The advances of electromigration techniques applied for alkaloid analysis

Dziomba, Szymon; Belka, Mariusz; Kowalski, Piotr; Plenis, Alina; Bączek, Tomasz

doi:10.1002/bmc.2967

Cited by 5 publications

(3 citation statements)

References 145 publications

(273 reference statements)

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“…The great suitability of CE for alkaloids analysis was also highlighted in a comprehensive review by Dziomba et al. . Due to these reports, alkaloids were selected as the model analytes for the present study.…”

Section: Methodsmentioning

confidence: 89%

Evaluation of sample injection precision in respect to sensitivity in capillary electrophoresis using various injection modes

et al. 2017

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A comparative study was conducted to assess the injection precision in capillary electrophoresis for cationic analytes (arecoline, codeine, papaverine). The precision was measured in respect to methods sensitivity in various injection modes in capillary electrophoresis: standard hydrodynamic injection (3.45 kPa for 6 s), large volume sample stacking (3.45 kPa for 40 s), and field-amplified sample injection (10 kV for 65 s). All measurements were conducted for aqueous solutions of standards to minimize the errors linked to the sample preparation step. The methods were submitted to precision assessment at three concentration levels: at the limit of quantification, three-fold and ten-fold of limit of quantification. The results were compared to those from high-performance liquid chromatography as a reference technique. The field-amplified sample injection method was shown to provide greatest sensitivity (quantification limits down to 4 ng/mL for all three tested compounds) but the lowest precision. High-performance liquid chromatography was established as the most reliable technique (coefficient of variation in all intraday experiments was below 1%). It was also shown that with a use of large volume sample injection technique, similar sensitivity as in high-performance liquid chromatography can be easily reached.

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Section: Methodsmentioning

confidence: 89%

Evaluation of sample injection precision in respect to sensitivity in capillary electrophoresis using various injection modes

et al. 2017

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“…It is apparent from these works that central detection is not “required” for DOI‐CE separations as sometimes suggested in the literature . Table summarizes the migration distances utilized in all DOI‐CE experiments.…”

Section: Common Misconceptions Regarding Doi‐cementioning

confidence: 99%

Dual‐opposite injection capillary electrophoresis: Principles and misconceptions

Blackney

Foley

2016

Electrophoresis

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Dual-opposite injection capillary electrophoresis (DOI-CE) is a separation technique that utilizes both ends of the capillary for sample introduction. The electroosmotic flow (EOF) is suppressed to allow all ions to reach the detector quickly. Depending on the individual electrophoretic mobilities of the analytes of interest and the effective length that each analyte travels to the detection window, the elution order of analytes in a DOI-CE separation can vary widely. This review discusses the principles, applications, and limitations of dual-opposite injection capillary electrophoresis. Common misconceptions regarding DOI-CE are clarified.

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“…Sample stacking was also part of general reviews about micellar electrokinetic chromatography (MEKC) and MEEKC . Specialized reviews were published about micelle to solvent stacking (MSS) and large‐volume sample stacking (LVSS) with an EOF pump and stacking was also treated in reviews about electrophoresis of some groups of substances such as amino acids , alkaloids , and agrochemical residues . Stacking by transient isotachophoresis is not included in this review as it is part of another paper in this issue .…”

Section: Introductionmentioning

confidence: 99%

Contemporary sample stacking in analytical electrophoresis

et al. 2014

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This contribution is a methodological review of the publications about the topic from the last 2 years. Therefore, it is primarily organized according to the methods and procedures used in surveyed papers and the origin and type of sample and specification of analytes form the secondary structure. The introductory part about navigation in the architecture of stacking brings a brief characterization of the various stacking methods, with the description of mutual links to each other and important differences among them. The main body of the article brings a survey of publications organized according to main principles of stacking and then according to the origin and type of the sample. Provided that the paper cited gave explicitly the relevant data, information about the BGE(s) used, procedure, detector employed, and reached LOD and/or concentration effect is given. The papers where the procedure used is a combination of diverse fragments and parts of various stacking techniques are mentioned in a special section on combined techniques. The concluding remarks in the final part of the review evaluate present state of art and the trends of sample stacking in CE.

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The advances of electromigration techniques applied for alkaloid analysis

Cited by 5 publications

References 145 publications

Evaluation of sample injection precision in respect to sensitivity in capillary electrophoresis using various injection modes

Evaluation of sample injection precision in respect to sensitivity in capillary electrophoresis using various injection modes

Dual‐opposite injection capillary electrophoresis: Principles and misconceptions

Contemporary sample stacking in analytical electrophoresis

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