The barley MATE gene, HvAACT1, increases citrate efflux and Al3+ tolerance when expressed in wheat and barley

Zhou, Gaofeng; Delhaize, Emmanuel; Zhou, Meixue; Ryan, Peter R.

doi:10.1093/aob/mct135

Cited by 105 publications

(84 citation statements)

References 58 publications

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“…It will be interesting to determine whether these differences in regulation mechanisms correlate with differences in environmental conditions. Al 3+ tolerance was recently investigated in three transgenic barley lines, which express AtFRD3, HvAACT1, or SbMATE under the regulation of a constitutive maize ubiquitin promoter (Zhou et al 2013;Zhou et al 2014). All three transgenic barley plants showed a more Al 3+ tolerant phenotype than control plants, with the levels of Al 3+ tolerance of the three transgenic lines being almost identical (Zhou et al 2014).…”

Section: + Detoxificationmentioning

confidence: 99%

The multidrug and toxic compound extrusion (MATE) family in plants

Takanashi

Shitan

Yazaki

2014

Plant Biotechnology

128

113

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Multidrug and toxic compound extrusion (MATE) transporters are a family of cation antiporters occurring in most organisms from prokaryotes to eukaryotes. This family constitutes one of the largest transporter families in plants, with, for example, more than 50 MATE genes in the Arabidopsis genome. Moreover, MATE transporters are involved in a wide variety of physiological functions throughout plant development, transporting a broad range of substrates such as organic acids, plant hormones and secondary metabolites. This review categorizes plant MATE transporters according to their physiological roles and summarizes their tissue specificity, membrane localization, and transport substrates. We also review the molecular evolutionary development of plant MATE transporters.

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Section: + Detoxificationmentioning

confidence: 99%

The multidrug and toxic compound extrusion (MATE) family in plants

Takanashi

Shitan

Yazaki

2014

Plant Biotechnology

128

113

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“…In wheat, although some studies have used soil experiments in glasshouse or growth chambers (Pereira et al, 2010;Pereira et al, 2015;Tang et al, 2003;Zhou et al, 2013), most of the reports assessing Al stress response have used hydroponic culture. The advantages in using hydroponics are the isolation of the Al stress, greater control of environmental conditions and easy observation of the roots.…”

Section: Discussionmentioning

confidence: 99%

Initial root length in wheat is highly correlated with acid soil tolerance in the field

Pereira

2018

Sci. agric. (Piracicaba, Braz.)

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ABSTRACT:In acid soils, toxic aluminum ions inhibit plant root growth. In order to discriminate aluminum (Al) tolerance, trustful screening techniques are required. In this study, 20 wheat cultivars, showing different levels of Al tolerance, were evaluated in a short-term soil experiment to access their relative root length (RRL). Moreover, the alleles of two important genes (TaALMT1 and TaMATE1B) for Al tolerance in wheat were discriminated. Both of these genes encode membrane transporters responsible for the efflux of organic acids by the root apices that are thought to confer tolerance by chelating Al. Genotypes showing TaALMT1 alleles V and VI and an insertion at the TaMATE1B promoter were among the ones showing greater RRL. Mechanisms of Al tolerance, which are not associated with organic acid efflux, can be potentially present in two cultivars showing greater RRL among the ones carrying inferior TaALMT1 and TaMATE1B alleles.The RRL data were highly correlated with wheat performance in acid soil at three developmental stages, tillering (r = -0.93, p < 0.001), silking (r = -0.91, p < 0.001) and maturation (r = -0.90, p < 0.001), as well as with the classification index of aluminum toxicity in the field (r = -0.92, p < 0.001). Since the RRL was obtained after only six days of growth and it is highly correlated with plant performance in acid soil under field conditions, the short-term experiment detailed here is an efficient and rapid method for reliable screening of wheat Al tolerance.

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“…Following the example of relative root growth (RRG), the SE , where x and y represent mean growth in the control and treatment conditions and SE x and SE y are the SE values for root growth in control and treatment conditions, respectively. To statistically compare relative data from one line with another, we adapted an approach based on overlapping confidence limits described previously (Zhou et al, 2013).…”

Section: Discussionmentioning

confidence: 99%

“…The solutions were aerated to mix the solution and replaced every 3 d. After 7 d, the longest root length was measured again, and the RRL was calculated as root growth in Al 3+ treatment compared with the root growth in control solution. To statistically compare relative data from one line with another, we adapted an approach based on overlapping confidence limits described previously (Zhou et al, 2013). …”

Section: Al Resistancementioning

confidence: 99%

Altered Expression of a Malate-Permeable Anion Channel, OsALMT4, Disrupts Mineral Nutrition

et al. 2017

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ORCID IDs: 0000-0003-1555-4754 (J.L.); 0000-0003-3009-7854 (M.Z.); 0000-0002-1376-9543 (P.R.R.).Aluminum-activated malate transporters (ALMTs) form a family of anion channels in plants, but little is known about most of its members. This study examined the function of OsALMT4 from rice (Oryza sativa). We show that OsALMT4 is expressed in roots and shoots and that the OsALMT4 protein localizes to the plasma membrane. Transgenic rice lines overexpressing (OX) OsALMT4 released malate from the roots constitutively and had 2-fold higher malate concentrations in the xylem sap than nulls, indicating greater concentrations of malate in the apoplast. OX lines developed brown necrotic spots on the leaves that did not appear on nulls. These symptoms were not associated with altered concentrations of any mineral element in the leaves, although the OX lines had higher concentrations of Mn and B in their grain compared with nulls. While total leaf Mn concentrations were not different between the OX and null lines, Mn concentrations in the apoplast were greater in the OX plants. The OX lines also displayed increased expression of Mn transporters and were more sensitive to Mn toxicity than null plants. We showed that the growth of wild-type rice was unaffected by 100 mM Mn in hydroponics but, when combined with 1 mM malate, this concentration inhibited growth. We conclude that increasing OsALMT4 expression affected malate efflux and compartmentation within the tissues, which increased Mn concentrations in the apoplast of leaves and induced the toxicity symptoms. This study reveals new links between malate transport and mineral nutrition.

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The barley MATE gene, HvAACT1, increases citrate efflux and Al3+ tolerance when expressed in wheat and barley

Abstract: Increased HvAACT1 expression in both cereal species was associated with increased citrate efflux from root apices and enhanced Al(3+) tolerance, thus demonstrating that biotechnology can complement traditional breeding practices to increase the Al(3+) tolerance of important crop plants.

Cited by 105 publications

References 58 publications

The multidrug and toxic compound extrusion (MATE) family in plants

The multidrug and toxic compound extrusion (MATE) family in plants

Initial root length in wheat is highly correlated with acid soil tolerance in the field

Altered Expression of a Malate-Permeable Anion Channel, OsALMT4, Disrupts Mineral Nutrition

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