor (TβRII), which can be associated with a TGF-β type I receptor (TβRI) and lead to its phosphorylation.…”

Section: Resultsmentioning

confidence: 99%

“…Activated Smad2 or Smad3 bind to Smad4 for forming heteromeric Smad complexes. These Smad complexes move to the nucleus and interact with Smad-binding elements to induce the transcription process of procollagen genes [ 53 , 54 ]. UV irradiation can decrease procollagen synthesis by suppressing the TGF-β/Smad signaling pathway [ 55 , 56 ].…”

Section: Resultsmentioning

confidence: 99%

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The Molecular Mechanism of Polyphenols with Anti-Aging Activity in Aged Human Dermal Fibroblasts

2022

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Skin is the largest organ in the body comprised of three different layers including the epidermis, dermis, and hypodermis. The dermis is mainly composed of dermal fibroblasts and extracellular matrix (ECM), such as collagen and elastin, which are strongly related to skin elasticity and firmness. Skin is continuously exposed to different kinds of environmental stimuli. For example, ultraviolet (UV) radiation, air pollutants, or smoking aggravates skin aging. These external stimuli accelerate the aging process by reactive oxygen species (ROS)-mediated signaling pathways and even cause aging-related diseases. Skin aging is characterized by elasticity loss, wrinkle formation, a reduced dermal-epidermal junction, and delayed wound healing. Thus, many studies have shown that natural polyphenol compounds can delay the aging process by regulating age-related signaling pathways in aged dermal fibroblasts. This review first highlights the relationship between aging and its related molecular mechanisms. Then, we discuss the function and underlying mechanism of various polyphenols for improving skin aging. This study may provide essential insights for developing functional cosmetics and future clinical applications.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

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The Molecular Mechanism of Polyphenols with Anti-Aging Activity in Aged Human Dermal Fibroblasts

2022

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“…CAFs generate contractile forces on the ECM, which is known to result in the release and activation of TGF-β1 [ 179 ], and activation of TGF-β signalling in fibroblasts, which in turn upregulates various cross-linking enzymes in the ECM, including TG2 that has a further impact on the biomechanical environment by increasing tissue stiffness [ 96 ]. Mechanical stresses can also directly induce and stabilize an open catalytically active conformation of TG2; hence, active TG2 results in a cross-linked ECM that can act as a storage depot for TGF-β [ 180 ].…”

Section: Tg2 Is Key To the Biomechanical Progression Of Cancermentioning

confidence: 99%

The Biological and Biomechanical Role of Transglutaminase-2 in the Tumour Microenvironment

Tempest

Guarnerio

Maani

et al. 2021

Cancers

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Transglutaminase-2 (TG2) is the most highly and ubiquitously expressed member of the transglutaminase enzyme family and is primarily involved in protein cross-linking. TG2 has been implicated in the development and progression of numerous cancers, with a direct role in multiple cellular processes and pathways linked to apoptosis, chemoresistance, epithelial-mesenchymal transition, and stem cell phenotype. The tumour microenvironment (TME) is critical in the formation, progression, and eventual metastasis of cancer, and increasing evidence points to a role for TG2 in matrix remodelling, modulation of biomechanical properties, cell adhesion, motility, and invasion. There is growing interest in targeting the TME therapeutically in response to advances in the understanding of its critical role in disease progression, and a number of approaches targeting biophysical properties and biomechanical signalling are beginning to show clinical promise. In this review we aim to highlight the wide array of processes in which TG2 influences the TME, focussing on its potential role in the dynamic tissue remodelling and biomechanical events increasingly linked to invasive and aggressive behaviour. Drug development efforts have yielded a range of TG2 inhibitors, and ongoing clinical trials may inform strategies for targeting the biomolecular and biomechanical function of TG2 in the TME.

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“…It is reported that TGF-β induced the upregulation of Wnt/β-catenin signalling in hypertrophic scar and keloid fibroblasts. The role of TGFβ in impairing ECM remodeling and antiangiogenic resistance ( Baek et al, 2001 ) has been demonstrated in various fibrotic models ( Semkova and Hsuan, 2021 ).…”

Section: Discussionmentioning

confidence: 99%

Enhanced healing of oral chemical burn by inhibiting inflammatory factors with an oral administration of shengFu oil

Yin¹,

Hong²,

Tang³

et al. 2022

Front. Pharmacol.

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ShengFu oil is a compounded Chinese medicinal prescription, and provides antibacterial, anti-inflammatory, and analgesic effects, favoring burn wound repair. In this study, we aimed at investigating the effects of topical applications of ShengFu oil and its active ingredients in oral chemical burns and elucidating its regulatory effects on β-catenin, COX-2, and MMP-9 expression caused by exposure to acid or alkaline agents. ShengFu oil contains 16 components, such as Frankincense, Radix Scutellariae and Radix Rehmanniae, and the main active ingredients from Frankincense are α-pinene, linalool, and n-octanol. Mouse models of oral chemical burns were induced by using glacial acetic acid or sodium hydroxide. Hematoxylin and eosin staining and immunohistochemical staining were used to detect the protein expressions of β-catenin, COX-2, and MMP-9 in wound tissues. They were further quantified by multispectral imaging analysis to clarify the effective mechanism of ShengFu oil for intervening inflammatory factors and active components. Our results indicated that the application of ShengFu oil on oral chemical burns effectively stopped the oral burn bleeding and reduced the inflammatory reaction in the damaged tissues, demonstrating that ShengFu oil can promote wound tissue repair in burns caused by heat, acids, and alkalis. The immunohistochemical staining results illustrated that ShengFu oil and its active ingredients significantly reversed the abnormal changes in inflammation-related proteins in mouse tongue tissues that were caused by chemical burns. Regarding long-term toxic effects of ShengFu oil on the gastrointestinal tract, liver, and kidney system, the results of hematoxylin and eosin staining experiments depicted that ShengFu oil was safe and effective for liver, kidney, intestine, esophagus, and tongue. All of these demonstrated that ShengFu oil and its active ingredients are effective and safe in preventing and treating oral chemical burns by interfering with the inflammatory microenvironment.

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TGFβ-1 Induced Cross-Linking of the Extracellular Matrix of Primary Human Dermal Fibroblasts

Cited by 17 publications

References 74 publications

The Molecular Mechanism of Polyphenols with Anti-Aging Activity in Aged Human Dermal Fibroblasts

The Molecular Mechanism of Polyphenols with Anti-Aging Activity in Aged Human Dermal Fibroblasts

The Biological and Biomechanical Role of Transglutaminase-2 in the Tumour Microenvironment

Enhanced healing of oral chemical burn by inhibiting inflammatory factors with an oral administration of shengFu oil

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