The Absence of the “GATC - Binding Protein SeqA” Affects DNA Replication in Salmonella enterica Serovar Typhimurium

Aloui, Amine; Saloua, Kouass Sahbani; Mihoub, Mouadh; Alya, El May; Landoulsi, Ahmed

doi:10.5772/21249

Cited by 7 publications

(6 citation statements)

References 46 publications

(34 reference statements)

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“…Thus, H. pylori turned out to be tremendously more susceptible to VDP1 than the other bacterial species. This is likely to be explained by the difference in the predominant fatty acid molecules attached to the PE of the respective bacteria: myristic acid is the predominant molecule attached to H. pylori PE, while palmitic acid is the predominant molecule attached to the PE of all of the other bacteria except for S. aureus that does not possess PE 15 16 17 18 19 20 21 .…”

Section: Resultsmentioning

confidence: 99%

Identification and characterization of a vitamin D3 decomposition product bactericidal against Helicobacter pylori

Hosoda

Shimomura

Wanibuchi

et al. 2015

Sci Rep

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This study demonstrated that the vitamin D3 decomposition product VDP1 exerts an antibacterial action against Helicobacter pylori but not against other bacteria. Treatment with VDP1 induced a collapse of cell membrane structures of H. pylori and ultimately lysed the bacterial cells. A unique dimyristoyl phosphatidylethanolamine in the membrane lipid compositions contributed to the interaction of VDP1 with H. pylori cells. In separate experiments, VDP1 had no influence on the viability of the human cancer cell lines MKN45 and T47D and lacked any vitamin D3-like hormonal action against the latter. In both 1H and 13C NMR analyses, the spectra patterns of VDP1 corresponded with those of Grundmann's ketone. These results suggest that VDP1 (or Grundmann's ketone-type indene compound) may become a fundamental structure for the development of new antibacterial substances with selective bactericidal action against H. pylori.

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Section: Resultsmentioning

confidence: 99%

Identification and characterization of a vitamin D3 decomposition product bactericidal against Helicobacter pylori

Hosoda

Shimomura

Wanibuchi

et al. 2015

Sci Rep

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“…A number of studies by other groups have demonstrated that the most prevalent saturated fatty acid component of PE found in typical Gram-negative bacteria, such as E. coli, Klebsiella pneumoniae, Salmonella enterica serovar Typhimurium, and Pseudomonas aeruginosa, is a palmitic acid (C 16:0 ) (1,5,13,24). The most prevalent saturated fatty acid component of the PE in E. coli analyzed in this study was also C 16:0 .…”

Section: Discussionmentioning

confidence: 99%

Phosphatidylethanolamine of Helicobacter pylori Functions as a Steroid-Binding Lipid in the Assimilation of Free Cholesterol and 3 -Hydroxl Steroids into the Bacterial Cell Membrane

Shimomura

Hosoda

Hayashi

et al. 2012

Journal of Bacteriology

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“…A number of investigations, including our own, have revealed that the most predominant saturated fatty‐acid side chain of PtdEtn of typical Gram‐negative bacteria is a palmitic acid (16:0) (Amine et al, ; Dunnick & O'Leary, ; Kearns et al, ; Shimomura et al, ; Shokri & Larsson, ). Meanwhile, an earlier investigation by other group has demonstrated that a Gram‐positive bacterium S. aureus does not possess PtdEtn (Beining, Huff, Prescott, & Theodore, ).…”

Section: Discussionmentioning

confidence: 99%

“…A previous study by our group has identified dimyristoyl‐phosphatidylethanolamine (di‐14:0 PtdEtn) as one of the most predominant glycerophospholipid constituents of the cell membranes of H. pylori (Shimomura, Hosoda, Hayashi, Yokota, & Hirai, ). A number of studies, including our own, have demonstrated a complete absence of di‐14:0 PtdEtn in the bacterial membranes of representative Gram‐negative bacteria such as Enterobacteriaceae bacteria and Pseudomonas aeruginosa (Amine, Saloua, Mouadh, Alya, & Ahmed, ; Dunnick & O'Leary, ; Kearns, Robinson, & Shimkets, ; Shimomura et al, ; Shokri & Larsson, ). di‐14:0 PtdEtn was thus shown to be a characteristic glycerophospholipid of H. pylori alone.…”

Section: Introductionmentioning

confidence: 90%

Indene Compounds Synthetically Derived from Vitamin D Have Selective Antibacterial Action on Helicobacter pylori

Wanibuchi

Hosoda²,

Ihara

et al. 2018

Lipids

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Helicobacter pylori infects the human stomach and is closely linked with the development of gastric cancer. When detected, this pathogen can be eradicated from the human stomach using wide-spectrum antibiotics. However, year by year, H. pylori strains resistant to the antibacterial action of antibiotics have been increasing. The development of new antibacterial substances effective against drug-resistant H. pylori is urgently required. Our group has recently identified extremely selective bactericidal effects against H. pylori in (1R,3aR,7aR)-1-[(1R)-1,5-dimethylhexyl]octahydro-7a-methyl-4H-inden-4-one (VDP1) (otherwise known as Grundmann's ketone), an indene compound derived from the decomposition of vitamin D and proposed the antibacterial mechanism whereby VDP1 induces the bacteriolysis by interacting at least with PtdEtn (dimyristoyl-phosphatidylethanolamine [di-14:0 PtdEtn]) retaining two 14:0 fatty acids of the membrane lipid constituents. In this study, we synthesized new indene compounds ((1R,3aR,7aR)-1-((2R,E)-5,6-dimethylhept-3-en-2-yl)-7a-methyloctahydro-4H-inden-4-one [VD2-1], (1R,3aR,7aR)-1-((S)-1-hydroxypropan-2-yl)-7a-methyloctahydro-1H-inden-4-ol [VD2-2], and (1R,3aR,7aR)-7a-methyl-1-((R)-6-methylheptan-2-yl)octahydro-1H-inden-4-ol [VD3-1]) using either vitamin D or vitamin D as materials. VD2-1 and VD3-1 selectively disrupted the di-14:0 PtdEtn vesicles without destructing the vesicles of PtdEtn (dipalmitoyl-phosphatidylethanolamine) retaining two 16:0 fatty acids. In contrast, VD2-2, an indene compound lacking an alkyl group, had no influence on the structural stability of both PtdEtn vesicles. In addition, VD2-1 and VD3-1 exerted extremely selective bactericidal action against H. pylori without affecting the viability of commonplace bacteria. Meanwhile, VD2-2 almost forfeited the bactericidal effects on H. pylori. These results suggest that the alkyl group of the indene compounds has a crucial conformation to interact with di-14:0 PtdEtn of H. pylori membrane lipid constituents whereby the bacteriolysis is ultimately induced.

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The Absence of the “GATC - Binding Protein SeqA” Affects DNA Replication in Salmonella enterica Serovar Typhimurium

Cited by 7 publications

References 46 publications

Identification and characterization of a vitamin D3 decomposition product bactericidal against Helicobacter pylori

Identification and characterization of a vitamin D3 decomposition product bactericidal against Helicobacter pylori

Phosphatidylethanolamine of Helicobacter pylori Functions as a Steroid-Binding Lipid in the Assimilation of Free Cholesterol and 3 -Hydroxl Steroids into the Bacterial Cell Membrane

Indene Compounds Synthetically Derived from Vitamin D Have Selective Antibacterial Action on Helicobacter pylori

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