The basic helix-loop-helix transcription factor, bHLH11 functions in the iron-uptake system in Arabidopsis thaliana

Tanabe, Noriaki; Noshi, Masahiro; Mori, Daisuke; Nozawa, Kotaro; Tamoi, Masahiro; Shigeoka, Shigeru

doi:10.1007/s10265-018-1068-z

Cited by 61 publications

(63 citation statements)

References 59 publications

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“…However, no T-DNA was identified within the bHLH11 gene in the WiscDsLoxHs168_11D; and bHLH11 mRNA was slightly increased in the homozygous line of SAIL_196_A11 with a T-DNA in the promoter region (data not shown). Although the homozygous line of SAIL_196_A11 was lethal according to the recent study (Tanabe et al 2019), we observed that they developed as well as the wild type. Thus, we employed the CRISPR-Cas9 system to edit bHLH11 .…”

Section: Resultssupporting

confidence: 49%

“…Recently, it was reported that the overexpression of bHLH11 leads to the enhanced sensitivity to Fe deficiency (Tanabe et al 2019). Here, we also confirmed this result (Figure S1).…”

Section: Resultsmentioning

confidence: 99%

“…Although bHLH Ib genes were upregulated and FIT was not affected in the bHLH11-OX plants, the expression of IRT1 and FRO2 was extremely inhibited (Table 1). Recently, Tanabe et al (2019) reported that bHLH11 overexpression inhibits the expression of both FIT and IRT1 /FRO2, and concluded that bHLH11 negatively regulates IRT1 and FRO2 in a FIT-dependent manner (Tanabe et al 2019). However, we did not find the significant response of FIT to bHLH11 overexpression.…”

Section: Discussionmentioning

confidence: 99%

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bHLH11 inhibits bHLH IVc proteins by recruiting the TOPLESS/TOPLESS-RELATED corepressors in Arabidopsis

Yang

Lei

et al. 2020

Preprint

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Iron (Fe) homeostasis is essential for plant growth and development. Although tremendous progress has been made in understanding the maintenance of Fe homeostasis in plants, the underlying molecular mechanisms remain elusive. Recently, bHLH11 was reported to function as a negative regulator. However, the molecular mechanism by which bHLH11 regulates Fe homeostasis is unclear. Here, we generated two bhlh11 loss-of-function mutants which displayed the enhanced sensitivity to excessive Fe. bHLH11 is located in the cytoplasm and nucleus due to lack of a nuclear location signal sequence, and its interaction partners, bHLH IVc transcription factors (TFs) (bHLH34, bHLH104, bHLH105 and bHLH115) facilitate its nuclear accumulation. bHLH11 exerts its negative regulation function by recruiting the corepressors TOPLESS/TOPLESS-RELATED. Moreover, bHLH11 antagonizes the transactivity of bHLH IVc TFs towards bHLH Ib genes (bHLH38, bHLH39, bHLH100 and bHLH101). This work indicates that bHLH11 is a crucial component of Fe homeostasis signaling network, playing a pivotal role in the fine-tuning of Fe homeostasis.

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Section: Resultssupporting

confidence: 49%

“…Recently, it was reported that the overexpression of bHLH11 leads to the enhanced sensitivity to Fe deficiency (Tanabe et al 2019). Here, we also confirmed this result (Figure S1).…”

Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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bHLH11 inhibits bHLH IVc proteins by recruiting the TOPLESS/TOPLESS-RELATED corepressors in Arabidopsis

Yang

Lei

et al. 2020

Preprint

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“…To date, many members in 11 subfamilies (S1-S4, S7, S12, S15, S16, S19, S26, and S27) are involved in plant responses to iron, salt, and drought stresses, and are involved in ABA and JA pathways ( Table 1). For example, members in subfamilies S1, S4, and S12 affect iron uptake [58][59][60]. Studies have also found that AtbHLH17/AIB (S2), AtbHLH92 (S7), AtbHLH112 (S15), and bHLH122 (S27) respond to salt stress [55,[61][62][63], while AtbHLH68 (S15) and…”

Section: The Potential Roles Of Bhlhs In B Napus Rootsmentioning

confidence: 99%

Genome-wide Survey of the bHLH Super Gene Family in Brassica napus

Zhou

et al. 2020

Preprint

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Background: The basic helix-loop-helix (bHLH) gene family is one of the largest transcription factor families in plants and is functionally characterized in diverse species. However, less is known about its functions in the economically important allopolyploid oil crop, Brassica napus . Results : We identified 602 potential bHLHs in the B. napus genome ( BnabHLHs ) and categorized them into 35 subfamilies, including seven newly separated subfamilies, based on phylogeny, protein structure, and exon-intron organization analysis. The intron insertion patterns of this gene family were analyzed and a total of

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“…Until now, many members in 11 subfamilies (S1-S4, S7, S12, S15, S16, S19, S26 and S27) have been identified to response to Fe, salt, and drought stresses, and involve in ABA and JA pathways (Table 1). For example, members in subfamilies S1, S4 and S12 are affected Fe-uptake [56][57][58];…”

Section: The Potential Roles Of Bhlhs In B Napus Rootsmentioning

confidence: 99%

Genome-wide Survey of bHLH Super Gene Family in Brassica napus and Their Roles in Roots

Zhou

et al. 2019

Preprint

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Background: Basic helix-loop-helix (bHLH) gene family is one of the largest transcription factors in plants and are functionally characterized in diverse species. However, less is known about their functions in the economically important allopolyploid oil crop, Brassica napus . Results : We identified 602 potential bHLHs in B. napus genome ( BnbHLHs ) and categorized them into 36 subfamilies, including seven newly separated subfamilies, based on phylogeny, protein structure and exon-intron organization analysis. The intron insertion patterns of this gene family were corrected and a total of

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The basic helix-loop-helix transcription factor, bHLH11 functions in the iron-uptake system in Arabidopsis thaliana

Cited by 61 publications

References 59 publications

bHLH11 inhibits bHLH IVc proteins by recruiting the TOPLESS/TOPLESS-RELATED corepressors in Arabidopsis

bHLH11 inhibits bHLH IVc proteins by recruiting the TOPLESS/TOPLESS-RELATED corepressors in Arabidopsis

Genome-wide Survey of the bHLH Super Gene Family in Brassica napus

Genome-wide Survey of bHLH Super Gene Family in Brassica napus and Their Roles in Roots

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