The 16S and COI Mitochondrial DNA Nucleotide Composition of Stripped Snakehead (Channa striata Bloch,1793) from Lake Sentani, Jayapura, Papua

Abstract

Stripped snakehead (Channa striata Bloch,1793) has potency to be developed in Indonesia due to high nutritional value including omega 3 which is useful for children growth. However, study on composition of mitochondrial DNA nucleotide as a part of study on genetic characterization of stripped snakehead fish in Indonesia is poorlyunderstood. Genetic characterization of stripped snakehead is important to obtain data information that can be applied for breeding and conservation program. Therefore, the objective of this study was to determine the 16S and COI mitochondrial DNA nucleotide of stripped snakehead collected from Lake Sentani, Jayapura, Papua. Method used in this research is PCR method using primer 16Sar and 16Sbr for 16S mitochondrial gene, and FishF2 and FishR2 for COI mitochondrial gene. The results showed that composition of 16S mtDNA nucleotide from 616 bp was T=21.92%, C=25.49%, A=30.19%, dan G=22.40%, whereas composition of A+T=52.11% and G+C=47.89%. In addition, composition of COI mtDNA nucleotide from 705 bp was T=29.93%, C=28.65%, A=24.26%, and G=17.16% while composition of A+T=54.48% and G+C=45.52%. Data of 16S and COI mitochondrial DNA nucleotide composition of the stripped snakehead collected from Lake Sentani gained in this study are to be expected to complete genetic information of the fish species which is useful to improve breeding program of this fish species in the future.

Genetic Identification of Two Mudskipper Species (Oxudercidae: <i>Periophthalmus</i>) from Kulon Progo, Special Region of Yogyakarta, Indonesia

“…We found that P. kalolo samples from Bogowonto were in the same clade as samples from Baros Beach (MZ614852, MZ614847), Cilacap (KU692753), but in a different clade compared to samples from Baros Beach (MZ614840-42, MZ614844-46, MZ614848-49, MZ614850-51, MZ614853-54), Tulungagung (KU692750, KU692751, KU692751, KU692755, KU692747, KU692748), and other samples from Cilacap (KU692754). These results are consistent with previous research by Arisuryanti et al (2018) and Aji and Arisuryanti (2021). Additionally, we also observed that P. argentilineatus from Baros Beach (MZ606684-86) were in the same clade as samples from Pandeglang (KU692744-46) and samples from Bogowonto (MT4359598-600), separated from the clade with P. argentilineatus from Baros Beach (MZ606687) and a sample from Tukad Bilukpoh, Jembrana Bali (KU692743) (Dahruddin et al 2017;Aji & Arisuryanti 2021).…”

“…This result is consistent with the study by Ward et al (2005) which discovered a total GC content COI ranging from 42.2-47.1%. Additionally, we found a T>C>A>G nucleotide composition pattern of the COI gene that was comparable to studies in other fish families (Bingpeng et al 2018;Kombong & Arisuryanti 2018;Wu et al 2018;Linh et al 2018;Aji & Arisuryanti 2021).…”

“…The taxonomic classification of members within the family Oxudercidae (previously known as Gobiidae) is often hindered by their morphological similarities, presenting significant difficulties in species identification (Thacker 2003). This phenomenon has been observed in mudskippers of the genus Boleophthalmus and Periophthalmus, with numerous reports of cryptic species (Chen et al 2014;Polgar et al 2014;Arisuryanti et al 2018;Aji & Arisuryanti 2021;Rha'ifa et al 2021). Using traditional morphological methods alone has proven to be insufficient in accurately identifying Periophthalmus species, as evidenced by several misidentifications such as Periophthalmus walailakae with Periophthalmodon schlosseri (1), Periophthalmus takita, Jaafar & Larson, 2008with Periophthalmus novaeguineaensis Eggert, 1935, and Periophthalmus variabilis Eggert, 1935 with Periophthalmus novemradiatus Hamilton, 1822 (3) (Murdy 1989;Jaafar et al 2006;Jafaar & Larson 2008;Jaafar et al 2009).…”

“…Pasir Mendit Beach in Kulon Progo, Special Region of Yogyakarta, Indonesia is a unique and valuable ecosystem, encompassing both a tourism and conservation area with dense mangroves and muddy terrain. It is located near the coastline of Yogyakarta, close to Muara Bogowonto and Baros Beach, the habitats for several species of mudskipper (Arisuryanti et al 2018;Aji & Arisuryanti 2021). Despite its significance, the genetic information of the mudskipper populations in Pasir Mendit Beach is yet to be explored.…”

Identification, genetic diversity, and comparative evolution of the striped snakehead Channa striata (Bloch, 1793) in Wallacea, Indonesia

“…This study showed that the Channa striata COI nucleotide composition pattern is T>C>A>G, a pattern typical within the Teleostei and the Pisces superclass in general (Chen et al 2016;Osho et al 2021), but contrast with base composition of the C. striata from Thailand (Boonkusol and Tongbai 2016), and different from the nucleotide composition of some fishes such as the Asian seabass (Lates calcarifer) (Irmawati et al 2020). The same COI nucleotide pattern was also reported by Kombong and Arisuryanti (2018) in C. striata in Lake Sentani Jayapura Papua and in African snakehead Parachanna obscura in Nigeria's freshwater (Osho et al 2021). The guanine and cytosine (GC) content of C. striata in this study was less than 50%, ranging from 45.60% to 46.21%.…”