The best immunohistochemical panel for differentiating hepatocellular carcinoma from metastatic adenocarcinoma

Karabork, Ayca; Kaygusuz, Gülşah; Ekinci, Cemil

doi:10.1016/j.prp.2010.03.004

Cited by 40 publications

(18 citation statements)

References 31 publications

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“…Adenosquamous carcinomas were excluded from the series in order to avoid excessive study complexity. Tumor samples of hepatocellular carcinoma, a tumor type with a well-known immunohistochemical profile[13,14], were included as an internal control group for the semi-supervised evaluation of clustering results.…”

Section: Methodsmentioning

confidence: 99%

Immunohistochemical Typing of Adenocarcinomas of the Pancreatobiliary System Improves Diagnosis and Prognostic Stratification

et al. 2016

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Background & AimsAdenocarcinomas of the pancreatobiliary system are currently classified by their primary anatomical location. In particular, the pathological diagnosis of intrahepatic cholangiocarcinoma is still considered as a diagnosis of exclusion of metastatic adenocarcinoma. Periampullary cancers have been previously classified according to the histological type of differentiation (pancreatobiliary, intestinal), but overlapping morphological features hinder their differential diagnosis. We performed an integrative immunohistochemical analysis of pancreato-biliary tumors to improve their diagnosis and prediction of outcome.MethodsThis was a retrospective observational cohort study on patients with adenocarcinoma of the pancreatobiliary system who underwent diagnostic core needle biopsy or surgical resection at a tertiary referral center. 409 tumor samples were analyzed with up to 27 conventional antibodies used in diagnostic pathology. Immunohistochemical scoring system was the percentage of stained tumor cells. Bioinformatic analysis, internal validation, and survival analysis were performed.ResultsHierarchical clustering and differential expression analysis identified three immunohistochemical tumor types (extrahepatic pancreatobiliary, intestinal, and intrahepatic cholangiocarcinoma) and the discriminant markers between them. Among patients who underwent surgical resection of their primary tumor with curative intent, the intestinal type showed an adjusted hazard ratio of 0.19 for overall survival (95% confidence interval 0.05–0.72; p value = 0.014) compared to the extrahepatic pancreatobiliary type.ConclusionsIntegrative immunohistochemical classification of adenocarcinomas of the pancreatobiliary system results in a characteristic immunohistochemical profile for intrahepatic cholangiocarcinoma and intestinal type adenocarcinoma, which helps in distinguishing them from metastatic and pancreatobiliary type adenocarcinoma, respectively. A diagnostic immunohistochemical panel and additional extended panels of discriminant markers are proposed as guidance for their pathological diagnosis.

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Section: Methodsmentioning

confidence: 99%

Immunohistochemical Typing of Adenocarcinomas of the Pancreatobiliary System Improves Diagnosis and Prognostic Stratification

et al. 2016

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“…Reticulin fibres in the benign processes revealed one-cell thick liver plates, whereas in dysplastic and carcinomatous deposits, they showed thickening of the hepatic cell plates which appeared as two or three cell-cell thick plates instead of usual one-cell thick plates (Figure 4). 3,4 In the present study,18 out of 24 cases of hepatocellular carcinomas stained positive for Alpha Fetoprotein; its sensitivity 84%. 5,6 Thus, Hep Par 1 is more sensitive than Alpha Fetoprotein in the diagnosis of Hepatocellular carcinoma.…”

Section: Figure 4: Well-differentiated Hepatocellular Carcinoma Showisupporting

confidence: 46%

A study of trucut biopsies of hepatic lesions with special reference to immunohistochemistry and special stain

2016

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“…However, CK7 protein is expressed in approximately 1/3 of conventional HCCs33 and CD68 is routinely expressed in macrophages located within the sinusoids of conventional HCCs34. In fact, our analysis shows that at least at the RNA level, many HCCs and CCAs express both CD68 and CK7 at levels observed in FLCs (Supplementary Fig.…”

Section: Resultsmentioning

confidence: 99%

Comprehensive analysis of The Cancer Genome Atlas reveals a unique gene and non-coding RNA signature of fibrolamellar carcinoma

Dinh

Vitucci

Wauthier

et al. 2017

Sci Rep

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Fibrolamellar carcinoma (FLC) is a unique liver cancer primarily affecting young adults and characterized by a fusion event between DNAJB1 and PRKACA. By analyzing RNA-sequencing data from The Cancer Genome Atlas (TCGA) for >9,100 tumors across ~30 cancer types, we show that the DNAJB1-PRKACA fusion is specific to FLCs. We demonstrate that FLC tumors (n = 6) exhibit distinct messenger RNA (mRNA) and long intergenic non-coding RNA (lincRNA) profiles compared to hepatocellular carcinoma (n = 263) and cholangiocarcinoma (n = 36), the two most common liver cancers. We also identify a set of mRNAs (n = 16) and lincRNAs (n = 4), including LINC00473, that distinguish FLC from ~25 other liver and non-liver cancer types. We confirm this unique FLC signature by analysis of two independent FLC cohorts (n = 20 and 34). Lastly, we validate the overexpression of one specific gene in the FLC signature, carbonic anhydrase XII (CA12), at the protein level by western blot and immunohistochemistry. Both the mRNA and lincRNA signatures support a major role for protein kinase A (PKA) signaling in shaping the FLC gene expression landscape, and present novel candidate FLC oncogenes that merit further investigation.

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The best immunohistochemical panel for differentiating hepatocellular carcinoma from metastatic adenocarcinoma

Cited by 40 publications

References 31 publications

Immunohistochemical Typing of Adenocarcinomas of the Pancreatobiliary System Improves Diagnosis and Prognostic Stratification

Immunohistochemical Typing of Adenocarcinomas of the Pancreatobiliary System Improves Diagnosis and Prognostic Stratification

A study of trucut biopsies of hepatic lesions with special reference to immunohistochemistry and special stain

Comprehensive analysis of The Cancer Genome Atlas reveals a unique gene and non-coding RNA signature of fibrolamellar carcinoma

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