Th1-type epitopes-based cocktail PDDV attenuates hepatic fibrosis in C57BL/6 mice with chronic Schistosoma japonicum infection

Tao, Fangfang; Yang, Yanfen; Wang, Hui; Sun, Xin; Luo, Jie; Zhu, Xin‐Guang; Li, Feng; Wang, Yong; Su, Chuan; Wu, Hongxun; Zhang, Zhao-Song

doi:10.1016/j.vaccine.2009.04.073

Cited by 18 publications

(17 citation statements)

References 26 publications

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“…The BABL/c mice were infected cutaneously with 14±2 S. japonicum cercariae [21].The liver fibrosis models were established after the mice being infected for 8 weeks and 15 weeks, respectively. Intragastric administrations of PZQ (250 mg/kg/24 hours) for 3 days and continuing 1% carboxymethyl cellulose carrier up to 30 days were carried out as an anti-parasite intervention [16].…”

Section: Methodsmentioning

confidence: 99%

“…Images of six random microscopic fields of red- stained collagen fibers in the liver section of each mouse were recorded using an inverted microscope (ZEISS, Goettingen, Germany) and then digitized and analyzed on Image-Pro Plus software 6.0 as previously described [21]. The content of liver hydroxyproline was determined according to the protocol of the Hydroxyproline Testing Kit (Jiancheng, Nanjing, China) as described [23].…”

Section: Methodsmentioning

confidence: 99%

“…Images of six random microscopic fields in the sections of each mouse were recorded using an inverted microscope (ZEISS, Goettingen, Germany) and the α-SMA positive area of each image was analyzed by Image-Pro Plus software 6.0 software [21].…”

Section: Methodsmentioning

confidence: 99%

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New Insight into the Antifibrotic Effects of Praziquantel on Mice in Infection with Schistosoma japonicum

et al. 2011

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BackgroundSchistosomiasis is a parasitic disease infecting more than 200 million people in the world. Although chemotherapy targeting on killing schistosomes is one of the main strategies in the disease control, there are few effective ways of dealing with liver fibrosis caused by the parasite infection in the chronic and advanced stages of schistosomiasis. For this reason, new strategies and prospective drugs, which exert antifibrotic effects, are urgently required.Methods and FindingsThe antifibrotic effects of praziquantel were assessed in the murine models of schistosomiasis japonica. Murine fibrosis models were established by cutaneous infection with 14±2 Schistosoma japonicum cercariae. Then, the mice of both chronic (8 weeks post-infection) and advanced (15 weeks post-infection) schistosomiasis were treated by gavage of praziquantel (250 mg/kg, once daily for 3 days) to eliminate worms, and followed by praziquantel anti-fibrosis treatment (300 mg/kg, twice daily for 30 days). The fibrosis-related parameters assessed were areas of collagen deposition, content of hydroxyproline and mRNA expressions of Col1α1, Col3α1, α-SMA, TGF-β, MMP9, TIMP1, IL-4, IL-10, IL-13 and IFN-γ of liver. Spleen weight index, alanine aminotransferase activity and liver portal venous pressure were also measured. The results showed that anti-fibrosis treatment improved liver fibrosis, splenomegaly, hepatic function, as well as liver portal hypertension. In order to confirm the anti-fibrotic properties of praziquantel, we established a CCL4-induced model and revealed that CCL4-induced liver fibrosis was inhibited by PZQ treatment for 30 days. Furthermore, we analyzed the effects of praziquantel on mouse primary hepatic stellate cells (HSCs). It is indicated that mRNA expressions of Col1α1, Col3α1, α-SMA, TGF-β, MMP9 and TIMP1 of HSCs were all inhibited after praziquantel anti-parasite treatments.ConclusionsThe significant amelioration of hepatic fibrosis by praziquantel treatment validates it as a promising drug of anti-fibrosis and offers potential of a new chemotherapy for hepatic fibrosis resulting from schistosomiasis.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

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New Insight into the Antifibrotic Effects of Praziquantel on Mice in Infection with Schistosoma japonicum

et al. 2011

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“…Some of the lead S. japonicum vaccine candidates (as recombinant protein and/or DNA vaccines) are presented in Table 1. Many are membrane proteins, muscle components, or enzymes, and further details of the characteristics and efficacies, where tested, of these and other vaccine candidates can be found elsewhere (96,137,138,139,180,212). Recent studies in water buffaloes of the protection afforded by S. japonicum recombinant proteins (e.g., paramyosin Despite this wealth of information on vaccine discovery and efficacy, the current S. japonicum vaccine candidates may prove not to be the most effective, and it is therefore important to continue to identify new target antigens.…”

Section: Vaccine Developmentmentioning

confidence: 99%

Schistosomiasis in the People's Republic of China: the Era of the Three Gorges Dam

et al. 2010

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SUMMARY The potential impact of the Three Gorges Dam (TGD) on schistosomiasis transmission in China has invoked considerable global concern. The TGD will result in changes in the water level and silt deposition downstream, favoring the reproduction of Oncomelania snails. Combined with blockages of the Yangtze River's tributaries, these changes will increase the schistosomiasis transmission season within the marshlands along the middle and lower reaches of the Yangtze River. The changing schistosome transmission dynamics necessitate a comprehensive strategy to control schistosomiasis. This review discusses aspects of the epidemiology and transmission of Schistosoma japonicum in China and considers the pathology, clinical outcomes, diagnosis, treatment, immunobiology, and genetics of schistosomiasis japonica together with an overview of current progress in vaccine development, all of which will have an impact on future control efforts. The use of synchronous praziquantel (PZQ) chemotherapy for humans and domestic animals is only temporarily effective, as schistosome reinfection occurs rapidly. Drug delivery requires a substantial infrastructure to regularly cover all parts of an area of endemicity. This makes chemotherapy expensive and, as compliance is often low, a less than satisfactory control option. There is increasing disquiet about the possibility that PZQ-resistant schistosomes will develop. Consequently, as mathematical modeling predicts, vaccine strategies represent an essential component in the future control of schistosomiasis in China. With the inclusion of focal mollusciciding, improvements in sanitation, and health education into the control scenario, China's target of reducing the level of schistosome infection to less than 1% by 2015 may be achievable.

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“…infections (Kofta and Wedrychowicz, 2001). The strategies of vaccine for schistosomiasis are to find new candidate antigens or enhance the existing antigens by using cocktail vaccine or different adjuvants as well as better injection technique (Tao et al, 2009;Tian et al, 2011;Wei et al, 2008Wei et al, , 2009. Most vaccine studies have focused attention on how to prevent infection of people by pathogens, but ignored the fact that those people living in endemic areas were already infected by Schistosoma more or less.…”

Section: Discussionmentioning

confidence: 99%

Schistosoma japonicum scFv-IL18 fusion DNA ameliorates hepatic fibrosis in schistosomiasis-infected mice via improving local concentration of IL-18 in liver

XiYa

Zhou

Qimei

et al. 2013

Experimental Parasitology

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Th1-type epitopes-based cocktail PDDV attenuates hepatic fibrosis in C57BL/6 mice with chronic Schistosoma japonicum infection

Cited by 18 publications

References 26 publications

New Insight into the Antifibrotic Effects of Praziquantel on Mice in Infection with Schistosoma japonicum

New Insight into the Antifibrotic Effects of Praziquantel on Mice in Infection with Schistosoma japonicum

Schistosomiasis in the People's Republic of China: the Era of the Three Gorges Dam

Schistosoma japonicum scFv-IL18 fusion DNA ameliorates hepatic fibrosis in schistosomiasis-infected mice via improving local concentration of IL-18 in liver

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