The barley stem rust-resistance gene
            <i>Rpg1</i>
            is a novel disease-resistance gene with homology to receptor kinases

Brueggeman, Robert; Rostoks, Nils; Kudrna, David; Kilian, Andrzej; Han, Feng; Chen, J.; Druka, Arnis; Steffenson, Brian J.; Kleinhofs, A.

doi:10.1073/pnas.142284999

Cited by 382 publications

(259 citation statements)

References 41 publications

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“…Morex (GenBank accession no. AF509748) was isolated after SrfI and HpaI restriction of plasmid pNRG028, which is a 13.4-kb NotI subclone of bacterial artificial chromosome clone 426c16 containing the complete Rpg1 gene sequence (15). The SrfI͞ HpaI DNA fragment was ligated to HindIII adapters (S1107S and S1140S, New England Biolabs) and cloned into the HindIIIrestricted Agrobacterium vector pJH260 (17) without restoring the HindIII site.…”

Section: Methodsmentioning

confidence: 99%

“…Integrity and quantity of total RNA was validated by formaldehyde denaturing gel electrophoresis. One microgram of total RNA was used for conversion to cDNA with the Reverse Transcription System (Promega) and oligo(dT) 15 primer. Quantification of the Rpg1 and reference gene GAPDH transcripts was performed on the Rotor-Gene 2000 real-time PCR cycler (Corbett Research, Mortlake, New South Wales, Australia) using the QuantiTect SYBR Green PCR system (Qiagen) with genespecific primers.…”

Section: Methodsmentioning

confidence: 99%

“…tritici in barley was cloned by a map-based approach (15). Rpg1 was identified by high-resolution genetic and physical mapping, comparative sequencing of multiple alleles from resistant and susceptible lines, and analysis of a rare recombinant that combined portions of the gene from resistant and susceptible parents.…”

Section: R Esistance Of Flax (Linum Usitatissimum) To the Rust Fungusmentioning

confidence: 99%

“…1). The Rpg1 gene in other investigated susceptible barley cultivars contains either multiple mutations or stop codons, or the gene cannot be amplified with appropriate primers (15).…”

Section: R Esistance Of Flax (Linum Usitatissimum) To the Rust Fungusmentioning

confidence: 99%

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Genetically engineered stem rust resistance in barley using the Rpg1 gene

Horvath

Rostoks

Brueggeman

et al. 2002

Proc. Natl. Acad. Sci. U.S.A.

111

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The stem-rust-susceptible barley cv. Golden Promise was transformed by Agrobacterium-mediated transformation of immature zygotic embryos with the Rpg1 genomic clone of cv. Morex containing a 520-bp 5 promoter region, 4,919-bp gene region, and 547-bp 3 nontranscribed sequence. Representatives of 42 transgenic barley lines obtained were characterized for their seedling infection response to pathotype Pgt-MCC of the stem rust fungus Puccinia graminis f. sp. tritici. Golden Promise was converted from a highly susceptible cultivar into a highly resistant one by transformation with the dominant Rpg1 gene. A single copy of the gene was sufficient to confer resistance against stem rust, and progenies from several transformants segregated in a 3:1 ratio for resistance͞susceptibility as expected for Mendelian inheritance. These results unequivocally demonstrate that the DNA segment isolated by map-based cloning is the functional Rpg1 gene for stem rust, resistance. One of the remarkable aspects about the transformants is that they exhibit a higher level of resistance than the original sources of Rpg1 (cvs. Chevron and Peatland). In most cases, the Golden Promise transformants exhibited a highly resistant reaction where no visible sign of infection was evident. Hypersensitive necrotic ''fleck'' reactions were also observed, but less frequently. With both infection types, pathogen sporulation was prevented. Southern blot and RT-PCR analysis revealed that neither Rpg1 gene copy number nor expression levels could account for the increased resistance observed in Golden Promise transformants. Nevertheless, this research demonstrates that stem-rust-susceptible barley can be made resistant by transformation with the cloned Rpg1 gene.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: R Esistance Of Flax (Linum Usitatissimum) To the Rust Fungusmentioning

confidence: 99%

“…1). The Rpg1 gene in other investigated susceptible barley cultivars contains either multiple mutations or stop codons, or the gene cannot be amplified with appropriate primers (15).…”

Section: R Esistance Of Flax (Linum Usitatissimum) To the Rust Fungusmentioning

confidence: 99%

See 2 more Smart Citations

Genetically engineered stem rust resistance in barley using the Rpg1 gene

Horvath

Rostoks

Brueggeman

et al. 2002

Proc. Natl. Acad. Sci. U.S.A.

111

View full text Add to dashboard Cite

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“…An interesting and valuable rust resistance gene for stem rust Rpg1 has been cloned from barley (Brueggeman et al 2002). This gene, which is not from the most common NBS-LRR class of plant disease resistance genes, has provided durable stem rust resistance in barley.…”

Section: Discovery and Usage Of Genes For Improved Disease Resistancementioning

confidence: 99%

Genetic contributions to agricultural sustainability

Dennis

Ellis

Green

et al. 2007

Phil. Trans. R. Soc. B

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The current tools of enquiry into the structure and operation of the plant genome have provided us with an understanding of plant development and function far beyond the state of knowledge that we had previously. We know about key genetic controls repressing or stimulating the cascades of gene expression that move a plant through stages in its life cycle, facilitating the morphogenesis of vegetative and reproductive tissues and organs. The new technologies are enabling the identification of key gene activity responses to the range of biotic and abiotic challenges experienced by plants. In the past, plant breeders produced new varieties with changes in the phases of development, modifications of plant architecture and improved levels of tolerance and resistance to environmental and biotic challenges by identifying the required phenotypes in a few plants among the large numbers of plants in a breeding population. Now our increased knowledge and powerful gene sequence-based diagnostics provide plant breeders with more precise selection objectives and assays to operate in rationally planned crop improvement programmes. We can expect yield potential to increase and harvested product quality portfolios to better fit an increasing diversity of market requirements. The new genetics will connect agriculture to sectors beyond the food, feed and fibre industries; agri-business will contribute to public health and will provide high-value products to the pharmaceutical industry as well as to industries previously based on petroleum feedstocks and chemical modification processes.

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