Th‐17 response and antimicrobial peptide expression are uniformly expressed in gastric mucosa of <i>Helicobacter pylori</i>‐infected patients independently of their clinical outcomes

Cremniter, Julie; Bodet, Charles; Tougeron, David; Dray, Xavier; Guilhot, Joëlle; Jégou, Jean‐François; Morel, Franck; Lecron, Jean‐Claude; Silvain, Christine; Burucoa, Christophe

doi:10.1111/hel.12479

Cited by 4 publications

(6 citation statements)

References 43 publications

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“…Adamsson et al reported that the level of expression of the genes encoding the prototype Th17 cytokine IL‐17A and the Th1 lymphokine IFN‐γ is enhanced in the corpus and antrum of Swedish patients with H. pylori infection compared to uninfected individuals; more importantly they confirmed by immunofluorescence that the number of IL‐17A + and IFN‐γ + cells is also increased in H. pylori ‐infected patients . Similar to that, a significant increase in IL17A mRNA transcripts was observed in the gastric tissue of H. pylori ‐infected patients from Iran and France compared to uninfected ones. Arachchi et al also reported that H. pylori infection is associated with elevated IL‐17 serum concentrations in a human cohort from Sri‐Lanka .…”

Section: Immunologymentioning

confidence: 71%

“…However, two studies showed that the number of infiltrating Th17 cells is higher in H. pylori ‐infected patients with peptic ulcers compared to individuals with H. pylori gastritis . To converse, Cremniter et al described that the same level of IL17A mRNA is found in patients with gastritis, ulcer, or precancerous and cancerous lesions …”

Section: Immunologymentioning

confidence: 99%

“…29,30 To converse, Cremniter et al described that the same level of IL17A mRNA is found in patients with gastritis, ulcer, or precancerous and cancerous lesions. 31 An alternative picture to the Th1/Th17 synergy and antagonism 33,34 They found that MAIT cells were enhanced in the gastric mucosa of patients or mice infected with H. pylori. 35 Transgenic Vα19iCα −/− Mr1 +/+ mice, which express only the TCRα chain (Vα19i) harbored by MAIT cells, 36 exhibited high levels of MAIT cells in the stomach and showed increased gastritis compared to Vα19iCα −/− Mr1 −/− mice upon H. pylori infection.…”

Section: The Proinflammatory Response Characterized By the Th1 Andmentioning

confidence: 99%

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Helicobacter: Inflammation, immunology, and vaccines

et al. 2018

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Helicobacter pylori infection induces a chronic gastric inflammation which can lead to gastric ulcers and cancer. The mucosal immune response to H. pylori is first initiated by the activation of gastric epithelial cells that respond to numerous bacterial factors, such as the cytotoxin-associated gene A or the lipopolysaccharide intermediate heptose-1,7-bisphosphate. The response of these cells is orchestrated by different receptors including the intracellular nucleotide-binding oligomerization domain-containing protein 1 or the extracellular epidermal growth factor receptor. This nonspecific response leads to recruitment and activation of various myeloid (macrophages and dendritic cells) and T cells (T helper-17 and mucosal-associated invariant T cells), which magnify and maintain inflammation. In this review, we summarize the major advances made in the past year regarding the induction, the regulation, and the role of the innate and adaptive immune responses to H. pylori infection. We also recapitulate efforts that have been made to develop efficient vaccine strategies.

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Section: Immunologymentioning

confidence: 71%

Section: Immunologymentioning

confidence: 99%

Section: The Proinflammatory Response Characterized By the Th1 Andmentioning

confidence: 99%

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Helicobacter: Inflammation, immunology, and vaccines

et al. 2018

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“…To extrapolate quantitative results for biopsies, a standard curve for PCR was implemented using ten-fold geometric serial dilutions (1 × 10 −1 to 1 × 10 −7 ) of a CFU-numbered suspension of a 48 h-cultured H. pylori J99-type strain [ 28 , 29 ]. Presence of H. pylori was determined by detecting the glmM gene specific for this bacterial species.…”

Section: Methodsmentioning

confidence: 99%

“…The amount of eCFU was numbered using the calibration curve. Absence of PCR inhibitors was assessed by detection of a 110-bp-long fragment of a human housekeeping gene (β-globin) [ 28 ].…”

Section: Methodsmentioning

confidence: 99%

Where to Biopsy to Detect Helicobacter pylori and How Many Biopsies Are Needed to Detect Antibiotic Resistance in a Human Stomach

Pichon

Tran

Motillon

et al. 2020

JCM

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This study aims to determine the gastric distribution, density, and diversity of Helicobacter pylori infection. Subtotal resection of the stomachs of three H. pylori-infected and asymptomatic obese patients were collected after a sleeve gastrectomy. Distribution and density of H. pylori were determined using culture and RT-PCR on multiple gastric sites (88, 176, and 101 biopsies per patient). Diversity of H. pylori strains was studied using antibiotic susceptibility testing, random amplified polymorphism DNA (RAPD) typing and cagA gene detection on single-colony isolates (44, 96, and 49 isolates per patient). H. pylori was detected in nearly all analyzed sites (354/365 biopsies, 97%). Antral density was higher in one patient only. The three stomachs were almost exclusively infected by an antibiotic-susceptible strain. One clarithromycin-resistant isolate in one biopsy was detected in two stomachs (1/44 and 1/49 isolates), while in the third one, eight (8/96 isolates) metronidazole-resistant isolates were detected. DNA typing showed infection with cagA-negative strains for one patient, cagA-positive strains for a second patient and the third patient was infected with two different strains of distinct cagA genotypes. Infection with H. pylori is shown to spread to the whole surface of the stomach, but a possibility of minor sub-population of antibiotic-resistant clones, undetectable in routine practice.

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The global prevalence of gastric cancer in Helicobacter pylori-infected individuals: a systematic review and meta-analysis

Shirani,

Pakzad,

Haddadi

et al. 2023

BMC Infect Dis

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Background Helicobacter pylori is a gastrointestinal pathogen that infects around half of the world's population. H. pylori infection is the most severe known risk factor for gastric cancer (GC), which is the second highest cause of cancer-related deaths globally. We conducted a systematic review and meta-analysis to assess the global prevalence of GC in H. pylori-infected individuals. Methods We performed a systematic search of the PubMed, Web of Science, and Embase databases for studies of the prevalence of GC in H. pylori-infected individuals published from 1 January 2011 to 20 April 2021. Metaprop package were used to calculate the pooled prevalence with 95% confidence interval. Random-effects model was applied to estimate the pooled prevalence. We also quantified it with the I2 index. Based on the Higgins classification approach, I2 values above 0.7 were determined as high heterogeneity. Results Among 17,438 reports screened, we assessed 1053 full-text articles for eligibility; 149 were included in the final analysis, comprising data from 32 countries. The highest and lowest prevalence was observed in America (pooled prevalence: 18.06%; 95% CI: 16.48 − 19.63; I2: 98.84%) and Africa (pooled prevalence: 9.52%; 95% CI: 5.92 − 13.12; I2: 88.39%). Among individual countries, Japan had the highest pooled prevalence of GC in H. pylori positive patients (Prevalence: 90.90%:95% CI: 83.61–95.14), whereas Sweden had the lowest prevalence (Prevalence: 0.07%; 95% CI: 0.06–0.09). The highest and lowest prevalence was observed in prospective case series (pooled prevalence: 23.13%; 95% CI: 20.41 − 25.85; I2: 97.70%) and retrospective cohort (pooled prevalence: 1.17%; 95% CI: 0.55 − 1.78; I 2: 0.10%). Conclusions H. pylori infection in GC patients varied between regions in this systematic review and meta-analysis. We observed that large amounts of GCs in developed countries are associated with H. pylori. Using these data, regional initiatives can be taken to prevent and eradicate H. pylori worldwide, thus reducing its complications.

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Th‐17 response and antimicrobial peptide expression are uniformly expressed in gastric mucosa of Helicobacter pylori‐infected patients independently of their clinical outcomes

Cited by 4 publications

References 43 publications

Helicobacter: Inflammation, immunology, and vaccines

Helicobacter: Inflammation, immunology, and vaccines

Where to Biopsy to Detect Helicobacter pylori and How Many Biopsies Are Needed to Detect Antibiotic Resistance in a Human Stomach

The global prevalence of gastric cancer in Helicobacter pylori-infected individuals: a systematic review and meta-analysis

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