Th17/Treg cell expression in children with primary nephritic syndrome and the effects of ox-LDL on Th17/Treg cells

Abstract

ABSTRACT.To investigate the role of T-helper cells/Treg (Th17/ Treg) and morbidity factors related to primary nephritic syndrome (PNS) in children, as well as the influence of ox-low density lipoprotein (ox-LDL) on Th17/Treg expression in children with PNS. To clarify the pathogenesis of PNS in children, 50 children with PNS treated in our hospital were enrolled in the study group. Additionally, 20 healthy children who came to our hospital for physical examination during the same period were enrolled in the control group. Th17 and Treg cells in children belonging to the two groups were detected by flow cytometry; the numbers of Th17/Treg cells in peripheral blood mononuclear cells at different concentrations of ox-LDL were detected simultaneously. Ox-LDL can affect the number of Th17/Treg cells in peripheral blood mononuclear cells, and both cell types decreased with increasing concentration of ox-LDL, with the numbers being significantly lower in the control group. However, the decrease in the number of Th17 cells was statistically insignificant (P > 0.05), whereas the decrease in Treg cells was more obvious and statistically significant (P < 0.05). The effect of ox-LDL the number of Treg cells was stronger than that on Th17 cells. We concluded that the imbalance of Th17/Treg cells influenced by high and low ox-LDL concentrations in children with PNS might be the immunological basis of the disease.

Efficacy and safety of mycophenolate mofetil versus levamisole in frequently relapsing nephrotic syndrome: an open-label randomized controlled trial

“…The pathogenesis of minimal change disease and the basis for immunosuppressive therapies are unclear. Because studies suggest an imbalance of Th1/Th2 cytokines, 30,31 Th17/Treg dysregulation, [32][33][34][35][36] and abnormalities in B cells, 37,38 we examined the proportions of B and T lymphocyte subsets by flow cytometry in patients receiving MMF or levamisole. Similar to previous reports, we found that disease relapse was associated with Th2 39 and Th17 [33][34][35] up-regulation, and down-regulation of Treg cells.…”

“…Because studies suggest an imbalance of Th1/Th2 cytokines, 30,31 Th17/Treg dysregulation, [32][33][34][35][36] and abnormalities in B cells, 37,38 we examined the proportions of B and T lymphocyte subsets by flow cytometry in patients receiving MMF or levamisole. Similar to previous reports, we found that disease relapse was associated with Th2 39 and Th17 [33][34][35] up-regulation, and down-regulation of Treg cells. 34,36 Contrary to reports of Th1 up-regulation with levamisole, 40 and B cell 41 and Th17 42 down-regulation and Treg up-regulation 43 with MMF, we did not find differences in lymphocyte subsets at follow-up evaluation.…”

AGE-DEPENDENT CHANGES OF T-REGULATORY AND Th17 SUBSET LEVELS IN PERIPHERAL BLOOD FROM HEALTHY HUMANS

“…Нам не удалось найти исследования, прослеживаю-щего изменения уровня Th17 от рождения и до очень пожилого возраста, однако наши данные хорошо корреспондируют с уровнем Th17 в кон-трольных группах в многочисленных исследова-ниях субпопуляции Th17 при различных заболе-ваниях у людей разного возраста [13,19,20,27]. У здоровых детей грудного и младшего возраста Th17 определяются на уровне 1-3% [13,19], а у пожилых людей их количество прогрессивно на-растает с возрастом [27]. Однако сопоставление данных литературы осложняется тем, что в раз-ных работах применяли разные способы детекции Th17-лимфоцитов.…”

“…Многие авторы для анализа баланса иммун-ной системы предлагают использовать отноше-ние Treg/Th17 [13,19,20,27]. Мы провели соот-ветствующие расчеты.…”

“…4), достоверность аппрокси-мации R 2 = 0,98, что свидетельствует об очень вы-сокой степени соответствия модели. Большин-ство авторов отмечает увеличение уровня Th17 при изучаемой ими патологии, или, по крайней мере, уменьшение отношения Treg/Th17 [2,13,19,20,27]. Мы также исследовали детей с забо-леваниями различной этиологии, в основе кото-рых лежит воспаление, и всякий раз обнаружива-ли повышение уровня Th17 (неопубликованные данные).…”