The adipocyte precursor cell

Van, Robin L. R.

doi:10.1016/b978-0-408-10857-7.50020-8

Cited by 16 publications

(14 citation statements)

References 69 publications

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“…i Merge. Bar50 μm differentiation and to be stationary cells that have lost their proliferative ability (Johnson and Goldstein 1983;Van 1985). However, recent studies have shown that mature adipocytes dedifferentiate into fibroblast-like adipocytes in ceiling culture and proliferate actively (Sugihara et al 1986(Sugihara et al , 1987Shigematsu et al 1999;Zhang et al 2000;Justesen et al 2004;Tholpady et al 2005).…”

Section: Discussionmentioning

confidence: 95%

“…However, cell lines derived from the Swiss 3T3 cell line obtained from disaggregated 17-to 19-day-old Swiss 3T3 mouse embryos, in which white adipose tissue is not detected, often possess characteristics that differ from those of tissue preadipocytes (Smas and Sul 1995;Wolins et al 2006). Alternatively, primary preadipocytes are derived from the stromal-vascular fraction of adult adipose tissue; therefore, they may reflect the in vivo context and provide a suitable in vitro primary culture system for identifying possible regulators (Van 1985). Although 3T3 preadipocyte cell lines have been derived from mice, primary preadipocytes have been used from various other species (Van and Roncari 1982;Butterwith et al 1992;Surywan et al 1997;Cho et al 2006;Torio-Padron et al 2007).…”

Section: Introductionmentioning

confidence: 98%

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Establishment of a preadipocyte cell line derived from mature adipocytes of GFP transgenic mice and formation of adipose tissue

2008

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We established a preadipocyte cell line from mature adipocytes obtained from subcutaneous fat tissue of green fluorescent protein (GFP) transgenic mice. The floating top layer, containing mature adipocytes, was isolated from subcutaneous fat tissue by collagenase digestion and filtration. Fluorescence-activated cell sorting and microscopic analysis revealed that the floating cell fraction comprised a highly homogeneous adipocyte population with no adipose stromal-vascular cells. Isolated mature adipocytes dedifferentiated into fibroblast-like cells and actively proliferated in ceiling culture. In vitro studies showed that the cells could redifferentiate into mature adipocytes in an identical way to 3T3-L1 preadipocytes. No changes in the differentiation pattern were observed during the propagation of our cells. They were successfully maintained and differentiated for at least 22 passages. We named these cells dedifferentiated fat (DFAT-GFP) cells. When DFAT-GFP cells were implanted subcutaneously into C57BL/6N mice, they developed highly vascularized fat pads that morphologically resembled normal subcutaneous adipose tissue and consisted of GFP-positive cells; however, implanted 3T3-L1 cells did not have such an effect on the mice. We conclude that DFAT-GFP cells provide a model that should enable us to study the mechanisms of adipocyte differentiation and adipose tissue formation in vivo and in vitro.

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Section: Discussionmentioning

confidence: 95%

Section: Introductionmentioning

confidence: 98%

Establishment of a preadipocyte cell line derived from mature adipocytes of GFP transgenic mice and formation of adipose tissue

2008

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“…They replicated and differentiated into cells with the morphological and biochemical characteristics of fat cells and provided the first in vitro proof for the existence of stromal cells in rodent and human subcutaneous adipose tissues. All the details concerning the preliminary studies on rodent and adipocyte precursors up to 1985 have been reviewed (372). In vitro differentiation protocols have been developed to study differentiation of adipocyte precursors in the SVF of various species (i.e., rat, mouse, pig, cow, chicken, and human) and its regulation by hormones.…”

Section: Adipogenic Cells: Freshly Isolated Cells Primary Cell Linesmentioning

confidence: 99%

Historical perspectives in fat cell biology: the fat cell as a model for the investigation of hormonal and metabolic pathways

Lafontan

2012

American Journal of Physiology-Cell Physiology

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Lafontan M. Historical perspectives in fat cell biology: the fat cell as a model for the investigation of hormonal and metabolic pathways. Am J Physiol Cell Physiol 302: C327-C359, 2012. First published September 7, 2011; doi:10.1152/ajpcell.00168.2011.-For many years, there was little interest in the biochemistry or physiology of adipose tissue. It is now well recognized that adipocytes play an important dynamic role in metabolic regulation. They are able to sense metabolic states via their ability to perceive a large number of nervous and hormonal signals. They are also able to produce hormones, called adipokines, that affect nutrient intake, metabolism and energy expenditure. The report by Rodbell in 1964 that intact fat cells can be obtained by collagenase digestion of adipose tissue revolutionized studies on the hormonal regulation and metabolism of the fat cell. In the context of the advent of systems biology in the field of cell biology, the present seems an appropriate time to look back at the global contribution of the fat cell to cell biology knowledge. This review focuses on the very early approaches that used the fat cell as a tool to discover and understand various cellular mechanisms. Attention essentially focuses on the early investigations revealing the major contribution of mature fat cells and also fat cells originating from adipose cell lines to the discovery of major events related to hormone action (hormone receptors and transduction pathways involved in hormonal signaling) and mechanisms involved in metabolite processing (hexose uptake and uptake, storage, and efflux of fatty acids). Dormant preadipocytes exist in the stroma-vascular fraction of the adipose tissue of rodents and humans; cell culture systems have proven to be valuable models for the study of the processes involved in the formation of new fat cells. Finally, more recent insights into adipocyte secretion, a completely new role with major metabolic impact, are also briefly summarized. history; adipocytes; insulin action; 3T3-L1 cells; glucose transport; fatty acids; lipolysis; catecholamines; preadipocytes SINCE THE EARLIEST TIMES, attention has been directed toward fat-related questions. It is currently accepted that the ability to store fat in adipose tissue was a major advantage for survival during cold winters and periods of food shortage for our "primitive" hunter ancestors. However, in modern societies, with increased food availability and nutritional efficacy, excessive fat deposition is frequently associated with degenerative or metabolic diseases. As early as 1901, life insurance companies noted that excess weight, particularly around the abdomen, was linked to reduced life expectancy. The risk was confirmed in the seminal studies of Vague (366) in Marseilles, who established the importance of abdominal (central) obesity in conferring excess mortality. The health hazard of obesity is not considered in the present review, which, rather, focuses on adipose tissue and adipocytes.

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“…Hence, short-term primary cultures have mainly been performed [Butterwith et al, 1992;Surywan et al, 1997;Tomlinson et al, 2006]. Primary preadipocytes are derived from the stromalvascular fraction (SVF) of adult adipose tissue; therefore, they may reflect the in vivo context and provide a stable in vitro primary culture system for identifying possible regulators [Van, 1985]. Of note, primary preadipocytes are characterized by low proliferative capacity associated with rapid decline of differentiation ability with subculturing, thereby limiting their use as cellular models.…”

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confidence: 99%

Establishment and characteristics of porcine preadipocyte cell lines derived from mature adipocytes

Nobusue

Kano

2009

J of Cellular Biochemistry

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Development of established preadipocyte cell lines, such as 3T3-L1 and 3T3-F442A, greatly facilitated the study of molecular mechanisms of adipocyte differentiation under defined conditions. Most of these cell lines are derived from mouse embryos, and preadipocyte cell lines of other species have not yet been maintained in culture long enough to study differentiation under a variety of conditions. This is the first report on the establishment of porcine preadipocyte cell lines derived from mature adipocytes by a simple method, known as ceiling culture, for culturing mature adipocytes in vitro. This cell line can proliferate extensively until the cells become confluent and fully differentiated into mature adipocytes, depending on adipogenic induction. No changes in their differentiation pattern are observed during their propagation, and they have been successfully carried and differentiated for at least 37 passages. This cell line maintains a normal phenotype without transforming spontaneously, even after long-term maintenance in culture. This achievement may lead to easy establishment of porcine preadipocyte cell lines and novel model systems for studying the mechanisms of adipocyte differentiation and metabolism as a substitute for human preadipocytes.

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The adipocyte precursor cell

Cited by 16 publications

References 69 publications

Establishment of a preadipocyte cell line derived from mature adipocytes of GFP transgenic mice and formation of adipose tissue

Establishment of a preadipocyte cell line derived from mature adipocytes of GFP transgenic mice and formation of adipose tissue

Historical perspectives in fat cell biology: the fat cell as a model for the investigation of hormonal and metabolic pathways

Establishment and characteristics of porcine preadipocyte cell lines derived from mature adipocytes

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