The acid adaptive tolerance response in <scp>C</scp>ampylobacter jejuni induces a global response, as suggested by proteomics and microarrays

Varsaki, Athanasia; Murphy, Caroline; Barczynska, Alicja; Jordan, Kieran; Carroll, C.

doi:10.1111/1751-7915.12302

Cited by 19 publications

(14 citation statements)

References 67 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Organic acids are regularly used to remove foodborne bacteria from poultry carcasses in the United States [28,29,30]. However, many foodborne pathogens have acid stress mechanisms that can help them adapt to varying pH environments [38,65,66,67,68]. Campylobacter jejuni has the gene make-up required to induce an acid-tolerance response [68].…”

Section: Discussionmentioning

confidence: 99%

“…However, many foodborne pathogens have acid stress mechanisms that can help them adapt to varying pH environments [38,65,66,67,68]. Campylobacter jejuni has the gene make-up required to induce an acid-tolerance response [68]. In addition, there is an increase of acid tolerance in C. jejuni caused by coincubation with protozoa [69], and the genes involved in iron control and uptake are also induced [67,68].…”

Section: Discussionmentioning

confidence: 99%

“…Campylobacter jejuni has the gene make-up required to induce an acid-tolerance response [68]. In addition, there is an increase of acid tolerance in C. jejuni caused by coincubation with protozoa [69], and the genes involved in iron control and uptake are also induced [67,68]. We evaluated the interactions of six different OAs—propionic, l -lactic, formic, citric, butyric, and acetic—against 96 C. jejuni potential foodborne pathogen strains, while evaluating the effects that pH and the undissociated and dissociated OA species have on the bacteria at their OA MIC M s.…”

Section: Discussionmentioning

confidence: 99%

See 2 more Smart Citations

Inhibition and Interactions of Campylobacter jejuni from Broiler Chicken Houses with Organic Acids

et al. 2019

View full text Add to dashboard Cite

Campylobacter jejuni is a bacterium that causes major diarrheal disease worldwide and is also one of the top five foodborne pathogens encountered in the United States. Poultry is a major source of C. jejuni, and a high-risk factor for contracting campylobacteriosis. Organic acids are used in the United States during food animal processing for removal of bacterial contamination from animal carcasses. Six organic acids were evaluated in inhibition studies of 96 C. jejuni strains obtained from shoe covers used in broiler chicken houses at different poultry farms in several states by determining the susceptibilities of the C. jejuni strains, along with the pH values at the molar minimum inhibitory concentrations (MICMs). The undissociated and dissociated organic acid concentrations were calculated at the MICMs with the Henderson-Hasselbalch equation. The results for the 96 C. jejuni strains were treated similarly for each different organic acid. Campylobacter jejuni inhibition did correlate with the dissociated organic acids, but did not correlate with pH or with the undissociated organic acids. When the concentrations of dissociated organic acids decreased, the C. jejuni strains were not disinfected. A carcass wash using organic acids should have the concentration of dissociated acid species carefully controlled. It is suggested to maintain a dissociated acid concentration for propionic, l-lactic, formic, citric, butyric, and acetic acids at 24, 40, 36, 21, 23, and 25 mM, respectively, and at these dissociated organic acid levels an acid wash would be expected to remove or inhibit 97% or more of the C. jejuni bacteria studied here. However, studies must be undertaken to confirm that the suggested concentrations of dissociated organic acids are adequate to remove C. jejuni bacteria in the field vs. the laboratory. Due to propionate, l-lactate, formate, butyrate, and acetate being utilized by C. jejuni, these organic acids may not be appropriate for use as a carcass wash to remove C. jejuni surface contamination. Of all tested organic acids, dissociated citric acid was the most efficient at inhibiting C. jejuni.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Inhibition and Interactions of Campylobacter jejuni from Broiler Chicken Houses with Organic Acids

et al. 2019

View full text Add to dashboard Cite

show abstract

“…Iron uptake in C. jejuni may be generally related to acid tolerance and antibiotic survival. Transcriptomic and proteomic studies have shown that this iron uptake system, in addition to several other iron uptake genes ( cfbpA / B , ceuE , and chuZ ), was more highly expressed upon C. jejuni exposure to acid stress ( 74 , 75 ). In addition, deletion of fur increased C. jejuni sensitivity to acidic conditions ( 76 ).…”

Section: Discussionmentioning

confidence: 99%

Investigating the Campylobacter jejuni Transcriptional Response to Host Intestinal Extracts Reveals the Involvement of a Widely Conserved Iron Uptake System

Liu

Boinett

Chan

et al. 2018

mBio

View full text Add to dashboard Cite

Campylobacter jejuni is a pathogenic bacterium that causes gastroenteritis in humans yet is a widespread commensal in wild and domestic animals, particularly poultry. Using RNA sequencing, we assessed C. jejuni transcriptional responses to medium supplemented with human fecal versus chicken cecal extracts and in extract-supplemented medium versus medium alone. C. jejuni exposed to extracts had altered expression of 40 genes related to iron uptake, metabolism, chemotaxis, energy production, and osmotic stress response. In human fecal versus chicken cecal extracts, C. jejuni displayed higher expression of genes involved in respiration (fdhTU) and in known or putative iron uptake systems (cfbpA, ceuB, chuC, and CJJ81176_1649–1655 [here designated 1649–1655]). The 1649–1655 genes and downstream overlapping gene 1656 were investigated further. Uncharacterized homologues of this system were identified in 33 diverse bacterial species representing 6 different phyla, 21 of which are associated with human disease. The 1649 and 1650 (p19) genes encode an iron transporter and a periplasmic iron binding protein, respectively; however, the role of the downstream 1651–1656 genes was unknown. A Δ1651–1656 deletion strain had an iron-sensitive phenotype, consistent with a previously characterized Δp19 mutant, and showed reduced growth in acidic medium, increased sensitivity to streptomycin, and higher resistance to H2O2 stress. In iron-restricted medium, the 1651–1656 and p19 genes were required for optimal growth when using human fecal extracts as an iron source. Collectively, this implicates a function for the 1649–1656 gene cluster in C. jejuni iron scavenging and stress survival in the human intestinal environment.

show abstract

“…It has been suggested that C. jejuni possesses a robust regulatory system and stress-protective mechanisms, which enable its survival in hostile conditions within and outside the host [Svensson et al, 2008]. For subsets of genes that encode proteins, a global change in gene expression was observed in responses to heat shock, alkaline shock, and oxidative stress during the stationary phase [Wright et al, 2009]; however, C. jejuni lacks the RNA polymerase sigma factor (RpoS), which regulates stress response in the stationary phase in other bacteria [Turonova et al, 2017;Varsaki et al, 2015]. In addition to the above-mentioned stress factors, C. jejuni must overcome aerobic conditions.…”

Section: Qs and Stress Responsesmentioning

confidence: 99%

Molecules Autoinducer 2 and cjA and Their Impact on Gene Expression in Campylobacter jejuni

et al. 2018

View full text Add to dashboard Cite

Quorum sensing is a widespread form of cell-to-cell communication, which is based on the production of signaling molecules known as autoinducers (AIs). The first group contains highly species-specific N-acyl homoserine lactones (N-AHLs), generally known as AI-1, which are produced by AHL synthase. The second group, possessing the characteristic structure of a furanone ring, are known as AI-2. The enzyme responsible for their production is S-ribosylhomocysteine lyase (LuxS). In Campylobacter jejuni, AI-2 and LuxS play a role in many important processes, including biofilm formation, stress response, motility, expression of virulence factors, and colonization. However, neither the receptor protein nor the exact structure of the AI-2 molecule have been identified to date. Similarly, little is known about the possible existence of AHL-synthase producing AI-1 and its impact on gene expression. Recently, an analogue of homoserine lactone, called cjA, was isolated from a cell-free supernatant of C. jejuni strain 81–176 and from the food isolate c11. The molecule cjA particularly impacted the expression of virulence factors and biofilm formation. This review summarizes the role of AI-2 and cjA in the context of biofilm formation, motility, stress responses, and expression of virulence factors.

show abstract

The acid adaptive tolerance response in Campylobacter jejuni induces a global response, as suggested by proteomics and microarrays

Cited by 19 publications

References 67 publications

Inhibition and Interactions of Campylobacter jejuni from Broiler Chicken Houses with Organic Acids

Inhibition and Interactions of Campylobacter jejuni from Broiler Chicken Houses with Organic Acids

Investigating the Campylobacter jejuni Transcriptional Response to Host Intestinal Extracts Reveals the Involvement of a Widely Conserved Iron Uptake System

Molecules Autoinducer 2 and cjA and Their Impact on Gene Expression in <b><i>Campylobacter jejuni</i></b>

Contact Info

Product

Resources

About