The association between micronucleus, nucleoplasmic bridges, and nuclear buds frequency and the degree of uterine cervical lesions

Gashi, Goneta; Mahovlić, Vesna; Manxhuka-Kërliu, Suzana; Podrimaj-Bytyqi, Arjeta; Gashi, Luljeta; Elezaj, Isa R.

doi:10.1080/1354750x.2018.1428828

Cited by 22 publications

(13 citation statements)

References 60 publications

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“…Such anomalies include nucleoplasmic bridges (NPB) and nuclear buds (NBUD) and are biomarkers of genotoxic events and manifestations of chromosomal instability that often indicate cancer risk. Genetic damage events such as MN, NPB and NBUD provide valid measures of misrepaired DNA breaks [145]. MN, NPB and NBUD formation could be due to multiple molecular mechanisms.…”

Section: Potential Biomarkers Of Chromosomal Abnormalitiesmentioning

confidence: 99%

DNA Damage/Repair Management in Cancers

Alhmoud

Woolley

Moustafa

et al. 2020

Cancers

233

132

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DNA damage is well recognized as a critical factor in cancer development and progression. DNA lesions create an abnormal nucleotide or nucleotide fragment, causing a break in one or both chains of the DNA strand. When DNA damage occurs, the possibility of generated mutations increases. Genomic instability is one of the most important factors that lead to cancer development. DNA repair pathways perform the essential role of correcting the DNA lesions that occur from DNA damaging agents or carcinogens, thus maintaining genomic stability. Inefficient DNA repair is a critical driving force behind cancer establishment, progression and evolution. A thorough understanding of DNA repair mechanisms in cancer will allow for better therapeutic intervention. In this review we will discuss the relationship between DNA damage/repair mechanisms and cancer, and how we can target these pathways.

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Section: Potential Biomarkers Of Chromosomal Abnormalitiesmentioning

confidence: 99%

DNA Damage/Repair Management in Cancers

Alhmoud

Woolley

Moustafa

et al. 2020

Cancers

233

132

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“…The association between the frequency of MN, NPB, and NBUD and the development of benign and malignant tumors has been reported in other studies. The report shows an increase in these biomarkers' frequency in patients with various types of lesions and healthy relatives of cancer patients Gashi et al, 2018). MN, NPB, and NBUD are nuclear anomalies commonly seen in cancer and represent general phenotypes of chromosomal instability.…”

Section: Evaluation Of Npb and Nbudmentioning

confidence: 85%

Detection of Micronucleus, Nucleoplasmic Bridges, and Nuclear Buds Frequency in Peripheral Blood Lymphocytes of Cancer Patient Post-Radiation Fractionated

Kisnanto

Lusiyanti

Erawati

et al. 2021

Nusantara Science and Technology Proceedings

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Simple measurement of cytogenetic damage would be of great value for studying genetic risk factors, especially in radiotherapy for cancer patients. One cytogenetic technique that is easy and simple to quantify the damage caused by radiation exposure in cultured human lymphocytes is the micronucleus (MN). This research was conducted to study the induction of micronucleus (MN), Nucleoplasmic Bridge (NPB), and Nuclear Buds (NBUDs) in cancer patients after administration of fractionated radiation exposure total of 20 Gy. Peripheral blood lymphocyte samples obtained from eleven cancer patients as the study group and eleven from the healthy people as the control group were assessed. Both samples were then cultured and added cytochalasin-B to arrest cells during the cytokinesis stage. Its characteristics were observed in binucleated cells (BNC) with cytochalasin blocked micronuclei (CBMN) assay procedure. The number of MN, NPB, and NBUDs was evaluated per 1000 BNC for both the study group and control. The results showed that there was a statistically signif-icant difference (P <0.05) between the frequency of MN in the study group (82.18±39.93) compared to controls (13.18±4.94). Besides, the number of NPB and NBUD in the study group is relatively low. In conclusion, the iden-tification of MN formation in peripheral blood lymphocytes of post-radiation cancer patients has other molecular mechanisms such as NPB and NBUD. Also, demographic factors such as age can influence the appearance of MN, NPB, and NBUDs.

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“…The MN frequency in BEC was found to have a statistically significant positive correlation with UEC and PBL in the patient group (p < 0.006, p < 0.015, respectively; Table 3). While there is a lack of literature studying the MN frequency in BEC among bladder cancer patients, a number of papers have explored the MN frequency in BEC among patients with head and neck cancer 9 , oral cancer 38 , breast cancer, and uterine cancer 39 , as well as among those with cervical pre-cancer and cancer 40,41 . These studies are consistent with our findings, showing that the MN index in PBL is significantly reflected by the MN assay in oral exfoliated epithelial cells.…”

Section: Discussionmentioning

confidence: 99%

The frequencies of micronuclei, nucleoplasmic bridges and nuclear buds as biomarkers of genomic instability in patients with urothelial cell carcinoma

Podrimaj-Bytyqi

Borovečki

Selimi

et al. 2018

Sci Rep

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Bladder urothelial cell carcinoma (UCC) is an increasingly prevalent cancer worldwide, and thus, gaining a better understanding of its identifiable risk factors is a global priority. This study addressed this public health need with the understanding that cancer-initiating events, such as chromosome breakage, loss and rearrangement, can be reasonably used as biomarkers to evaluate an individual’s cancer risk. Overall, forty bladder cancer patients and twenty controls were evaluated for genomic instability. To the best of the investigators’ knowledge, this is the first study to perform micronucleus (MN) assays simultaneously in urothelial exfoliated cells (UEC), buccal exfoliated cells (BEC), and peripheral blood lymphocytes (PBL) in first-diagnosed, non-smoker bladder UCC patients. Additionally, the frequency of nucleoplasmic bridges (NPBs) and nuclear buds (NBUDs) in PBL was evaluated. The MN frequencies in UEC, BEC, and PBL, as well as the frequencies of NPBs and NBUDs, were significantly higher in patients than in controls. In conclusion, MN assays, particularly in UEC, may be used to identify individuals who are at high risk of developing UCC, as single or as additional triage test to UroVysion FISH test. Our results further validate the efficacy of biomarkers, such as MN, NPBs, and NBUDs, as predictors of genomic instability.

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The association between micronucleus, nucleoplasmic bridges, and nuclear buds frequency and the degree of uterine cervical lesions

Abstract: Although larger studies are needed, our data support the predictive value of MN, NPB and NBUD as biomarkers of genomic instability for evaluation of risk level of cancer diseases.

Cited by 22 publications

References 60 publications

DNA Damage/Repair Management in Cancers

DNA Damage/Repair Management in Cancers

Detection of Micronucleus, Nucleoplasmic Bridges, and Nuclear Buds Frequency in Peripheral Blood Lymphocytes of Cancer Patient Post-Radiation Fractionated

The frequencies of micronuclei, nucleoplasmic bridges and nuclear buds as biomarkers of genomic instability in patients with urothelial cell carcinoma

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