The ATPase subunit 6 gene of Leptomonas seymouri (Trypanosomatidae) is transcribed and edited as a polycistronic mRNA

Bessolitsina, E. A.; Fedyakov, A. V.; Merzlyak, Ekaterina M.; Kolesnikov, Alexander

doi:10.1007/s11008-005-0007-y

Cited by 4 publications

(2 citation statements)

References 20 publications

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“…Partially edited cDNA products were obtained with two pre-edited primer sets (see Supplemental Fig. S3) that were located on adjacent maxicircle genes (ND7-CO3, CO3-Cyb, and CO2-Murf2), indicating the presence of unprocessed maxicircle transcripts in the TAP pull-down, as was previously reported (Koslowsky and Yahampath 1997;Bessolitsyna et al 2005). The presence of partially edited maxicircle transcripts was also examined by RT-PCR using downstream edited and upstream unedited primer sets for the pan-edited ND8 transcripts and for the Cyb-A6 unprocessed transcripts (Fig.…”

Section: Discovery Of the L*-complexesmentioning

confidence: 56%

Uridine insertion/deletion RNA editing in trypanosomatid mitochondria: In search of the editosome

Osato

Rogers²,

Guo³

et al. 2009

RNA

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The RNA ligase-containing or L-complex is the core complex involved in uridine insertion/deletion RNA editing in trypanosome mitochondria. Blue native gels of glycerol gradient-separated fractions of mitochondrial lysate from cells transfected with the TAP-tagged editing protein, LC-8 (TbMP44/KREPB5), show a ;1 MDa L-complex band and, in addition, two minor higher molecular weight REL1-containing complexes: one (L*a) co-sedimenting with the L-complex and running in the gel at around 1.2 MDa; the other (L*b) showing a continuous increase in molecular weight from 1 MDa to particles sedimenting over 70S. The L*b-complexes appear to be mainly composed of L-complex components, since polypeptide profiles of L-and L*b-complex gradient fractions were similar in composition and L*b-complex bands often degraded to L-complex bands after manipulation or freeze-thaw cycles. The L*a-complex may be artifactual since this gel shift can be produced by various experimental manipulations. However, the nature of the change and any cellular role remain to be determined. The L*b-complexes from both lysate and TAP pull-down were sensitive to RNase A digestion, suggesting that RNA is involved with the stability of the L*b-complexes. The MRP1/2 RNA binding complex is localized mainly in the L*b-complexes in substoichiometric amounts and this association is RNase sensitive. We suggest that the L*b-complexes may provide a scaffold for dynamic interaction with other editing factors during the editing process to form the active holoenzyme or ''editosome.''

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Section: Discovery Of the L*-complexesmentioning

confidence: 56%

Uridine insertion/deletion RNA editing in trypanosomatid mitochondria: In search of the editosome

Osato

Rogers²,

Guo³

et al. 2009

RNA

View full text Add to dashboard Cite

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“…Gerasimov et al (2018); 4. Bessolitsyna, Fediakov, Merzliak, & Kolesnikov (2005); Kolesnikov, Merzliak, Bessolitsyna, Fediakov, & Shoenian (2003); Merzlyak et al (2001); 5. Kolesnikov et al (2003); 6.…”

Section: Figurementioning

confidence: 99%

High throughput sequencing revolution reveals conserved fundamentals of U‐indel editing

2018

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Among Euglenozoans, mitochondrial RNA editing occurs in the diplonemids and in the kinetoplastids that include parasitic trypanosomes. Yet U-indel editing, in which open reading frames (ORFs) on mRNAs are generated by insertion and deletion of uridylates in locations dictated by guide RNAs, appears confined to kinetoplastids. The nature of guide RNA and edited mRNA populations has been cursorily explored in a surprisingly extensive number of species over the years, although complete sets of fully edited mRNAs for most kinetoplast genomes are largely missing. Now, however, high throughput sequencing technologies have had an enormous impact on what we know and will learn about the mechanisms, benefits, and final edited products of U-indel editing. Tools including PARERS, TREAT, and T-Aligner function to organize and make sense of U-indel mRNA transcriptomes, which are comprised of mRNAs harboring uridylate indels both consistent and inconsistent with translatable products. From high throughput sequencing data come arguments that partially edited mRNAs containing "junction regions" of noncanonical editing are editing intermediates, and conversely, arguments that they are dead-end products. These data have also revealed that the percent of a given transcript population that is fully or partially edited varies dramatically between transcripts and organisms. Outstanding questions that are being addressed include the prevalence of sequences that apparently encode alternative ORFs, diversity of editing events in ORF termini and 5' and 3' untranslated regions, and the differences that exist in this byzantine process between species. High throughput sequencing technologies will also undoubtedly be harnessed to probe U-indel editing's evolutionary origins. This article is categorized under: RNA Processing > RNA Editing and Modification RNA Evolution and Genomics > Computational Analyses of RNA.

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Unexplained complexity of the mitochondrial genome and transcriptome in kinetoplastid flagellates

2005

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Kinetoplastids are flagellated protozoans, whose members include the pathogens Trypanosoma brucei, T. cruzi and Leishmania species, that are considered among the earliest diverging eukaryotes with a mitochondrion. This organelle has become famous because of its many unusual properties, which are unique to the order Kinetoplastida, including an extensive kinetoplast DNA network and U-insertion/deletion type RNA editing of its mitochondrial transcripts. In the last decade, considerable progress has been made in elucidating the complex machinery of RNA editing. Moreover, our understanding of the structure and replication of kinetoplast DNA has also dramatically improved. Much less however, is known, about the developmental regulation of RNA editing, its integration with other RNA maturation processes, stability of mitochondrial mRNAs, or evolution of the editing process itself. Yet the profusion of genomic data recently made available by sequencing consortia, in combination with methods of reverse genetics, hold promise in understanding the complexity of this exciting organelle, knowledge of which may enable us to fight these often medically important protozoans.

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The ATPase subunit 6 gene of Leptomonas seymouri (Trypanosomatidae) is transcribed and edited as a polycistronic mRNA

Cited by 4 publications

References 20 publications

Uridine insertion/deletion RNA editing in trypanosomatid mitochondria: In search of the editosome

Uridine insertion/deletion RNA editing in trypanosomatid mitochondria: In search of the editosome

High throughput sequencing revolution reveals conserved fundamentals of U‐indel editing

Unexplained complexity of the mitochondrial genome and transcriptome in kinetoplastid flagellates

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