The bacterial storage compound poly‐β‐hydroxybutyrate protects <i>Artemia franciscana</i> from pathogenic <i>Vibrio campbellii</i>

Defoirdt, Tom; Halet, Dirk; Vervaeren, Han; Boon, Nico; Wiele, Tom Van de; Sorgeloos, Patrick; Bossier, Peter; Verstraete, W.

doi:10.1111/j.1462-2920.2006.01161.x

Cited by 152 publications

(147 citation statements)

References 45 publications

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“…Lee et al [27] evaluated cell adhesion of various aliphatic polyesters and they found that (RS)-PHB have low cell adhesion because of the surface accumulation of methyl groups. Defoirdt et al [28] reported that short-chain fatty acids like PHB showed a strong growth-inhibitory against pathogenic V. campbellii . Recently PHB was proven to be an immunostimulant in fish [29].…”

Section: Discussionmentioning

confidence: 99%

Antiadhesive activity of poly-hydroxy butyrate biopolymer from a marine Brevibacterium casei MSI04 against shrimp pathogenic vibrios

et al. 2014

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BackgroundVibrio pathogens are causative agents of mid-culture outbreaks, and early mortality syndrome and secondary aetiology of most dreadful viral outbreaks in shrimp aquaculture. Among the pathogenic vibrios group, Vibrio alginolyticus and V. harveyi are considered as the most significant ones in the grow-out ponds of giant black tiger shrimp Penaeus monodon in India. Use of antibiotics was banned in many countries due to the emergence of antibiotic-resistant strains and accumulation of residual antibiotics in harvested shrimp. There is an urgent need to consider the use of alternative antibiotics for the control of vibriosis in shrimp aquaculture. Biofilm formation is a pathogenic and/or establishment mechanism of Vibrio spp. This study aims to develop novel safe antibiofilm and/or antiadhesive process using PHB to contain vibrios outbreaks in shrimp aquaculture.ResultsIn this study a poly-hydroxy butyrate (PHB) polymer producing bacterium Brevibacterium casei MSI04 was isolated from a marine sponge Dendrilla nigra and production of PHB was optimized under submerged-fermentation (SmF) conditions. The effect of carbon, nitrogen and mineral sources on PHB production and enhanced production of PHB by response surface methods were demonstrated. The maximum PHB accumulation obtained was 6.74 g/L in the optimized media containing 25 g/L starch as carbon source, 96 h of incubation, 35°C and 3% NaCl. The highest antiadhesive activity upto 96% was recorded against V. vulnificus, and V. fischeri, followed by 92% against V. parahaemolyticus and V. alginolyticus and 88% inhibition was recorded against V. harveyi.ConclusionIn this study, a thermostable biopolymer was chemically characterized as PHB based on 1HNMR spectra, FT-IR and GC-MS spectra. The NMR spectra revealed that the polymer was an isocratic homopolymer and it also confirmed that the compound was PHB. The antiadhesive activity of PHB was determined in microtitre plate assay and an effective concentration (EC) of PHB (200 μl containing 0.6 mg PHB) was confirmed by confocal laser scanning microscopic analysis of vibrio biofilm on pre-treated glass and polystyrene surfaces. This is a first report on anti-adhesive activity of PHB against prominent vibrio pathogens in shrimp aquaculture.Electronic supplementary materialThe online version of this article (doi:10.1186/s12934-014-0114-3) contains supplementary material, which is available to authorized users.

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Section: Discussionmentioning

confidence: 99%

Antiadhesive activity of poly-hydroxy butyrate biopolymer from a marine Brevibacterium casei MSI04 against shrimp pathogenic vibrios

et al. 2014

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“…[37], [38], [39], [40], [41], [42]. Taken together, this suggests that a reduction of M. rhodesianum abundance allows colonization or over-growth by other bacteria, some of which are pathogenic.…”

Section: Discussionmentioning

confidence: 99%

Inter Individual Variations of the Fish Skin Microbiota: Host Genetics Basis of Mutualism?

Boutin

Sauvage²,

Bernatchez³

et al. 2014

PLoS ONE

131

119

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The commensal microbiota of fish skin is suspected to provide a protection against opportunist infections. The skin of fish harbors a complex and diverse microbiota that closely interacts with the surrounding water microbial communities. Up to now there is no clear evidence as to whether the host regulates the recruitment of environmental bacteria to build a specific skin microbiota. To address this question, we detected Quantitative Trait Loci (QTL) associated with the abundance of specific skin microbiota bacterial strains in brook charr (Salvelinus fontinalis), combining 16S RNA tagged-amplicon 454 pyrosequencing with genetic linkage analysis. Skin microbiota analysis revealed high inter-individual variation among 86 F2 fish progeny based upon the relative abundance of bacterial operational taxonomic units (OTUs). Out of those OTUs, the pathogenic strain Flavobacterium psychrophilum and the non-pathogenic strain Methylobacterium rhodesianum explained the majority of inter-individual distances. Furthermore, a strong negative correlation was found between Flavobacterium and Methylobacterium, suggesting a mutually competitive relationship. Finally, after considering a total of 266 markers, genetic linkage analysis highlighted three major QTL associated with the abundance of Lysobacter, Rheinheimera and Methylobacterium. All these three genera are known for their beneficial antibacterial activity. Overall, our results provide evidence that host genotype may regulate the abundance of specific genera among their surface microbiota. They also indicate that Lysobacter, Rheinheimera and Methylobacterium are potentially important genera in providing protection against pathogens.

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“…PHB dapat mengaktifkan mekanisme pertahanan nonspesifik dengan cara melepaskan asam lemak yang dapat melindungi udang dari patogen. Asam lemak dapat menyediakan energi bagi udang dan menghambat pertumbuhan patogen (Defoirdt et al, 2007). Penggunaan PHB pada Artemia dapat meningkatkan sistem imun dengan menghambat pertumbuhan bakteri patogen (Nhan et al, 2010).…”

Section: Pendahuluanunclassified

Growth and immune response of Litopenaeus vannamei fed on β-(1, 3) glucan and poly-β hydroxybutyrate

Sarmin

Suprayudi

Jusadi

et al. 2015

Jurnal Akuakultur Indonesia

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This research was aimed to examine the growth performance and non-specific immune response of Pacific white shrimp (Litopenaeus vannamei) fed on the diet supplemented with β-(1,3) glucan (BG) and poly-β-hydroxybutyrate (PHB) as feed additives. Shrimp juvenile at an initial body weight of 2.06±0.03 g was randomly distributed into 12 units of aquaria at a density of 20 shrimps/tank and reared for 42 days. The treatments applied in this study were control (without feed additives), 1.5 g/kg BG, 10 g/kg PHB and 1,5 g/kg BG+10 g/kg PHB. Results showed that shrimp fed on 1.5 /kg BG-supplemented feed had significantly higher growth performance and non-specific immune response.

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The bacterial storage compound poly‐β‐hydroxybutyrate protects Artemia franciscana from pathogenic Vibrio campbellii

Cited by 152 publications

References 45 publications

Antiadhesive activity of poly-hydroxy butyrate biopolymer from a marine Brevibacterium casei MSI04 against shrimp pathogenic vibrios

Antiadhesive activity of poly-hydroxy butyrate biopolymer from a marine Brevibacterium casei MSI04 against shrimp pathogenic vibrios

Inter Individual Variations of the Fish Skin Microbiota: Host Genetics Basis of Mutualism?

Growth and immune response of Litopenaeus vannamei fed on β-(1, 3) glucan and poly-β hydroxybutyrate

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