The association between extended spectrum beta-lactamase (ESBL) and ampicillin C (AmpC) beta-lactamase genes with multidrug resistance in Escherichia coli isolates recovered from turkeys in Brazil

Abstract

1. The aim of this study was to analyse the association between Escherichia coli isolates recovered from turkeys and the expression of beta-lactamase genes, such as extended spectrum beta-lactamase (ESBL) and ampicillin class C (AmpC). The phenotype of the resistance profile was examined using the association between amoxicillin and ceftiofur resistance. 2. Results showed that 84% from the turkey isolates harboured 4 or 5 genes associated with the CoIV plasmid. In an antibiogram test, 82% of the isolates were multidrug-resistant, the highest levels of resistance being against erythromycin (99%) and amoxicillin (76.1%). ESBL-positive groups were 31% positive for the ctx-m-2 gene, 6.8% were positive for ctx-m-8 and 70% harboured the tem wild gene. 3. All positive isolates from the AmpC beta-lactamase-positive group harboured the cmy-2 gene. The presence of the cmy-2 gene was associated with both the CTX-group genes and resistance to ceftiofur. 4. There was a high prevalence of avian pathogenic E. coli in suspected cases of colibacillosis in turkeys and a high antimicrobial resistance index. The results highlighted the risk of ceftiofur resistance and the presence of both ESBL and AmpC beta-lactamase E. coli in the turkey production chain.

Antibiotic resistance and extended-spectrum β-lactamase in Escherichia coli isolates from imported 1-day-old chicks, ducklings, and turkey poults

“…Furthermore, the β-lactamase-encoding genes bla CTX-M1 and bla CTXM-2 were detected in E. coli isolates from parent stock chickens of broiler Breed A in Dutch farms and in production farms and environmental samples of hatchery units at the broiler hatchery [ 22 ]. ESBL – as well as ampC – producing bacteria are commonly found in the gastrointestinal tract of animals [ 39 ] and have been isolated from turkey [ 40 , 41 ] and poultry [ 42 ]. The gastrointestinal tract of animals is seen as an important reservoir for bacteria that produce β-lactamases and as a potential source for human pathogens to take up these resistance genes [ 39 , 41 , 43 ].…”

“…ESBL – as well as ampC – producing bacteria are commonly found in the gastrointestinal tract of animals [ 39 ] and have been isolated from turkey [ 40 , 41 ] and poultry [ 42 ]. The gastrointestinal tract of animals is seen as an important reservoir for bacteria that produce β-lactamases and as a potential source for human pathogens to take up these resistance genes [ 39 , 41 , 43 ]. ESBL and ampC genes that are located on plasmids are able to spread very rapidly [ 44 ].…”

Molecular Analysis of Escherichia coli Strains Causing Respiratory Troubles in Chickens in Egypt

“…In the present study, ESBL antibiotic resistance genes (blaCTX-M, blaTEM and blaOXA) were selected based on their resistance against cefotaxime, amoxicillin-clavulanic acid and oxacillin respectively. Widespread β-lactam antibiotic prescription in the field of veterinary medicine is one of the factors causing the presence of ESBL antibiotic-resistant bacteria to reach epidemic proportions in recent years [60,61].…”

Antimicrobial Resistance in Farm Animals in Brazil: An Update Overview

“…Reports include animals on farms [36,[136][137][138][141][142][143][144][145][146][147], whole carcasses, or meat pieces available in Brazilian retail markets [69,136,138,140,145,148,149], and exported chicken meat [150][151][152]. The same genes were detected in turkeys with clinical signs of colibacillosis [153]. Although a study stated that the prevalence of ESBL and pAmpC genes was higher in conventional than in free-range chicken, bla CTX-M-2 , bla CTX-M-8 , bla CTX-M-15, and bla CMY-2 were also detected in carcasses of antibiotic-free grown animals [69,140].…”