The association between MICA/MICB polymorphism and respiratory syncytial virus infection in children

Abstract

MICA/MICB gene polymorphisms are related to several cancers and infectious diseases, but there are no reports on the association between MICA/MICB gene polymorphisms and respiratory syncytial virus (RSV) infection. To clarify the association between MICA/MICB gene polymorphisms and infection of RSV in children, we collected fresh blood samples from paediatric patients with and without pneumonia after RSV infection. The MICA/MICB alleles were characterized by PCR sequence-specific primers (PCR-SSP) and PCR sequence-based genotyping (PCR-SBT), and then, the frequency of the MICA/MICB alleles and haplotypes was calculated. The results showed that the frequencies of MICA*002:01 and MICA-A9 in RSV-infected patients were significantly lower than in controls (9% vs. 20%, pc = 0.04). The allele frequency of MICA*002:01 in pneumonia patients (8%) and nonpneumonia patients (9%) was significantly lower than in controls (20%, pc = 0.02). MICA*002:01-MICB*008(Δrel = 0.616), MICA*009-MICB*016 (Δrel = 0.506), and MICA*045-MICB*014 (Δrel = 0.700) showed linkage disequilibrium in patients infected with RSV. The haplotype frequency of MICA*002:01-MICB*005:02 in RSV-infected patients was significantly lower than in controls (10% vs. 16%, pc = 0.033). In conclusion, allele MICA*002:01/A9 and haplotype MICA*002:01-MICB*005:02 were negatively associated with RSV respiratory tract infections.

NK cell‐mediated anti‐leukemia cytotoxicity is enhanced using a NKG2D ligand MICA and anti‐CD20 scfv chimeric protein

“…This system can be utilized to study the function of various known ligands. MICA, a ligand of NKG2D, is a stress induced protein expressed in epithelium, fibroblasts, keratinocytes, endothelial cells, and monocytes and involved in innate and adaptive immune response such as autoimmune diseases , infection , transplant rejection . There are two dozen MICA and MICB variants distributing in different populations with unclear differences in functions.…”

Disease severity in respiratory syncytial virus infection: Role of host genetic variation

“…118 Furthermore, allele MICA*002:01/A9 and haplotype MICA*002:01-MICB*005:02 were negatively associated with RSV respiratory tract infections in another study. 119 (potassium voltage-gated channel subfamily D member 3). 121 Recently, another GWAS study showed that NKG2D gene variation (rs10772271) may predispose infants to bronchiolitis.…”

“…By evaluating polymorphisms within ICAM‐1 , VCAM‐1 , and E‐selectin genes, Krueger et al found no evidence for association of any polymorphism with severe RSV infections . Furthermore, allele MICA *002:01/A9 and haplotype MICA *002:01‐MICB*005:02 were negatively associated with RSV respiratory tract infections in another study . High et al found that human MARCO (an innate immunity scavenger receptor) promoter SNP (rs1318645) that caused loss of gene expression, increased in vitro cellular response to RSV infection, and associated with increased risk of disease severity in children infected with RSV .…”

Prognostic Value of Serum MICA Levels as a Marker of Severity in COVID-19 Patients

“…MICA/B were the rst identi ed ligands for NKG2D whose genes have been mapped to the MHC class I region, in the proximity of HLA-B locus [13]. These highly glycosylated proteins are present in membranebound and soluble isoforms and bear complete polymorphism with more than 100 alleles [13,16,17]. [18].…”