The additive memory and healthspan enhancement effects by the combined treatment of mature silkworm powders and Korean angelica extracts

Nguyen, Phuong; Kim, Kee‐Young; Kim, A‐Young; Kang, SangKook; Osabutey, Angelica F.; Jin, Hui; Guo, Yanxia; Park, Hyun-Woo; Suh, Joo‐Won; Koh, Young Ho

doi:10.1016/j.jep.2021.114520

Cited by 11 publications

(7 citation statements)

References 45 publications

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“…In previous studies, we showed that Drosophila and mice fed GS showed enhanced mitochondrial function ( 11 , 12 ). Thus, we investigated whether the activities of MitoComs I∼IV in Drosophila reared with GSf or GSDf were different from those of Nf-reared Drosophila .…”

Section: Resultsmentioning

confidence: 96%

“…The activities of mitochondrial complexes (MitoCom) I∼IV in 5-, 10-, 15-, or 20-day-old Drosophila reared with Nf, GSf, or GSDf were measured as previously published ( 11 , 12 ). Ten Drosophila adults were ground in mitochondria lysis buffer (0.25 M sucrose, 5 mM Tris-HCl, 2 mM EGTA, and 1% BSA, pH 7.4), and then the debris was removed by three layers of medical gauze (Dae Han Medical Supply Co. LTD, Chuncheon-si, South Korea).…”

Section: Methodsmentioning

confidence: 99%

“…For comparing mitochondrial activities, amounts of phytochemicals, and antioxidant activities, one-way analysis of variance (ANOVA) and Tukey’s honestly significant difference (HSD) post-hoc analysis were performed using Microsoft Excel for Windows 10 (Microsoft, Redmond, WA, United States) as previously published ( 11 , 12 ). All results were presented as the mean ± the standard error of the mean.…”

Section: Methodsmentioning

confidence: 99%

“…In addition, Cox proportional hazard regression analyses and the log-rank tests were performed to obtain hazard ratios (HRs) and 95% confidential intervals (CIs), and p -values, respectively. Kaplan-Meier survival estimations, the log-rank tests, and Cox proportional hazard regression analyses were performed using the R program (version 4.0.3; The R Foundation) as previously published ( 12 , 37 ).…”

Section: Methodsmentioning

confidence: 99%

“…Therefore, mature silkworms must have various health improvement effects originating from their diverse functional nutrients ( 2 ). In fact, steamed and freeze-dried mature silkworms, also known as HongJam, have been reported to have memory enhancements in mild cognitive impairment rodent models ( 11 , 12 ), preventing the onset of rotenone-induced loss of motor control ( 13 – 15 ), gastrointestinal protection ( 16 , 17 ), liver function improvement ( 18 ), skin whitening ( 19 ), and promotion of the lifespan and healthspan ( 10 , 13 , 14 ).…”

Section: Introductionmentioning

confidence: 99%

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An Alkaline Protease-Digestion of Silkworm Powder Enhances Its Effects Over Healthspan, Autophagy, and Mitochondria Function in a Rotenone-Induced Drosophila Model

Xuan

Kang

et al. 2022

Front. Nutr.

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BackgroundRecent studies have reported that steamed and freeze-dried mature silkworms, also known as HongJam, have various health-promoting effects.ObjectiveThe goal of this study was to elucidate changes in the various health-promoting effects of HongJam, after its digestion with a food-grade protease.Materials and MethodsWe examined whether healthspan-promotion and rotenone-induced loss of motor-control prevention effects were enhanced in Drosophila fed with food-grade alkaline protease-digested HongJam compared to those fed with non-digested HongJam. The differences in mitochondrial functions, chemical susceptibilities, and activations of signal transduction pathways between Drosophila supplemented with various feed were examined to elucidate the molecular and biochemical basis of healthspan-promotion and locomotor-improvement effects of protease-digested HongJam.ResultsWe first found that the healthspan-promotion effect of HongJam digested with a food-grade protease was different depending on the silkworm variety used for its production. Digestion with food-grade protease into White-Jade HongJam (WJ) as prepared from the White-Jade silkworm variety that spins white cocoons did not enhance its functionality. However, compared to Golden-Silk HongJam (GS), a food-grade protease-digested Golden-Silk HongJam (GSD) produced from the Golden-Silk silkworm variety that spins yellow cocoons, it further promoted the healthspan in a Drosophila model. By conducting a series of studies to reveal the molecular and biochemical basis for healthspan-promoting effects, we found that GS and GSD similarly enhanced mitochondrial activity, but GSD activated autophagy signaling more than GS. In addition, GSD feed (GSDf)-, GSD supernatant feed (GSDsupf)-, and GSD precipitate feed (GSDprecf)-reared Drosophila were also found to have increased resistance to an autophagy inhibitor compared to that of normal feed- or GS feed-reared Drosophila. Furthermore, we found that the rotenone-induced loss of motor control prevention effect was superior for GSDsup compared to GS, GSD, or GSDprec. This result may have occurred because GSDsup has more phenolic compounds and antioxidant activities than other samples.ConclusionGSDsup contained more digested small peptides and free phytochemicals than other samples due to the digestion of proteins with a food-grade protease. Thus, GSDsup leads to further healthspan-promoting and locomotor-improvement effects than GS, GSD, or GSDprec.

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Section: Resultsmentioning

confidence: 96%

Section: Methodsmentioning

confidence: 99%