The Acousto-Optical Method for Blood Typing (Part 1). Photometric and Statistical Methods for Image Processing

Doubrovski, V. A.; Medvedeva, M. F.

doi:10.1007/s10527-016-9577-3

Cited by 7 publications

(6 citation statements)

References 8 publications

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“…To an extent this sample is an equivalent of the case of erythrocytes agglutination negative reaction. Introduction of such test is determined by desire to improve reliability of blood typing, that is one of the principal differences of this work from [15,20] and others. Indeed, for blood typing of the examined blood sample with three experiments carrying out with US exposure to the samples, only one of them is a priori known as the one without agglutination reaction.…”

Section: Materials and Experimental Techniquementioning

confidence: 99%

“…In the works [20,21] the blood typing is performed using hemagglutinating serums based on the following techniques of photo images processing: photometric approach; static technique, including analysis of dispersion of pixels distribution for certain observation zone by their brightness, count of number of photo image pixels with the set brightness, calculation of summary brightness of photo image pixels. Comparison of the obtained resolution values for AOM of blood typing was performed for the above mentioned techniques of photo image processing.…”

Section: Introductionmentioning

confidence: 99%

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Digital Processing Techniques Influence on Resolution of Acousto-Optical Blood Typing Method

Doubrovski

Markov

2022

Optics and Spectroscopy

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The various digital techniques of photo images processing influence upon the resolution of the acousto-optical method (AOM) for instrumental blood typing according to the AB0 system has been studied. Experiments with blood samples were carried out using monoclonal antibodies (tsoliclones) of anti-A and anti-B types, as well as without them ("clean" sample) when the mixture was exposing simultaneously by a standing ultrasonic wave. The experiment was recorded by a digital camera. Different variants of statistical calculations have been proposed to process the photo images of the recorded phenomena, the results were compared with the traditional photometric approach. It is shown that the statistical processing of experimental results gives a higher resolution in blood group determination and, consequently, a higher reliability of its typing. A comparison of different variants for statistical photo frames processing was carried out to study the possibility to increase the resolution of AOM in blood typing. Keywords: acousto-optical blood typing method, monoclonal antibodies, digital processing techniques, blood typing, high resolution.

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Section: Materials and Experimental Techniquementioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Digital Processing Techniques Influence on Resolution of Acousto-Optical Blood Typing Method

Doubrovski

Markov

2022

Optics and Spectroscopy

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“…This method depends on the distribution of green scale on image pixels ( ) and computes the number of brighten one to determine the blood group type [17]. Digital color images taken and the region of interest area selected.…”

Section: Cuvette Sample Analysesmentioning

confidence: 99%

“…First step are taking the G component only from the RGB image, then select brightness threshold pixel value ( ℎ ) for the selected area, which is considered the comparting value between negative and positive agglutination. On the other hand, number of bright image pixels ( ) that have the brightness value over than the threshold value calculated as [17]:…”

Section: Cuvette Sample Analysesmentioning

confidence: 99%

Enhancement the Agglutination of Erythrocytes in Blood Typing Test by Acousto-Optic Technique

Ali

Hasan

Khalil

2020

NJES

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A proposed modern technique for determination the blood group typing by monitoring the agglutination of red blood cells using acousto-optical technique and digital camera. The method based on analysis the digital image of the agglutination process by MATLAB software. We present an overview of two acousto-optic sensing approaches; the first demonstrates the cuvette approach while the second is the microscope slide approach. The cuvette approach digital image analyzing depends on the green channel distribution of the original image and count the brighten pixels, while the microscope slide approach passes through series of algorithms started with grayscale filter and end with edge detection it counts the different color pixels. The experimental result shown that it is possible to enhance the determination of blood group typing by using acousto-optical technique in both cases of using isohemagglutinating sera as well as the crossmatch test in a short time and high efficiency compared with the traditional methods.

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“…It was for the first time when the authors were involved into the problem of automatic device creation for blood typing based on the use of a probing optical beam and stimulating ultrasonic radiation simultaneously in 1986 [13]. In the further studies and developments this method was carried out in analog [18][19][20] or digital forms [21][22][23][24][25][26][27][28][29] and in the whole it was named as "acousto-optical method for blood group determination".…”

Section: Introductionmentioning

confidence: 99%

The Sedimentation Rate of Free Erythrocytes and Their Associates, the Influence of a Standing Ultrasonic Wave

Doubrovski

Markov

Torbin

et al. 2021

J-BPE

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The Acousto-Optical Method for Blood Typing (Part 1). Photometric and Statistical Methods for Image Processing

Cited by 7 publications

References 8 publications

Digital Processing Techniques Influence on Resolution of Acousto-Optical Blood Typing Method

Digital Processing Techniques Influence on Resolution of Acousto-Optical Blood Typing Method

Enhancement the Agglutination of Erythrocytes in Blood Typing Test by Acousto-Optic Technique

The Sedimentation Rate of Free Erythrocytes and Their Associates, the Influence of a Standing Ultrasonic Wave

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