The association between circulating APRIL levels and severity of obstructive sleep apnea in Chinese adults

Wen, Wanwan; Sun, Haili; Yang, Yunxiao; Jia, Yixin; Huang, Mengling; Du, Yunhui; Qin, Yanwen; Fang, Fang; Zhang, Ming; Wei, Yongxiang

doi:10.1016/j.cca.2020.05.028

Cited by 4 publications

(4 citation statements)

References 49 publications

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“…Interestingly, a large body of literature reports that upregulated expression of the lncRNAs in hub ceRNA networks causes the same event, i.e., upregulation of the NF-κB, through distinct molecular mechanisms [ 55 , 56 , 57 , 58 ]. As is well known, CIH in OSA patients can cause oxidative stress, systemic inflammation, and NF-κB-dependent inflammatory pathway activation [ 33 , 76 ], which is the potential pathogenesis of complications such as diabetes mellitus and cardiovascular disorders [ 77 , 78 ]. Based on these findings, we, therefore, speculated that the hub ceRNA networks may participate in the occurrence and development of OSA by regulating the activity of the NF-κB pathway and the inflammatory response.…”

Section: Discussionmentioning

confidence: 99%

Integrative Analysis and Experimental Validation of Competing Endogenous RNAs in Obstructive Sleep Apnea

Zhu

Liu

et al. 2023

Biomolecules

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Background: Obstructive sleep apnea (OSA) is highly prevalent yet underdiagnosed. This study aimed to develop a predictive signature, as well as investigate competing endogenous RNAs (ceRNAs) and their potential functions in OSA. Methods: The GSE135917, GSE38792, and GSE75097 datasets were collected from the National Center for Biotechnology Information (NCBI) Gene Expression Omnibus (GEO) database. Weighted gene correlation network analysis (WGCNA) and differential expression analysis were used to identify OSA-specific mRNAs. Machine learning methods were applied to establish a prediction signature for OSA. Furthermore, several online tools were used to establish the lncRNA-mediated ceRNAs in OSA. The hub ceRNAs were screened using the cytoHubba and validated by real-time quantitative reverse transcription-polymerase chain reaction (qRT-PCR). Correlations between ceRNAs and the immune microenvironment of OSA were also investigated. Results: Two gene co-expression modules closely related to OSA and 30 OSA-specific mRNAs were obtained. They were significantly enriched in the antigen presentation and lipoprotein metabolic process categories. A signature that consisted of five mRNAs was established, which showed a good diagnostic performance in both independent datasets. A total of twelve lncRNA-mediated ceRNA regulatory pathways in OSA were proposed and validated, including three mRNAs, five miRNAs, and three lncRNAs. Of note, we found that upregulation of lncRNAs in ceRNAs could lead to activation of the nuclear factor kappa B (NF-κB) pathway. In addition, mRNAs in the ceRNAs were closely correlated to the increased infiltration level of effector memory of CD4 T cells and CD56bright natural killer cells in OSA. Conclusions: In conclusion, our research opens new possibilities for diagnosis of OSA. The newly discovered lncRNA-mediated ceRNA networks and their links to inflammation and immunity may provide potential research spots for future studies.

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Section: Discussionmentioning

confidence: 99%

Integrative Analysis and Experimental Validation of Competing Endogenous RNAs in Obstructive Sleep Apnea

Zhu

Liu

et al. 2023

Biomolecules

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“…The Magnetic Luminex® assay is a magnetic bead-based antibody microarray founded upon the sandwich immunoassay principle, which is used to assess the levels of biomarkers in a single sample [ 31 , 32 ]. We used a panel of unique, custom-made magnetic bead cytokines, including ADAMTS13, to screen biomarkers capable of predicting OSA (R&D Systems, Inc., Minneapolis, MN, USA) [ 33 ]. The assay was performed by a trained investigator who was blinded to the clinical status of the subjects.…”

Section: Methodsmentioning

confidence: 99%

Circulating ADAMTS13 Levels Are Associated with an Increased Occurrence of Obstructive Sleep Apnea

Huang

Liu

et al. 2022

Disease Markers

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Background and Aims. Obstructive sleep apnea (OSA) is strongly associated with obesity, metabolic diseases, coronary artery disease (CAD), stroke, hypertension, and other disorders. This study assessed the relationship between circulating a disintegrin and metalloprotease with a thrombospondin type 1 motif, member 13 (ADAMTS13) levels and the presence of OSA. Materials and Methods. This cross-sectional study included a total of 223 patients. We used a powerful high-throughput multiplexed immunobead-based assay to detect circulating levels of ADAMTS13. The associations between circulating ADAMTS13 levels and OSA were evaluated by multivariate logistic regression analysis. Results. Circulating ADAMTS13 levels were significantly elevated in patients with OSA compared with controls (0.8 vs. 2.7 μg/mL, respectively, P < 0.001 ). After adjusting for confounding factors, circulating ADAMTS13 levels were significantly independently associated with the presence of OSA ( odds ratio = 9.96 , 95% confidence interval (CI) =4.11–24.13, P < 0.001 ). Furthermore, circulating ADAMTS13 levels showed discriminatory accuracy in assessing the presence of OSA (area under the curve: 0.87, 95% CI 0.81–0.93, P < 0.001 ). Conclusion. Circulating ADAMTS13 levels were significantly correlated with the presence of OSA. ADAMTS13 may therefore function as a novel biomarker for monitoring the development and progression of OSA.

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“…Drinkers were participants who consumed alcohol more than three days a week. Serum lipid, high-sensitivity C-reactive protein (hs-CRP), and other indicators were measured as previously described [ 22 , 23 ].…”

Section: Methodsmentioning

confidence: 99%

“…We quickly obtained fasting blood samples after the PSG examination. Procedures for plasma collection, processing, and storage have been previously described [ 22 , 23 ].…”

Section: Methodsmentioning

confidence: 99%

Usefulness of Cathepsin S to Predict Risk for Obstructive Sleep Apnea among Patients with Type 2 Diabetes

et al. 2020

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Background. Obstructive sleep apnea (OSA) was highly prevalent in patients with type 2 diabetes (T2D). Cathepsin S (CTSS), a cysteine protease, is involved in the inflammatory activity in T2D and hypoxia conditions. The aim of the study was to evaluate whether CTSS could be involved in the inflammatory reaction of OSA in patients with T2D. Methods. We included 158 participants in this study matched for age, gender, and body mass index in 4 groups (control, non-OSA&T2D, OSA&non-T2D, and OSA&T2D). After overnight polysomnography, we collected the clinical data including anthropometrical characteristics, blood pressure, and fasting blood samples in the morning. Plasma CTSS concentration was evaluated using the human Magnetic Luminex Assay. Results. Compared with the control group, both the non-OSA&T2D group and the OSA&non-T2D group showed higher CTSS levels. Plasma CTSS expression was significantly increased in subjects with OSA&T2D compared to subjects with non-OSA&T2D. The OSA&T2D group had higher CTSS levels than the OSA&non-T2D group, but there were no statistically significant differences. Plasma CTSS levels showed significant correlation with the apnea-hypopnea index (AHI) (r=0.559, P<0.001) and plasma fasting blood glucose (r=0.427, P<0.001). After adjusting confounding factors, plasma CTSS levels were independently associated with the AHI (Beta: 0.386, 95% confidence intervals (CI): 21.988 to 57.781; P<0.001). Furthermore, we confirmed the higher pinpoint accuracy of plasma CTSS in the diagnosis of OSA (area under the curve: 0.868). Conclusions. Plasma CTSS expression was significantly elevated in the OSA&T2D group and was independently associated with the AHI; it could be a biomarker with a positive diagnostic value on diagnosing OSA among patients with T2D.

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The association between circulating APRIL levels and severity of obstructive sleep apnea in Chinese adults

Cited by 4 publications

References 49 publications

Integrative Analysis and Experimental Validation of Competing Endogenous RNAs in Obstructive Sleep Apnea

Integrative Analysis and Experimental Validation of Competing Endogenous RNAs in Obstructive Sleep Apnea

Circulating ADAMTS13 Levels Are Associated with an Increased Occurrence of Obstructive Sleep Apnea

Usefulness of Cathepsin S to Predict Risk for Obstructive Sleep Apnea among Patients with Type 2 Diabetes

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