The antioxidative defense system is involved in the delayed senescence in a wheat mutant tasg1

Hui, Zhen; Tian, Fei; Wang, Guokun; Wang, Guiping; Wang, Wei

doi:10.1007/s00299-012-1226-z

Cited by 70 publications

(42 citation statements)

References 40 publications

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“…The results in this study combined with our previous observations , Hui et al 2012 suggest that a greater antioxidant competence may contribute to delayed senescence and to drought resistance of tasg1. These data are helpful for better understanding the mechanism of drought resistance in stay-green mutants.…”

Section: Discussionsupporting

confidence: 75%

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Antioxidative defence under drought stress in a wheat stay-green mutant

Tian¹,

Zhang²,

Wang³

et al. 2015

Biologia plant.

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A wheat stay-green mutant, named tasg1, was generated using the mutagen ethyl methane sulphonate applied to wheat (Triticum aestivum L.) cv. HS2. A drought stress was imposed by controlling irrigation and sheltering plants from rain. The antioxidant defence was characterized in the flag leaves of the tasg1 and wild-type (WT). Compared with WT, tasg1 had higher reduced ascorbate/oxidized ascorbate ratio, reduced glutathione/oxidized glutathione ratio, and antioxidant enzyme activities during senescence under both normal and drought stress conditions. The DHAR gene expression remained higher in tasg1 than in WT during the drought stress and tasg1 had a higher antioxidant defence competence which may contribute towards the delayed leaf senescence. The different transcriptional responses of some wheat senescence-associated genes to the drought stress between tasg1 and WT were observed. These results suggest that the competent antioxidative capacity might play an important role in the enhanced drought tolerance in tasg1.

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Section: Discussionsupporting

confidence: 75%

“…Tasg1 is a functional stay-green mutant with drought resistance characteristics according to our previous studies , Hui et al 2012). In the present work, the antioxidant defence mechanism and the expression of senescence-associated genes were analyzed.…”

Section: Introductionmentioning

confidence: 99%

Antioxidative defence under drought stress in a wheat stay-green mutant

Tian¹,

Zhang²,

Wang³

et al. 2015

Biologia plant.

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“…Therefore, we hypothesized that the contribution of TaNAM to ROS network through modulation of enzymatic activity may be probably related to an indirect effect derived from a global senescence syndrome more than to a primary effect related with ROS signalling, although the last possibility should not be completely discarded. Besides, it should be noted that evidence for a differential control of the antioxidant response in the 17 tasg1 stay-green T. aestivum mutant has been provided (Hui et al, 2012). In this mutant data has been also obtained for preservation of chloroplast ultrastructure .…”

Section: Discussionmentioning

confidence: 86%

“…On the other hand, whilst the involvement of reactive oxygen species (ROS) during plant senescence either as determinants of cell injury as well as in the signalling network has 5 been widely documented (Thompson et al, 1987;Prochazkova et al, 2001;KriegerLiszkay et al, 2015), only recently a possible link between the way that wheat plants handle ROS accumulation and the stay-green trait started to emerge (Hui et al, 2012;De Simone et al, 2014). …”

Section: Introductionmentioning

confidence: 99%

The stay-green phenotype of TaNAM-RNAi wheat plants is associated with maintenance of chloroplast structure and high enzymatic antioxidant activity

Checovich

Galatro

Moriconi

et al. 2016

Plant Physiology and Biochemistry

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SummaryTaNAM transcription factors play an important role in controlling senescence, which in turn, influences the delivery of nitrogen, iron and other elements to the grain of wheat (Triticum aestivum) plants, thus contributing to grain nutritional value. While lack or diminished expression of TaNAMs determines a stay-green phenotype, the precise effect of these factors on chloroplast structure has not been studied. In this work we focused on the events undergone by chloroplasts in two wheat lines having either control or diminished TaNAM expression due to RNA interference (RNAi). It was found that in RNAi plants maintenance of chlorophyll levels and maximal photochemical efficiency of photosystem II were associated with lack of chloroplast dismantling. Flow cytometer studies and electron microscope analysis showed that RNAi plants conserved organelle ultrastructure and complexity. It was also found that senescence in control plants was accompanied by a low leaf enzymatic antioxidant activity. Lack of chloroplast dismantling in RNAi plants was associated with maintenance of protein and iron concentration in the flag leaf, the opposite being observed in control plants. These data provide a structural basis for the observation that down regulation of TaNAMs confers a functional stay-green phenotype and indicate that the low export of iron and nitrogen from the flag leaf of these plants is concomitant, within the developmental window studied, with lack of chloroplast degradation and high enzymatic antioxidant activity. 3 Highlights•TaNAM-RNAi stay-green phenotype is coupled with maintenance of chloroplast structure.•That stay-green phenotype is associated with a high enzymatic antioxidant activity.•Chloroplast dismantling correlates with decay of iron and protein content in leaves.

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“…In stay-green maize, the delay of senescence was associated with higher CAT and SOD levels (He et al 2005). Hui et al (2012) suggest that the higher antioxidant defense system may be one of the most important mechanisms underlying the expression of the delayed senescence phenotype in staygreen wheat. In this study, staygreen wheat (Wennong 6) exhibited the higher SOD, POD, and CAT activity and lower MDA content than those in Jimai 20.…”

Section: Discussionmentioning

confidence: 97%

Application of abscisic acid regulates antioxidant enzymes activities and modulates endosperm cell division in winter wheat

et al. 2016

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Effects of exogenous abscisic acid (ABA) on antioxidant enzymes activities and endosperm cell division of two wheat (Triticum aestivum L.) cultivars were investigated. Results showed that the superoxide dismutase (SOD), peroxidase (POD), and catalase (CAT) activity in flag leaves of both cultivars is elevated by application of ABA and it is correlated with less membrane damage: lower malondialdehyde (MDA). Exogenous ABA significantly increased endosperm cell number and endosperm division rate, finally increased grain weight. Although ABA treatment decreased endogenous zeatin riboside (ZR) content in flag leaves from 7 to 28 days after anthesis (DAA), indole acetic acid (IAA) levels were significantly increased by spraying with ABA. Correlation analysis showed that endogenous contents of ZR, ABA, and IAA in grains were positively and significantly correlated with grain-filling rate. IAA content in leaves was positively and significantly correlated with grain-filling rate. The results suggested that increased grain weight of ABA-treated plants was due to higher antioxidant abilities of flag leaf resulting in longer maintenance of photosynthetic capacity and higher grain-filling rate.Key words: hormones, antioxidant enzymes, malondialdehyde, grain filling, staygreen, Triticum aestivum L.Résumé : Les auteurs ont examiné les effets de l'acide abscissique (AA) exogène sur l'activité des enzymes antioxydants et la division des cellules dans l'endosperme de deux cultivars de blé (Triticum aestivum L.). Les résultats indiquent que l'application d'AA augmente l'activité de la superoxyde dismutase (SOD), de la peroxydase (POD) et de la catalase (CAT) dans les feuilles paniculaires des deux variétés, et que cette hausse est corrélée à des dommages moins importants à la membrane cellulaire, à savoir une concentration plus faible de malondialdéhyde (MDA). L'AA exogène accroît le nombre et la multiplication des cellules dans l'endosperme de façon significative, ce qui a pour conséquence de donner un grain plus lourd. Bien que l'application d'AA diminue la concentration de zéatine riboside (ZR) endogène dans les feuilles paniculaires sept à 28 jours après l'anthèse, la pulvérisation d'AA augmente significativement la celle d'acide indole acétique (IAA). L'analyse des corrélations révèle que la teneur endogène en ZR, AA, et IAA du grain est positivement et significativement corrélée au remplissage du grain. La concentration d'IAA dans les feuilles présente une corrélation positive significative avec ce facteur. Les résultats donnent à penser que le poids plus élevé du grain des plants traités à l'AA est attribuable au meilleur pouvoir antioxydant de la feuille paniculaire, qui permet à la photosynthèse de se poursuivre plus longtemps et au grain de se remplir davantage. [Traduit par la Rédaction]

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The antioxidative defense system is involved in the delayed senescence in a wheat mutant tasg1

Cited by 70 publications

References 40 publications

Antioxidative defence under drought stress in a wheat stay-green mutant

Antioxidative defence under drought stress in a wheat stay-green mutant

The stay-green phenotype of TaNAM-RNAi wheat plants is associated with maintenance of chloroplast structure and high enzymatic antioxidant activity

Application of abscisic acid regulates antioxidant enzymes activities and modulates endosperm cell division in winter wheat

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