1963
DOI: 10.1083/jcb.19.2.279
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THE BASE COMPOSITION OF RIBONUCLEIC ACID IN LAMPBRUSH CHROMOSOMES, NUCLEOLI, NUCLEAR SAP, AND CYTOPLASM OF TRITURUS OOCYTES

Abstract: The base composition of RNA's extracted from chromosomes, nucleoli, nuclear sap, and cytoplasm of Triturus oocytes has been determined by microelectrophorcsis. The chromosomal RNA has a content of guanine+cytosine equal to that of DNA, but there is no complementarity in the composition as for DNA. Nuclear sap contains a highly variable RNA with a tendency towards high uracil values. Nucleolar and cytoplasmic RNA's are similar in composition and both are of the guanine-cytosine rich type. The chromosomes and nu… Show more

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Cited by 87 publications
(14 citation statements)
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“…), che hanno portato ad un notevole approfondimento delle nostre conoscenze sulla morfologia e sulla struttura dei cromosomi lampbrush degli ovociti degli Anfibi; non si puo inoltre dimenticare il contributo dato da altri ricercatori, in particolare Gm'iNoT e DANON (1953), WISCHNITZER (1957,1961), SRIVASTAVA e BHATNAGAR (1962), MACGREGOR e CALLAN (1962), MACGREGOR (1963), MACGREGOR e UzzELL (1964), Mll.LER (1964a). Molto importanti sono anche alcune ricerche condotte con metodiche biochimiche, che hanna notevolmente chiarito il significato funzionale dei bivalenti lampbrush (GALL e CALLAN 1962, lzAwA, .Al.LFREY e MIRsKY 1963, EDSTROM e GALL 1963, DAVIDSON, .Al.LFREY e MIRSKY 1964. Confrontando i risultati di questi studi con quelli gi!t noti per Drosophila e con quelli ottenuti da BEERMANN e Coll.…”
Section: Introduzioneunclassified
“…), che hanno portato ad un notevole approfondimento delle nostre conoscenze sulla morfologia e sulla struttura dei cromosomi lampbrush degli ovociti degli Anfibi; non si puo inoltre dimenticare il contributo dato da altri ricercatori, in particolare Gm'iNoT e DANON (1953), WISCHNITZER (1957,1961), SRIVASTAVA e BHATNAGAR (1962), MACGREGOR e CALLAN (1962), MACGREGOR (1963), MACGREGOR e UzzELL (1964), Mll.LER (1964a). Molto importanti sono anche alcune ricerche condotte con metodiche biochimiche, che hanna notevolmente chiarito il significato funzionale dei bivalenti lampbrush (GALL e CALLAN 1962, lzAwA, .Al.LFREY e MIRsKY 1963, EDSTROM e GALL 1963, DAVIDSON, .Al.LFREY e MIRSKY 1964. Confrontando i risultati di questi studi con quelli gi!t noti per Drosophila e con quelli ottenuti da BEERMANN e Coll.…”
Section: Introduzioneunclassified
“…We know that the total concentration of RNA (Edstrom and Gall, 1963) and DNA (Gall, 1969) in amphibian GVs is trivial relative to protein, but we do not know about possible contributions from small molecules other than ions. Thus, our estimate of protein concentration could be slightly high.…”
Section: Nucleoplasmmentioning
confidence: 99%
“…An even stronger impetus was provided by the later availability of micromanipulators and microscale biochemical methods with which the RNA content and base composition of microdissected cell samples could be measured at the picogram level (82). The RNA base composition was determined with the standard errors of the mean typically within 1-2% of the average (84,151). It was through this method that the existence of mRNA in single eukaryotic cells (initially termed "nonribosomal DNA-like RNA") was first distinguished from rRNA or tRNA.…”
Section: Enhanced Nervous Activitymentioning
confidence: 99%