The acid test for pH-dependent gating in cloned HV1 channels

Islas, León D

doi:10.1085/jgp.201812076

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Cited by 2 publications

(2 citation statements)

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“…Different molecular mechanism of ΔpH sensing are currently under debate 47 . Protonation or deprotonation of amino-acid residues can induce conformational changes in proteins.…”

Section: Discussionmentioning

confidence: 99%

Voltage and pH difference across the membrane control the S4 voltage-sensor motion of the Hv1 proton channel

Schladt

Berger

2020

Sci Rep

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The voltage-gated proton channel Hv1 is expressed in a variety of cells, including macrophages, sperm, and lung epithelial cells. Hv1 is gated by both the membrane potential and the difference between the intra- and extracellular pH (ΔpH). The coupling of voltage- and ∆pH-sensing is such that Hv1 opens only when the electrochemical proton gradient is outwardly directed. However, the molecular mechanism of this coupling is not known. Here, we investigate the coupling between voltage- and ΔpH-sensing of Ciona intestinalis proton channel (ciHv1) using patch-clamp fluorometry (PCF) and proton uncaging. We show that changes in ΔpH can induce conformational changes of the S4 voltage sensor. Our results are consistent with the idea that S4 can detect both voltage and ΔpH.

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“…Different molecular mechanism of ΔpH sensing are currently under debate 47 . Protonation or deprotonation of amino-acid residues can induce conformational changes in proteins.…”

Section: Discussionmentioning

confidence: 99%

Voltage and pH difference across the membrane control the S4 voltage-sensor motion of the Hv1 proton channel

Schladt

Berger

2020

Sci Rep

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“…Our results support in several lines the model described in ref. 91 for the observed ΔpH dependence of hH V 1 gating. This model suggests that protonation sites at the entrance of the intra- and extracellular cavities regulate the position of the S4 helix.…”

Section: Resultsmentioning

confidence: 82%

The pH-dependent gating of the human voltage-gated proton channel from computational simulations

Jardin

Ohlwein

Franzen

et al. 2022

Phys. Chem. Chem. Phys.

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The acid test for pH-dependent gating in cloned HV1 channels

Abstract: Islas evaluates two papers that provide mechanistic insight into pH-dependent gating in HV1 proton channels.

Cited by 2 publications

References 14 publications

Voltage and pH difference across the membrane control the S4 voltage-sensor motion of the Hv1 proton channel

Voltage and pH difference across the membrane control the S4 voltage-sensor motion of the Hv1 proton channel

The pH-dependent gating of the human voltage-gated proton channel from computational simulations

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