The 2.2 kb E1b mRNA of human Ad12 and Ad5 codes for two tumor antigens starting at different AUG triplets

Role of adenovirus types 5 and 12 early region 1 b tumor antigens in oncogenic transformation

“…Gmstruction of an Ad12 plasmid defective in expression of the f9kD Elb polypeptide. To construct an Ad12 El plasmid with a mutation at the AccI site at position 1594 of the Ad12 sequence (mapping between the first and second ATG of Ad12 Elb), 20 pg of pAdl2RIC (Bos et al, 1981) was partially digested with 3 units of AccI for 30 min at 37". The B-protruding AccI ends were repaired with Klenow enzyme as described above.…”

“…Recently, it was shown that the two Elb proteins are translated from different reading frames which are only partially overlapping (Bos et al, 1981). This arrangement makes it possible to mutate one of the two Elb proteins without affecting the other, so that the contribution in oncogenicity of each of the Elb products can be measured separately.…”

Acylation of adenovirus type 12 early region Ib 18‐kDa protein

“…The region of the adenovirus 12 (Ad 12) chromosome whose expression is sufficient to induce transformation, early region 1, consists of two transcriptional units: Ela and Elb [7,8]. The former codes for a family of proteins of molecular mass of about 41 kDa, as determined by SDSpolyacrylamide gel electrophoresis (SDS-PAGE), whereas the latter codes for two proteins of molecular masses 19 and 60 kDa [9, lo] (equivalent to proteins of 18 and 52 kDa, respectively, in our hands).…”

Deletion mutants of region E1 a of AD12 E1 plasmids: Effect on oncogenic transformation

“…Cell labeling with [?S]methionine and subsequent immunoprecipitation was per-formed as described previously (Schrier et , 1979). The constru'ction of pAdl2 RIC (or pRIC) has been described by Bos et al, (1981). The Ad12 Ela mutants pR7, pR8, and pRl1 are derived from this plasmid by deleting the RruI site (AGTACT at position 1003) using standard recombinant DNA techniques.…”