The abnormal eosinophils are part of the leukemic cell population in acute myelomonocytic leukemia with abnormal eosinophils (AML M4Eo) and carry the pericentric inversion 16: a combination of May-Grunwald- Giemsa staining and fluorescence in situ hybridization

Abstract

The French-American-British subtype acute myelomonocytic leukemia with abnormal eosinophils (FAB AML M4Eo) with pericentric inversion of chromosome 16 is cytomorphologically defined by a myelomonoblastic blast population and abnormal eosinophils. Until now, it remained an open question whether these abnormal eosinophils are part of the malignant clone or an epiphenomenon. We analyzed five cases of AML M4Eo with inv(16) and combined May-Grunwald-Giemsa staining with fluorescence in situ hybridization using yeast artificial chromosome clone 854E2, which spans the inv(16) breakpoint on 16p. In the case of inv(16), three instead of the normal two hybridization signals can be observed both on metaphase spreads and in interphase cells. With this approach, we were able to show inversion 16 in abnormal eosinophils and, therefore, identified them as a part of the leukemic cell population.

Which compartments are involved in Philadelphia‐chromosome positive chronic myeloid leukaemia? An answer at the single cell level by combining May‐Grünwald‐Giemsa staining and fluorescence in situ hybridization techniques

“…A mixture of biotinylated ABL DNA and digoxigenin-labelled mBCR DNA was used as provided by the manufacturers (mBCR/ABL probe, Oncor, Gaithersburg, U.S.A.). FISH was performed according to Haferlach et al (1996). The BCR gene at chromosome 22q11 was identified by a green fluorescent spot, and the ABL gene at chromosome 9q34 by a red spot (Zeiss Axioskop ® , Jena, Germany).…”

New insights into the biology of Philadelphia‐chromosome‐positive acute lymphoblastic leukaemia using a combination of May‐Grünwald‐Giemsa staining and fluorescence in situ hybridization techniques at the single cell level

“…Fluorescence in situ hybridization. FISH was performed using a commercially available bcr/acl translocation probe kit (mBCR/ABL translocation probe ® , Vysis, Stuttgart, Germany) and performed according to Haferlach et al (1996Haferlach et al ( , 1997. The probe for the bcr gene region at chromosome 22q11 was labelled with SpectrumGreen ® fluorophore and the abl probe was labelled with SpectrumOrange ® fluorophore.…”

Evaluation of flow cytometric assessment of myeloid nuclear differentiation antigen expression as a diagnostic marker for myelodysplastic syndromes in a series of 269 patients

“…CM was performed on May-Gruenwald-Giemsa stained BM slides. Additionally, cytochemistry was performed using iron staining, staining with myeloperoxidase and non-specific esterase using alphanaphtyl-acetate (30)(31)(32). Diagnoses were made according to FAB criteria (33)(34)(35) and especially following the WHO classification system (6,36).…”