The actin‐binding protein Adseverin mediates neutrophil polarization and migration

Glogauer, Judah; Sun, Cong; Wang, Yongqiang; Glogauer, Michael

doi:10.1002/cm.21684

Cited by 4 publications

(2 citation statements)

References 22 publications

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“… 34 When looking more specifically at neutrophils, SCIN has been shown to be key for the migration as well as polarization of neutrophils. 35 The increased expression of SCIN in fxH samples of the ETC group aligns with the increased local inflammatory load after ETC. Regarding bone formation, SCIN is important for both chondrocyte maturation and the maintenance of chondrocyte phenotype, indicating potential implications for endochondral ossification.…”

Section: Discussionmentioning

confidence: 74%

Fracture haematoma proteomics

Groven,

Kuik,

Greven

et al. 2024

Bone Joint Res

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AimsThe aim of this study was to determine the fracture haematoma (fxH) proteome after multiple trauma using label-free proteomics, comparing two different fracture treatment strategies.MethodsA porcine multiple trauma model was used in which two fracture treatment strategies were compared: early total care (ETC) and damage control orthopaedics (DCO). fxH was harvested and analyzed using liquid chromatography-tandem mass spectrometry. Per group, discriminating proteins were identified and protein interaction analyses were performed to further elucidate key biomolecular pathways in the early fracture healing phase.ResultsThe early fxH proteome was characterized by immunomodulatory and osteogenic proteins, and proteins involved in the coagulation cascade. Treatment-specific proteome alterations were observed. The fxH proteome of the ETC group showed increased expression of pro-inflammatory proteins related to, among others, activation of the complement system, neutrophil functioning, and macrophage activation, while showing decreased expression of proteins related to osteogenesis and tissue remodelling. Conversely, the fxH proteome of the DCO group contained various upregulated or exclusively detected proteins related to tissue regeneration and remodelling, and proteins related to anti-inflammatory and osteogenic processes.ConclusionThe early fxH proteome of the ETC group was characterized by the expression of immunomodulatory, mainly pro-inflammatory, proteins, whereas the early fxH proteome of the DCO group was more regenerative and osteogenic in nature. These findings match clinical observations, in which enhanced surgical trauma after multiple trauma causes dysbalanced inflammation, potentially leading to reduced tissue regeneration, and gained insights into regulatory mechanisms of fracture healing after severe trauma.Cite this article: Bone Joint Res 2024;13(5):214–225.

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Section: Discussionmentioning

confidence: 74%

Fracture haematoma proteomics

Groven,

Kuik,

Greven

et al. 2024

Bone Joint Res

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“…Some critical examples of these proteins are Gelsolin, Galectin, Ezrin, and Elastase that we found in the ZF-treated plasma proteome. Gelsolin and Galectin play an immunomodulatory role in regulating the general PMN leukocytes movement, particularly neutrophil recruitment ( 94 , 95 ). At the same time, Ezrin and Elastase regulate cell adhesion by connecting critical membrane adhesion receptors, such as ICAM-I and VCAM, to the keratin or actin-based cytoskeleton in epithelial and endothelial tissues ( 96 , 97 ).…”

Section: Resultsmentioning

confidence: 99%

Plasma proteome responses in zebrafish following λ-carrageenan-Induced inflammation are mediated by PMN leukocytes and correlate highly with their human counterparts

et al. 2022

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Regulation of inflammation is a critical process for maintaining physiological homeostasis. The λ-carrageenan (λ-CGN) is a mucopolysaccharide extracted from the cell wall of red algae (Chondrus crispus) capable of inducing acute intestinal inflammation, which is translated into the production of acute phase reactants secreted into the blood circulation. However, the associated mechanisms in vertebrates are not well understood. Here, we investigated the crucial factors behind the inflammatory milieu of λ-CGN-mediated inflammation administered at 0, 1.75, and 3.5% (v/w) by i.p. injection into the peritoneal cavity of adult zebrafish (ZF) (Danio rerio). We found that polymorphonuclear leukocytes (neutrophils) and lymphocytes infiltrating the ZF peritoneal cavity had short-term persistence. Nevertheless, they generate a strong pattern of inflammation that affects systemically and is enough to produce edema in the cavity. Consistent with these findings, cell infiltration, which causes notable tissue changes, resulted in the overexpression of several acute inflammatory markers at the protein level. Using reversed-phase high-performance liquid chromatography followed by a hybrid linear ion-trap mass spectrometry shotgun proteomic approach, we identified 2938 plasma proteins among the animals injected with PBS and 3.5% λ-CGN. First, the bioinformatic analysis revealed the composition of the plasma proteome. Interestingly, 72 commonly expressed proteins were recorded among the treated and control groups, but, surprisingly, 2830 novel proteins were differentially expressed exclusively in the λ-CGN-induced group. Furthermore, from the commonly expressed proteins, compared to the control group 62 proteins got a significant (p < 0.05) upregulation in the λ-CGN-treated group, while the remaining ten proteins were downregulated. Next, we obtained the major protein-protein interaction networks between hub protein clusters in the blood plasma of the λ-CGN induced group. Moreover, to understand the molecular underpinnings of these effects based on the unveiled protein sets, we performed a bioinformatic structural similarity analysis and generated overlapping 3D reconstructions between ZF and humans during acute inflammation. Biological pathway analysis pointed to the activation and abundance of diverse classical immune and acute phase reactants, several catalytic enzymes, and varied proteins supporting the immune response. Together, this information can be used for testing and finding novel pharmacological targets to treat human intestinal inflammatory diseases.

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The role of actin cytoskeleton CFL1 and ADF/cofilin superfamily in inflammatory response

Xing,

Wang,

Peng

et al. 2024

Front. Mol. Biosci.

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Actin remodeling proteins are important in immune diseases and regulate cell cytoskeletal responses. These responses play a pivotal role in maintaining the delicate balance of biological events, protecting against acute or chronic inflammation in a range of diseases. Cofilin (CFL) and actin depolymerization factor (ADF) are potent actin-binding proteins that cut and depolymerize actin filaments to generate actin cytoskeleton dynamics. Although the molecular mechanism by which actin induces actin cytoskeletal reconstitution has been studied for decades, the regulation of actin in the inflammatory process has only recently become apparent. In this paper, the functions of the actin cytoskeleton and ADF/cofilin superfamily members are briefly introduced, and then focus on the role of CFL1 in inflammatory response.

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The actin‐binding protein Adseverin mediates neutrophil polarization and migration

Cited by 4 publications

References 22 publications

Fracture haematoma proteomics

Fracture haematoma proteomics

Plasma proteome responses in zebrafish following λ-carrageenan-Induced inflammation are mediated by PMN leukocytes and correlate highly with their human counterparts

The role of actin cytoskeleton CFL1 and ADF/cofilin superfamily in inflammatory response

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