The antimalarial agent artesunate causes sperm DNA damage and hepatic antioxidant defense in mice

Singh, Supriya; Giri, Anirudha; Giri, Sarbani

doi:10.1016/j.mrgentox.2014.11.001

Cited by 27 publications

(19 citation statements)

References 46 publications

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“…Recent reports have indicated concerns of reproductive toxicity by artemisinin drugs in animals (Obianime & Aprioku, , ; Raji, Osonuga, Akinsomisoye, Osonuga, & Mewoyeka, ; Singha, Giri, & Giri, ). Raji et al.…”

Section: Introductionmentioning

confidence: 99%

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Study on testicular response to prolong artemisinin-based combination therapy treatments in guinea pigs

Aprioku

Mankwe

2017

Andrologia

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Artemisinin-based combination therapies (ACTs) are first-line agents in malaria chemotherapy, but often abused in malaria endemic countries including Nigeria. This study investigated the effects of prolong treatment of artesunate-amodiaquine (ATS-Amod), artesunate-sulfadoxine-pyrimethamine (ATS-SP) and artemether-lumefantrine (ATM-Lum) on testicular indices in guinea pigs. Sixty-five pigs were grouped into 13 (n = 5 per group). Six groups were given standard or double therapeutic dose equivalents of ATS-Amod, ATS-SP or ATM-Lum daily for 14 day and sacrificed 24 hr after treatments. Six other groups (recovery groups) received similar drug treatments but allowed to recover for 14 day before sacrificed. Control group received distilled water. ATS-Amod, ATS-SP and ATM-Lum, respectively, decreased (p < .01) sperm count (17.7%, 37.7% and 33.8%), motility (48.6%, 50% and 51.4%), viability (32.7%, 43.7% and 35.9%) and morphology (123.5%, 0% and 0%), compared to control. These effects were reversed in recovery animals. Also, they decreased (p < .01) luteinising hormone and testosterone serum levels, without affecting follicle-stimulating hormone. Testicular malondialdehyde level was elevated, and glutathione was decreased, while catalase and superoxide dismutase enzymes were unaffected by the drugs. The alterations were all reversed in recovery animals. The study reveals that prolong administration of ACTs results in reversible alteration of sperm parameters and reduction of testosterone which is partly attributable to oxidative stress.

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Section: Introductionmentioning

confidence: 99%

“…Singha et al. () have reported that artesunate causes sperm DNA damage in mice. It has also been shown that treatment with standard dose levels of some ACTs and their combinants cause reductions in testosterone level (Obianime & Aprioku, ).…”

Section: Introductionmentioning

confidence: 99%

Study on testicular response to prolong artemisinin-based combination therapy treatments in guinea pigs

Aprioku

Mankwe

2017

Andrologia

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“…Nevertheless, % Tail DNA should be used for DNA damage quantification. Another limitation is that we performed the Comet Assay experiments on thawed embryos after an in vitro exposure to two well-known genotoxic agents; our protocol should also be tested to detect embryo DNA damage induced after in vivo mice exposure associated to DNA damage in gametes (Einaudi et al, 2014;Singh et al, 2015).…”

Section: Limitationsmentioning

confidence: 99%

Comet assay on thawed embryos: An optimized technique to evaluate DNA damage in mouse embryos

Rolland

Courbière

Tassistro

et al. 2017

Toxicology in Vitro

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1) The recovery rate was 3,3% (n=5/150) with the 2 agarose layers protocol and 71,3% (n=266/371) with the 3 agarose layers protocol. 2) DNA damage did not differ statistically significantly between ZP- and ZP+ embryos (12.60±2.53% Tail DNA vs 11.04±1.50 (p=0.583) for the control group and 49.23±4.16 vs 41.13±4.31 (p=0.182) for the HO group); 3) HO and SSI induced a statistically significant increase in DNA damage compared with the control group (41.13±4.31% Tail DNA, 36.33±3.02 and 11.04±1.50 (p<0.0001)). The CA on mammal embryos was optimized by using thawed embryos, by avoiding ZP removal and by the adjunction of a third agarose layer.

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“…The mechanism of action of ART is not well understood but a possible mechanism is postulated to involve the heme-mediated decomposition of the endo-peroxide bond to produce free radicals which kill the parasites accumulated in the erythrocytes [4]. Recent studies suggest that this medicine can induce toxicity to male germ cells which may have implications in male reproductive toxicity [5].…”

Section: Introductionmentioning

confidence: 99%

Electrochemical reduction of artemisinin: Chromatographic identification of bulk electrolysis products

Ombwayo

Mazzhichette

2019

J. Electrochem. Sci. Eng.

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<p class="PaperAbstract"><span lang="EN-US">Artemisinin is a naturally occurring sesquiterpene lactone with an endo-peroxide bond. This drug is used for treatment of many diseases including malaria. The reduction of this molecule on an electrode surface was carried out by cyclic voltammetry as well as amperometry. Cyclic voltammetry of artemisinin generated one prominent peak wave at -1.0 V and another, smaller one at -0.3 V vs Ag/AgCl reference electrode. The bulk electrolysis of artemisinin on a carbon electrode generated two other irreversible peak waves at around -0.7 and -0.1 V. The concentration of the products was dependent on the time of electrolysis. LC-MS was used to determine the bulk electrolysis products of artemisinin. Initially dihydroartemisinin was generated as the main reduction product. Other reduction products were formed after further reduction of dyhidroartemisinin.</span></p>

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The antimalarial agent artesunate causes sperm DNA damage and hepatic antioxidant defense in mice

Cited by 27 publications

References 46 publications

Study on testicular response to prolong artemisinin-based combination therapy treatments in guinea pigs

Study on testicular response to prolong artemisinin-based combination therapy treatments in guinea pigs

Comet assay on thawed embryos: An optimized technique to evaluate DNA damage in mouse embryos

Electrochemical reduction of artemisinin: Chromatographic identification of bulk electrolysis products

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