The annulate lamellae—From obscurity to spotlight

Kessel, Richard G.

doi:10.1016/0892-0354(89)90003-8

Cited by 55 publications

(52 citation statements)

References 368 publications

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“…8, lanes 4' and 5'). Thc presence of p68 in the ooplasmic fraction might indicate the existence of a soluble cytoplasmic pool of p68 and/or rcflcct the occurrence of numerous pore complexes in cytoplasmic annulate lamellae (for referenccs see Kessel 1989). We conclude that the MT 42,000 cytoplasmic protcin recognized by antibody PI1 in immunoblots is not a component of nuclear pore complexes.…”

Section: P68 Occurs In Theform Of a Stable Supramolecular Complexmentioning

confidence: 79%

“…However, pore complcxes were not restricted to the nuclear envelopes of newly assembled nuclei but occurred also in the extranuclear cytosol in the form of annulate lamellae, either as single or stacked membrane cisternae (Fig. 3 b; for a detailed account of annulate lamellae see Kessel 1989). only one notable exception: the double-Iayered nuclear envelope consistently lacked pore complexes (Fig.…”

Section: Remova! Oj"proteins Binding To Antiho((1' Pli or Wga Fom Thementioning

confidence: 99%

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Identification of a soluble precursor complex essential for nuclear pore assembly in vitro

1990

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Abstract. We analysed the soluble form in which the nudear pore complex protein p68 is stored in Xenopus /acpis eggs and its involvement in pore complex assembly processes, We have shown previously that p68, which is the major wheat germ agglutinin (WGA)-binding glycoprotein 01' nudear pore complexes from Xcnopus 00-cytes, is located in the pore channel and partieipates in mediated transport 01' karyophilic proteins. Using a monodonal antibody direeted against p68 (PlI) we re11l0ved this protein from XCIlOPUS egg extract by immunoadsorption, On addition 01' lambda DNA the immunodepleted extraet supported reconstitution ofnudei which were surrounded by a continuous double-membrane envdope but lacked pore complexes and were unable to import karyophilic pro teins such as nudeoplasmin or lamin L m , Essentially identical results were obtained with extract depleted 01' WGA-binding proteins. Our finding that both the anti-p68 antibody and WGA efficiently removed components from the extraet neeessary lor pore complex assembly but did not interfere with nudear membrane formation demonstrates that these processes are independent 01' each other. Analysis 01' the immunoprecipitate on silver-stained SDS-polyaerylamide gels indicated that the antibody adsorbed other proteins besides p68, notably two high molecular weight components. By sucrose gradient centrifugation and gel Illtration we showed that p68 together with associated protein(s) forms a stable, approximately globular eomplex with an M, 01' 254,000, a Stokes radius 01' 5.2 nm and a sedimentation coelTicient 01' 11.3 S. Our tinding that p68 occurs in the form 01' larger maeromolecular assemblies offers an explanation for the distinetly punctale immunofluorescence pattern observed in the eytoplasm 01' mitotic cells after staining with antibodies to p68.

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Section: P68 Occurs In Theform Of a Stable Supramolecular Complexmentioning

confidence: 79%

Section: Remova! Oj"proteins Binding To Antiho((1' Pli or Wga Fom Thementioning

confidence: 99%

Identification of a soluble precursor complex essential for nuclear pore assembly in vitro

1990

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“…A review by Kessel (1989) meticulously summarized reports on the occurrences of AL in a variety of species and cell types, including observations related to pathological stages. Accordingly, AL were found in eukaryotes as different as mollusks, crustaceans, insects, amphibians, fish, reptiles, birds, plants and mammals.…”

Section: Occurrence and Affecting Factorsmentioning

confidence: 99%

“…Up to the 1990s a tremendous amount of EM data was accumulated, either as short 'case reports' or in the form of systematic studies, which all provided evidence for the existence of AL in a wide variety of eukaryotic cell types and species (Kessel, 1989). Despite their indicated omnipresence, AL were rarely encountered in TEM thin sections.…”

Section: The Annulate Lamellae Pore Complex: a Structure On The Searcmentioning

confidence: 99%

“…3 representing a surface view on AL by scanning electron microscopy. Kessel (1989) described such an optimal use of membrane space by ALPCs as a tight hexagonal package. Notably, NPCs may sometimes reach similarly high densities by clustering of pores at the NE, as observed for nuclei of the Xenopus oocytes at developmental stage 2-3 (Kiseleva, unpublished results), in growing blastoderm nuclei in drosophila embryo (Gubanova & Kiseleva, 2008), and in hematopoietic cells under pathological conditions leading to cell death (Reipert et al, 1996).…”

Section: Al Structure and Arrangementmentioning

confidence: 99%

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