The Archaebacterium Thermofilum pendens Represents, a Novel Genus of the Thermophilic, Anaerobic Sulfur Respiring Thermoproteales

Zillig, Wolfram; Gierl, Alfons; Schreiber, G; Wunderl, Simon; Janekovic, D.; Stetter, Karl O.; Klenk, Hans Peter

doi:10.1016/s0723-2020(83)80035-6

Cited by 159 publications

(74 citation statements)

References 10 publications

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“…The G + C contents of DNAs of the three isolates are 52 mol% and clearly different from those of Thermoproteus species (Bonch-Osmolovskaya et al, 1990 ;Zillig et al, 1981; T. Itoh, K. Suzuki & T. Nakase, unpublished results) and ThermoJilum pendens (Zillig et al, 1983) which are in the range 56-60 mol%.…”

Section: Discussionmentioning

confidence: 71%

“…Two families, ThermoJilaceae and Thermoproteaceae, are known in this order. The former family contains only the genus ThermoJilum (Zillig et al, 1983), whereas the latter family comprises two genera, Pyrobaculum (Huber et al, 1987;Volkl et al, 1993) and Thermoproteus (Bonch-Osmolovskaya et al, 1990;Zillig, 1989;Zillig et al, 1981). Both genera are commonly encountered in continental solfataric areas, although Pyrobaculum aerophilum was isolated from a boiling marine waterhole (Volkl et al, 1993).…”

Section: Discussionmentioning

confidence: 99%

“…The archaeal cellextract solution stimulates growth of the new isolates. This preparation seems to be related to the growth factor required for Thermofilum pendens (Zillig et al, 1983). Unlike ThermoJilum pendens, growth of the isolates is enhanced by the cell-extract solution from Thermoplasrna or by a vitamin mixture (Balch et al, 1979).…”

Section: Discussionmentioning

confidence: 99%

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Thermocladium modestius gen. nov., sp. nov., a new genus of rod-shaped, extremely thermophilic crenarchaeote

Itoh

Suzuki

Nakase

1998

International Journal of Systematic Bacteriology

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Three strains of novel, extremely thermophilic, rod-shaped crenarchaeotes were isolated from acidic hot spring areas in Japan. Cells of the three strains were straight or slightly curved rods and occasionally branched out singly or elytensively, or had spherical bodies protruding a t the ends of the cells. They were heterotrophs that grew anaerobically or microaerobically. The presence of CO, in the gas phase, archaeal cell-extracts and a vitamin mixture stimulated growth of the strains. Growth occurred a t 45-82 "C and pH 2-6-5-9 and was optimal around 75 "C and pH 4.0. The strains utilized glycogen, starch, gelatin and various proteinaceous complex compounds as carbon sources. They required sulfur, thiosulfate or L-cystine as possible electron acceptors.The lipids mainly consisted of various cyclic glycerol-bisdiphytanyl-glycerol tetraethers. The G+C contents of the genomic DNAs were 52 molo/o. Comparison of the 16s rDNA sequences indicated that they belonged to a separate lineage in the family Thermoproteaceae. The three strains were included in a single species due to high levels of DNA-DNA hybridization values. Based upon these results, the new isolates were assigned to a new genus and species in the family Thermoproteaceae, Thermocladium modestius gen. nov., sp. nov. The type strain is Thermocladium modestius IC-125T (= JCM 100883.

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Section: Discussionmentioning

confidence: 71%

Section: Discussionmentioning

confidence: 99%

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Thermocladium modestius gen. nov., sp. nov., a new genus of rod-shaped, extremely thermophilic crenarchaeote

Itoh

Suzuki

Nakase

1998

International Journal of Systematic Bacteriology

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“…Thermoprotrus neutrophilus (DSM 2338) [7] was grown anaerobically on a mineral salts medium with H2 and sulfur as energy source and C 0 2 with acetate as sole carbon sources, as described previously [5]. [1-l3C]Acetate or [2-13C]acetate was added to the mineral growth medium in a concentration of 5 mM.…”

Section: Growth Conditionsmentioning

confidence: 99%

¹³C‐NMR study of acetate assimilation in Thermoproteus neutrophilus

Schäfer

Paalme²,

Vilu³

et al. 1989

European Journal of Biochemistry

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Acetate assimilation into amino acids and the functioning of central biosynthetic pathways in the extremely thermophilic anaerobic archaebacterium Thermoproteus neutrophilus was invesligated using 3C NMR as the method for determination of the labelling patterns. Acetate was assimilated via reductive carboxylation of acetyl-CoA to pyruvate and pyruvate conversion to phosphoenolpyruvate which was further carboxylated to oxaloacetate. 2-Oxoglutarate was mainly formed via citrate. However, the labelling patterns of glutaniic acid and alanine were in agreement with the concurrent synthesis of about 15% 2-oxoglutarate and 5% pyruvatc through the reductive citric acid cycle. A scrambling phenomenon occurring in aspartate and all amino acids derived through oxaloacetate was observed. The labelling patterns of amino acids were in agreement with their standard biosynthetic pathways, with two remarkable exceptions : isoleucine was synthesized via the citramalate pathway and lysine was synthesized via the 2-aminoadipate pathway which has previously been reported only in eukaryotic microorganisms.

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“…With the exception of the enzymes from Halobacterium (Zillig et al, 1978;, Halococcus (Madon et ai, 1983), Methanococcus, and Methanosarcina (Thomm, 1983), polymerases of archaebacteria were purified by a variation of the polymin P method originally used for the isolation of E. coli RNA polymerase (Zillig et al, 1919(Zillig et al, , 1983aSturm et ai, 1980;Prangishvilli et al, 1982).…”

Section: Purification Of Archaebacterial Rna Polymerasesmentioning

confidence: 99%

DNA-Dependent RNA Polymerases of the Archaebacteria

Zillig¹,

Stetter²,

Schnabel³

et al. 1985

Archabacteria

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The Archaebacterium Thermofilum pendens Represents, a Novel Genus of the Thermophilic, Anaerobic Sulfur Respiring Thermoproteales

Cited by 159 publications

References 10 publications

Thermocladium modestius gen. nov., sp. nov., a new genus of rod-shaped, extremely thermophilic crenarchaeote

Thermocladium modestius gen. nov., sp. nov., a new genus of rod-shaped, extremely thermophilic crenarchaeote

¹³C‐NMR study of acetate assimilation in Thermoproteus neutrophilus

DNA-Dependent RNA Polymerases of the Archaebacteria

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The Archaebacterium Thermofilum pendens Represents, a Novel Genus of the Thermophilic, Anaerobic Sulfur Respiring Thermoproteales

Cited by 159 publications

References 10 publications

Thermocladium modestius gen. nov., sp. nov., a new genus of rod-shaped, extremely thermophilic crenarchaeote

Thermocladium modestius gen. nov., sp. nov., a new genus of rod-shaped, extremely thermophilic crenarchaeote

13C‐NMR study of acetate assimilation in Thermoproteus neutrophilus

DNA-Dependent RNA Polymerases of the Archaebacteria

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¹³C‐NMR study of acetate assimilation in Thermoproteus neutrophilus