The antibiotic activity and mechanisms of active metabolites (Streptomyces alboflavus TD-1) against Ralstonia solanacearum

Xue, Yibin; Yang, Mingguan; Li, Shuhua; Li, Zhenjing; Liu, Huanhuan; Guo, Qingbin; Wang, Changlu

doi:10.1007/s10529-019-02726-x

Cited by 7 publications

(4 citation statements)

References 31 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…When the cell wall is destroyed, AKP penetrates the cell [27]. Previous studies have shown that after treatment with antibacterial active substances, the permeability of bacteria increases, leading to the leakage of intracellular substances [28,29]. In this experiment, after aseptic filtrate (AF) was added, the OD 600 of the bacterial suspension decreased, and the relative conductivity, AKP, and nucleic acid content in the supernatant culture medium also showed an abnormal upward trend, which is consistent with the above conclusions.…”

Section: Discussionmentioning

confidence: 99%

Assessment of the Biocontrol Potential of Bacillus velezensis WL–23 against Kiwifruit Canker Caused by Pseudomonas syringae pv. actinidiae

Wang

Guo

Chen

et al. 2023

IJMS

View full text Add to dashboard Cite

Kiwifruit canker disease, caused by Pseudomonas syringae pv. actinidiae (Psa), is the main threat to kiwifruit production worldwide. Currently, there is no safe and effective disease prevention method; therefore, biological control technologies are being explored for Psa. In this study, Bacillus velezensis WL–23 was isolated from the leaf microbial community of kiwifruit and used to control kiwifruit cankers. Indoor confrontation experiments showed that both WL–23 and its aseptic filtrate had excellent inhibitory activity against the main fungal and bacterial pathogens of kiwifruit. Changes in OD600, relative conductivity, alkaline proteinase, and nucleic acid content were recorded during Psa growth after treatment with the aseptic filtrate, showing that Psa proliferation was inhibited and the integrity of the cell membrane was destroyed; this was further verified using scanning electron microscopy and transmission electron microscopy. In vivo, WL–23 promoted plant growth, increased plant antioxidant enzyme activity, and reduced canker incidence. Therefore, WL–23 is expected to become a biological control agent due to its great potential to contribute to sustainable agriculture.

show abstract

Section: Discussionmentioning

confidence: 99%

Assessment of the Biocontrol Potential of Bacillus velezensis WL–23 against Kiwifruit Canker Caused by Pseudomonas syringae pv. actinidiae

Wang

Guo

Chen

et al. 2023

IJMS

View full text Add to dashboard Cite

show abstract

“…The effects of CA on the morphology of Y. enterocolitica and E. sakazakii were determined using FEGSEM [41]. The preparation of the bacterial suspension was carried out according to Section 2.2.…”

Section: Field Emission Gun Scanning Electron Microscope Analysismentioning

confidence: 99%

Evaluation of the Membrane Damage Mechanism of Chlorogenic Acid against Yersinia enterocolitica and Enterobacter sakazakii and Its Application in the Preservation of Raw Pork and Skim Milk

Tian

Guo

et al. 2021

Molecules

View full text Add to dashboard Cite

Plant-derived antimicrobial agents have adequate antimicrobial effects on food-borne pathogens, which can be used as food preservatives. The purpose of this study was to evaluate the antibacterial mechanism of chlorogenic acid (CA) against Yersinia enterocolitica and Enterobacter sakazakii. The minimum inhibitory concentration (MIC) of CA was determined by employing the broth microdilution method. Then, the cell function and morphological changes of Y. enterocolitica and E. sakazakii treated with CA were characterized. Finally, the growth inhibition models of Y. enterocolitica in raw pork and E. sakazakii in skim milk were constructed through the response surface methodology. The results demonstrated that CA has a satisfactory inhibitory effect against Y. enterocolitica and E. sakazakii with a MIC of 2.5 mg/mL. In addition, CA inhibited the growth of Y. enterocolitica and E. sakazakii via cell membrane damage, such as depolarization of the cell membrane, reduction in intracellular adenosine triphosphate (ATP) and pH levels, and destruction of cell morphology. Moreover, CA reduced two log cycles of Y. enterocolitica in raw pork and E. sakazakii in skim milk at a certain temperature. According to the corresponding findings, CA has the potential to be developed as an effective preservative to control Y. enterocolitica and E. sakazakii-associated foodborne diseases.

show abstract

“…The results showed that the structure of the S. aureus cell wall and membrane was changed by PSBE. Some studies have shown that when the integrity of the bacterial cell membrane was destroyed, the cell permeability increased and the cell content flowed out, which led to cell death (Xue et al 2019;Dai et al 2020). The antibacterial activity of plant extracts was mostly achieved by destroying the bacterial cell wall and membrane (de Souza et al 2020;Zhang et al 2020b).…”

Section: Ultrastructure Observation Of S Aureus By Sem Analysismentioning

confidence: 99%

Antioxidant and antibacterial activities of Pterocarya stenoptera bark extract and its mechanism on Staphylococcus aureus through cell membrane damage

Zhang

Xing

et al. 2021

BioRes

View full text Add to dashboard Cite

Pterocarya stenoptera bark extract (PSBE) was assessed for its chemical constituents, antioxidant activity, and antibacterial activity. The total phenolic content (TPC) in the PSBE was 272.92 ± 3.22 mg gallic acid equivalent (GAE)/g. Three phenolic compounds (quercetin-3-O-(2″-O-galloyl)-rhamnoside, quercetin, and juglone) were identified in the PSBE via ultraperformance liquid chromatography/mass spectroscopy (UPLC/MS) analysis. The PSBE possessed remarkable antioxidant activity in the DPPH and ABTS+ radicals scavenging system, with IC50 values of 96.25 ± 3.82 μg/mL and 158.26 ± 6.08 μg/mL, respectively. Moreover, the PSBE had high antibacterial activity, which was especially sensitive to Staphylococcus aureus. The antibacterial mechanism of the PSBE on the S. aureus was related to the deformation of the cell morphology and the destruction of the cell membrane structure, which was confirmed using a BacLight Viability Kit and scanning electron microscope (SEM) observations. These findings suggest that the strong antioxidant and antibacterial properties of the bark extract from P. stenoptera make the tree species suitable as a natural additive in the pharmaceutical, food, and cosmetic industries.

show abstract

The antibiotic activity and mechanisms of active metabolites (Streptomyces alboflavus TD-1) against Ralstonia solanacearum

Cited by 7 publications

References 31 publications

Assessment of the Biocontrol Potential of Bacillus velezensis WL–23 against Kiwifruit Canker Caused by Pseudomonas syringae pv. actinidiae

Assessment of the Biocontrol Potential of Bacillus velezensis WL–23 against Kiwifruit Canker Caused by Pseudomonas syringae pv. actinidiae

Evaluation of the Membrane Damage Mechanism of Chlorogenic Acid against Yersinia enterocolitica and Enterobacter sakazakii and Its Application in the Preservation of Raw Pork and Skim Milk

Antioxidant and antibacterial activities of Pterocarya stenoptera bark extract and its mechanism on Staphylococcus aureus through cell membrane damage

Contact Info

Product

Resources

About