The adipocyte fatty acid-binding protein locus: characterization and association with intramuscular fat content in pigs

Gerbens, Frans; Jansen, A.P.; Erp, A.A.M. van; Harders, Frank; Meuwissen, T.H.E.; Rettenberger, G.; Veerkamp, J.H.; Pas, Marinus Fw Te

doi:10.1007/s003359900918

Cited by 121 publications

(87 citation statements)

References 24 publications

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“…No obvious positional candidate locus has so far been identified for FAT1. Gerbens et al [17] localised the adipocyte fatty acid-binding protein (A-FABP) gene on SSC 4 and suggested it might control fat distribution in pigs. Yet, further studies [18] did not support this hypothesis.…”

Section: Discussionmentioning

confidence: 99%

Detection of�quantitative trait loci for�carcass composition traits in pigs

et al. 2002

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-A quantitative trait locus (QTL) analysis of carcass composition data from a threegeneration experimental cross between Meishan (MS) and Large White (LW) pig breeds is presented. A total of 488 F2 males issued from six F1 boars and 23 F1 sows, the progeny of six LW boars and six MS sows, were slaughtered at approximately 80 kg live weight and were submitted to a standardised cutting of the carcass. Fifteen traits, i.e. dressing percentage, loin, ham, shoulder, belly, backfat, leaf fat, feet and head weights, two backfat thickness and one muscle depth measurements, ham + loin and back + leaf fat percentages and estimated carcass lean content were analysed. Animals were typed for a total of 137 markers covering the entire porcine genome. Analyses were performed using a line-cross (LC) regression method where founder lines were assumed to be fixed for different QTL alleles and a half/full sib (HFS) maximum likelihood method where allele substitution effects were estimated within each half-/full-sib family. Additional analyses were performed to search for multiple linked QTL and imprinting effects. Significant gene effects were evidenced for both leanness and fatness traits in the telomeric regions of SSC 1q and SSC 2p, on SSC 4, SSC 7 and SSC X. Additional significant QTL were identified for ham weight on SSC 5, for head weight on SSC 1 and SSC 7, for feet weight on SSC 7 and for dressing percentage on SSC X. LW alleles were associated with a higher lean content and a lower fat content of the carcass, except for the fatness trait on * Correspondence and reprints E-mail: milan@toulouse.inra.fr, bidanel@dga.jouy.inra.fr 706 D. Milan et al. SSC 7. Suggestive evidence of linked QTL on SSC 7 and of imprinting effects on SSC 6, SSC 7, SSC 9 and SSC 17 were also obtained. pig / gene mapping / quantitative trait locus / carcass composition

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Section: Discussionmentioning

confidence: 99%

Detection of�quantitative trait loci for�carcass composition traits in pigs

et al. 2002

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“…Genes involved in intracellular fatty-acid transport within skeletal muscles have been also notably proposed. Especially, studies put light on DNA markers or expression levels of FABP4 in pigs (Gerbens et al, 1998 andDamon et al, 2006), cattle Barendse et al, 2009) and avian species (Luo et al, 2006 in chicken;Saez et al, 2008 in ducks) to be associated to variations in IMF content. One study reported a specific allele of the PNAS-4 gene (i.e.…”

Section: Genetic Markersmentioning

confidence: 99%

Intramuscular fat content in meat-producing animals: development, genetic and nutritional control, and identification of putative markers

Hocquette

Gondret

Baéza³

et al. 2010

Animal

655

447

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Intramuscular fat (IMF) content plays a key role in various quality traits of meat. IMF content varies between species, between breeds and between muscle types in the same breed. Other factors are involved in the variation of IMF content in animals, including gender, age and feeding. Variability in IMF content is mainly linked to the number and size of intramuscular adipocytes. The accretion rate of IMF depends on the muscle growth rate. For instance, animals having a high muscularity with a high glycolytic activity display a reduced development of IMF. This suggests that muscle cells and adipocytes interplay during growth. In addition, early events that influence adipogenesis inside the muscle (i.e proliferation and differentiation of adipose cells, the connective structure embedding adipocytes) might be involved in interindividual differences in IMF content. Increasing muscularity will also dilute the final fat content of muscle. At the metabolic level, IMF content results from the balance between uptake, synthesis and degradation of triacylglycerols, which involve many metabolic pathways in both adipocytes and myofibres. Various experiments revealed an association between IMF level and the muscle content in adipocyte-type fatty acid-binding protein, the activities of oxidative enzymes, or the delta-6-desaturase level; however, other studies failed to confirm such relationships. This might be due to the importance of fatty acid fluxes that is likely to be responsible for variability in IMF content during the postnatal period rather than the control of one single pathway. This is evident in the muscle of most fish species in which triacylglycerol synthesis is almost zero. Genetic approaches for increasing IMF have been focused on live animal ultrasound to derive estimated breeding values. More recently, efforts have concentrated on discovering DNA markers that change the distribution of fat in the body (i.e. towards IMF at the expense of the carcass fatness). Thanks to the exhaustive nature of genomics (transcriptomics and proteomics), our knowledge on fat accumulation in muscles is now being underpinned. Metabolic specificities of intramuscular adipocytes have also been demonstrated, as compared to other depots. Nutritional manipulation of IMF independently from body fat depots has proved to be more difficult to achieve than genetic strategies to have lipid deposition dependent of adipose tissue location. In addition, the biological mechanisms that explain the variability of IMF content differ between genetic and nutritional factors. The nutritional regulation of IMF also differs between ruminants, monogastrics and fish due to their digestive and nutritional particularities.

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“…The PCR primers according to Gerbens et al (1998) (F: 5'GGG AAC TCT TGA AGT CTT TCT C, R: 5'GGT ACT TTC TGA TCT AAT GGT G) were used to amplify an approx. 250 bp fragment of the porcine A-FABP gene.…”

Section: Methodsmentioning

confidence: 99%

“…The pig A-FABP gene polymorphism was first described by Gerbens et al (1998). In the first intron three polymorphisms were found, namely a polymorphic microsatellite and two SNPs (single nucleotide polymorphisms), C/T and A/G substitutions occur within the microsatellite sequence.…”

Section: In: Adipocytes (A-fabp) the Liver (L-fabp) Intestine (Ffabmentioning

confidence: 99%

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Polymorphism of intronic microsatellites in the <i>A-FABP</i> and <i>LEPR</i> genes and its association with productive traits in the pig

Chmurzyńska¹,

Mackowski²,

Szydłowski³

et al. 2004

J. Anim. Feed Sci.

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The adipocyte fatty acid-binding protein {A-FABP) and leptin receptor (LEPR) genes are candidate quantitative trait loci (QTLs) for fatness traits in the pig. In both genes polymorphic short tandem repeat (STR) sequences in intron 1 (A-FABP) and in intron 3 (LEPR) were revealed. The association studies between both polymorphisms and productive traits were performed in two breeds (Polish Large White and Polish Landrace) and one synthetic line (L990) of the pig. In the A-FABP ten and in the LEPR gene eight alleles were identified, and the polymorphic information content (PIC) values ranged from 0.713 to 0.749 and from 0.441 to 0.557, respectively. The associations with backfat thickness and intramuscular fat content were found. Since the observed relationships considering each gene were not concordant in all the studied populations, it was concluded that both genes are linked with an unknown QTL.

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The adipocyte fatty acid-binding protein locus: characterization and association with intramuscular fat content in pigs

Cited by 121 publications

References 24 publications

Detection of�quantitative trait loci for�carcass composition traits in pigs

Detection of�quantitative trait loci for�carcass composition traits in pigs

Intramuscular fat content in meat-producing animals: development, genetic and nutritional control, and identification of putative markers

Polymorphism of intronic microsatellites in the <i>A-FABP</i> and <i>LEPR</i> genes and its association with productive traits in the pig

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