The alternative sigma factor SigB of Corynebacterium glutamicum modulates global gene expression during transition from exponential growth to stationary phase

Abstract

Background: Corynebacterium glutamicum is a gram-positive soil bacterium widely used for the industrial production of amino acids. There is great interest in the examination of the molecular mechanism of transcription control. One of these control mechanisms are sigma factors. C. glutamicum ATCC 13032 has seven putative sigma factor-encoding genes, including sigA and sigB. The sigA gene encodes the essential primary sigma factor of C. glutamicum and is responsible for promoter recognition of house-keeping genes. The sigB gene codes for the non-essential sigma factor SigB that has a proposed role in stress reponse.

Transcriptional regulation of gene expression inCorynebacterium glutamicum: the role of global, master and local regulators in the modular and hierarchical gene regulatory network

“…The primary or principal sigma factor SigA of C. glutamicum is believed to be essential and responsible for the transcription of the majority of the housekeeping genes (Oguiza et al, 1996). Expression of the sigA gene is transcriptionally regulated as a function of the growth phase of C. glutamicum as its transcription ceases when the cells reach the transition phase or the stationary phase (Oguiza et al, 1997;Larisch et al, 2007). The highest levels of sigA gene expression were detected by Northern hybridizations and RT-PCR assays during the exponential growth phase.…”

“…The nonessential primary-like sigma factor SigB of C. glutamicum is involved in responses to environmental , cg1143-cg1144, cg1735, cg1753, cg2361, cg2953, cg2965, cg2966, cg3220-cg3221, cg3226-lldD, cg3388, accD1, accDA, acn-acnR-gat, aceA, aceB, actA,adhA, ald, amtB-glnK-glnD, benABCD, benK, birA, catA, coaA-cg1139-cg1140-cg1141, ctaA, ctaC, ctaD, ctaEF, fabG1-cg0345-fadE-cg0347, fas-IA, fas-IB, fruR-pfkB1-ptsF, fum, gapA-tpi-pgk, gdh, genDFN, genR, genKH, glnA, gltA-fkb, gltBD, gluABCD, glxR, gntK, gntP, ilvBNC, ldh, ltsA, mptB-cg1767-cg1768, narKGHJI-arnR, ndh, nlpC-qcrBAC, nrdF, pcaHGBC, pck, pfkA, pobA-pcaK, prpD1B1C1, pstSCAB, ptsG, ptsI, putP, pyc, ramB, recJ, rhcHM1D1, rpf1, rpf2-ksgA-cg1039-cg1040-pdxK, sdhCDAB-cg0448,serC, sucCD, tctCBA, ugpAEBC, urtABCDE, uspA2, vanABK, whcA, whcE- stress factors and in gene expression during the transition phase and under oxygen deprivation (Halgasova et al, 2001;Larisch et al, 2007;Ehira et al, 2008). A disruption of the sigB gene affected the growth and viability of the C. glutamicum mutant under acid, salt, alcohol, heat and cold stress conditions, suggesting that SigB has a prominent role during not only the vegetative growth of C. glutamicum but also in the responses to various environmental stresses (Halgasova et al, 2002).…”

Growth and reducing capacity of Listeria monocytogenes under different initial redox potential

“…typhimurium, which is required for entry into and maintenance of stationary phase of growth in Gram negative bacteria. Related gram positive firmicutes like L. monocytogenes have alternative sigma factors to Rpo S such as r B (Larisch et al 2007). r B activates expression of numerous genes under environmental stress conditions and upon entry into stationary phase (Schwab et al 2005).…”

Enhancement of Microbial Biodesulfurization via Genetic Engineering and Adaptive Evolution

“…glutamicum and M . tuberculosis the SigB sigma factor plays a similar role [48,49]. In the experiments described here, limiting the sulfur source to a sparingly soluble molecule like DBT could be a signal of nutrient limitation.…”