The ascorbate peroxidase regulated by H2O2 and ethylene is involved in cotton fiber cell elongation by modulating ROS homeostasis

Qin, Yong-Mei; Hu, Chunyang; Zhu, Yu-Xian

doi:10.4161/psb.3.3.5208

Cited by 60 publications

(50 citation statements)

References 24 publications

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“…Three important genes for ethylene biogenesis were reduced compared with the wild type. Previous studies demonstrated that ethylene and H 2 O 2 interact to regulate cotton fiber elongation, and they can be induced by each other Qin et al, 2008). However, the increase in H 2 O 2 content is accompanied by downregulation of the three ACO genes in the PDF1-RNAi cotton (Fig.…”

Section: The Potential Role Of Gbpdf1 In Cotton Fiber Developmentmentioning

confidence: 80%

GbPDF1 Is Involved in Cotton Fiber Initiation via the Core cis-Element HDZIP2ATATHB2

et al. 2011

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Cotton (Gossypium spp.) fiber cells are seed trichomes derived from the epidermal layer of the cotton seed coat. The molecular components responsible for regulating fiber cell differentiation have not been fully elucidated. A cotton PROTODERMAL FACTOR1 gene (GbPDF1) was found to be expressed preferentially during fiber initiation and early elongation, with highest accumulation in fiber cells 5 d post anthesis. PDF1 silencing caused retardation of fiber initiation and produced shorter fibers and lower lint percentage compared with the wild type, indicating that the gene is required for cotton fiber development. Further analysis showed that a higher accumulation of hydrogen peroxide occurred in the RNA interference transgenic cotton lines. Meanwhile, the expression of several genes related to ethylene and pectin synthesis or sugar transport during cotton fiber growth was found to be significantly reduced in the PDF1-suppressed cotton. Three proteins interacting with GbPDF1 in yeast and in planta might involve cellular signaling or metabolism. GbPDF1 promoter::GUS constructs in transgenic cotton were predominantly expressed in the epidermis of ovules and developing fibers. Progressive deletions of the GbPDF1 promoter showed that a 236-bp promoter fragment was sufficient for basal GbPDF1 transcription in cotton. Mutation of putative regulatory sequences showed that HDZIP2ATATHB2, an element within the fragment, was essential for PGbPDF1-1 expression. The binding activity between this cis-element and nuclear extracts from fiber-bearing cotton ovules at 5 d post anthesis was specific. We conclude that GbPDF1 plays a critical role together with interaction partners in hydrogen peroxide homeostasis and steady biosynthesis of ethylene and pectin during fiber development via the core cis-element HDZIP2ATATHB2.

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Section: The Potential Role Of Gbpdf1 In Cotton Fiber Developmentmentioning

confidence: 80%

GbPDF1 Is Involved in Cotton Fiber Initiation via the Core cis-Element HDZIP2ATATHB2

et al. 2011

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“…APX is a major H 2 O 2 -scavenging enzyme, and both cytosolic APX and stromal APX, which is targeted in the chloroplast and mitochondria, contribute to cellular redox homeostasis. Previous studies have shown that the cytosolic APX1 transcript and protein are enriched in fast elongating fibers [27,38], and stromal APX and cytosolic APX may function at different stages. Protein disulfide isomerase (PDI), which was preferentially expressed in fl ovules at 0 DPA (spot 105), can oxidize, reduce, and isomerize disulfide bonds, and can chaperone proteins.…”

Section: 3mentioning

confidence: 99%

Comparative proteomic analysis reveals the mechanisms governing cotton fiber differentiation and initiation

Liu

Han

Zhang

et al. 2012

Journal of Proteomics

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“…Our observation that one peroxidase unigene is significantly expressed in the upper region of outer stem tissues supports this idea, since bast fiber cell walls are not lignified at this stage. Interestingly, class III peroxidases have been shown to be involved in cell wall loosening and the massive cell expansion associated with cotton fiber elongation (Qin et al, 2008).…”

Section: Hypolignification In Flax Stems Involves Differential Expresmentioning

confidence: 99%

Natural Hypolignification Is Associated with Extensive Oligolignol Accumulation in Flax Stems

et al. 2012

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Flax (Linum usitatissimum) stems contain cells showing contrasting cell wall structure: lignified in inner stem xylem tissue and hypolignified in outer stem bast fibers. We hypothesized that stem hypolignification should be associated with extensive phenolic accumulation and used metabolomics and transcriptomics to characterize these two tissues.1 H nuclear magnetic resonance clearly distinguished inner and outer stem tissues and identified different primary and secondary metabolites, including coniferin and p-coumaryl alcohol glucoside. Ultrahigh-performance liquid chromatography-Fourier transform ion cyclotron resonance-mass spectrometry aromatic profiling (lignomics) identified 81 phenolic compounds, of which 65 were identified, to our knowledge, for the first time in flax and 11 for the first time in higher plants. Both aglycone forms and glycosides of monolignols, lignin oligomers, and (neo)lignans were identified in both inner and outer stem tissues, with a preponderance of glycosides in the hypolignified outer stem, indicating the existence of a complex monolignol metabolism. The presence of coniferin-containing secondary metabolites suggested that coniferyl alcohol, in addition to being used in lignin and (neo)lignan formation, was also utilized in a third, partially uncharacterized metabolic pathway. Hypolignification of bast fibers in outer stem tissues was correlated with the low transcript abundance of monolignol biosynthetic genes, laccase genes, and certain peroxidase genes, suggesting that flax hypolignification is transcriptionally regulated. Transcripts of the key lignan genes Pinoresinol-Lariciresinol Reductase and Phenylcoumaran Benzylic Ether Reductase were also highly abundant in flax inner stem tissues. Expression profiling allowed the identification of NAC (NAM, ATAF1/2, CUC2) and MYB transcription factors that are likely involved in regulating both monolignol production and polymerization as well as (neo)lignan production.

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The ascorbate peroxidase regulated by H2O2 and ethylene is involved in cotton fiber cell elongation by modulating ROS homeostasis

Cited by 60 publications

References 24 publications

GbPDF1 Is Involved in Cotton Fiber Initiation via the Core cis-Element HDZIP2ATATHB2

GbPDF1 Is Involved in Cotton Fiber Initiation via the Core cis-Element HDZIP2ATATHB2

Comparative proteomic analysis reveals the mechanisms governing cotton fiber differentiation and initiation

Natural Hypolignification Is Associated with Extensive Oligolignol Accumulation in Flax Stems

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