The biocontrol by Streptomyces and Trichoderma of leaf spot disease caused by Curvularia oryzae in oil palm seedlings

Sunpapao, Anurag; Chairin, Thanunchanok; S, Itó

doi:10.1016/j.biocontrol.2018.04.017

Cited by 65 publications

(46 citation statements)

References 30 publications

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“…The percentage of muskmelons developing an infection was determined. In addition, each muskmelon was evaluated with a disease score adapted from Sunpapao, Chairin, and Ito (). This score is based on a rating incorporating the extent of invasion of fungal mycelia and fruit rot extent after inoculation: 0 = no invasion, 1 = fungal invasion reaching 0.5 − 1 cm away from the inoculation point, 2 = fungal invasion 1 − 2 cm away, 3 = fungal invasion > 2 cm away, 4 = fungal invasion >2 cm away accompanied by fruit rot (tissue discoloration) beneath the mycelia, and 5 = fungal invasion > 2 cm away accompanied by fruit rot of the whole fruit.…”

Section: Methodsmentioning

confidence: 99%

Fusarium incarnatum is associated with postharvest fruit rot of muskmelon (Cucumis melo)

Wonglom

Sunpapao

2020

Journal of Phytopathology

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Postharvest fruit rot was observed on muskmelon (Cucumis melo) on market shelves at a melon farm in the city of Hatyai, Songkhla, Thailand. Diseased muskmelon fruit displayed cotton‐like mycelia on wounds and had brown to dark brown internal tissue. Based on morphological characteristics and molecular analysis of internal transcribed spacer (ITS) and translation elongation factor 1‐α (TEF1‐α) DNA sequences, the fungal pathogen was identified as Fusarium incarnatum. This species belongs to the Fusarium incarnatum‐equiseti species complex (FIESC). A pathogenicity test was conducted to verify Koch's postulates, and F. incarnatum was observed to cause fruit rot on muskmelon; symptoms of the disease were similar to those seen in the field. However, only artificially wounded melons became infected, suggesting that F. incarnatum is an obligate wound‐infecting pathogen. To our knowledge, this is the first report of F. incarnatum causing fruit rot of muskmelon in Thailand.

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Section: Methodsmentioning

confidence: 99%

Fusarium incarnatum is associated with postharvest fruit rot of muskmelon (Cucumis melo)

Wonglom

Sunpapao

2020

Journal of Phytopathology

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“…Biological control is considered an alternative way to control postharvest fruit rot disease. It is well known that Trichoderma species have many helpful qualities, including antibiosis [ 4 , 5 , 6 , 7 ], competition for nutrients and space [ 8 ], mycoparasitism [ 9 ], and induction of systemic resistance [ 10 , 11 , 12 ] to control disease pathogens, as well as to promote plant growth ability [ 13 ].…”

Section: Introductionmentioning

confidence: 99%

“…Endophytes are a successful biological control agent (BCA) also capable of secreting volatile organic compounds (VOCs) and cell-wall-degrading enzymes to inhibit the fungal growth involved in antibiosis. Some Trichoderma species produce nonvolatile and volatile compounds responsible for inhibiting pathogens [ 4 , 5 , 6 , 16 , 18 , 19 , 20 , 21 ], and may directly trigger the defense system in host plants [ 6 , 11 , 22 ].…”

Section: Introductionmentioning

confidence: 99%

Trichoderma asperellum T76-14 Released Volatile Organic Compounds against Postharvest Fruit Rot in Muskmelons (Cucumis melo) Caused by Fusarium incarnatum

Intana

Kheawleng

Sunpapao

2021

JoF

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Postharvest fruit rot caused by Fusarium incarnatum is a destructive postharvest disease of muskmelon (Cucumis melo). Biocontrol by antagonistic microorganisms is considered an alternative to synthetic fungicide application. The aim of this study was to investigate the mechanisms of action involved in the biocontrol of postharvest fruit rot in muskmelons by Trichoderma species. Seven Trichoderma spp. isolates were selected for in vitro testing against F. incarnatum in potato dextrose agar (PDA) by dual culture assay. In other relevant works, Trichoderma asperellum T76-14 showed a significantly higher percentage of inhibition (81%) than other isolates. Through the sealed plate method, volatile organic compounds (VOCs) emitted from T. asperellum T76-14 proved effective at inhibiting the fungal growth of F. incarnatum by 62.5%. Solid-phase microextraction GC/MS analysis revealed several VOCs emitted from T. asperellum T76-14, whereas the dominant compound was tentatively identified as phenylethyl alcohol (PEA). We have tested commercial volatile (PEA) against in vitro growth of F. incarnatum; the result showed PEA at a concentration of 1.5 mg mL−1 suppressed fungal growth with 56% inhibition. Both VOCs and PEA caused abnormal changes in the fungal mycelia. In vivo testing showed that the lesion size of muskmelons exposed to VOCs from T. asperellum T76-14 was significantly smaller than that of the control. Muskmelons exposed to VOCs from T. asperellum T76-14 showed no fruit rot after incubation at seven days compared to fruit rot in the control. This study demonstrated the ability of T. asperellum T76-14 to produce volatile antifungal compounds, showing that it can be a major mechanism involved in and responsible for the successful inhibition of F. incarnatum and control of postharvest fruit rot in muskmelons.

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“…It has been reported that the VOCs released by Trichoderma spp. play an important role in its antifungal activity (Vinodkumar et al 2017;Sunpapao et al 2018); they can induce systemic resistance (Yi et al 2009) or promote plant growth (Vinale et al 2006). The Trichoderma genus employs several modes of action contributing to biocontrol activity, and these modes vary much depending on the strain and environmental conditions.…”

Section: Vocs Produced By Trichodermamentioning

confidence: 99%

Trichoderma asperellum efficiently protects Quercus robur leaves against Erysiphe alphitoides

Oszako

Войтка²,

Stocki

et al. 2020

Eur J Plant Pathol

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The protective effects of Trichoderma asperellum IZR D-11 as a biocontrol agent against the powdery mildew Erysiphe alphitoides infecting leaves of Quercus robur were evaluated for the first time. A strain of Trichoderma had earlier been isolated in Belarus, and was identified in this study as T. asperellum by sequencing of three genomic markers: internal transcribed spacer, translation elongation factor 1 alpha and RNA polymerase II subunit 2, with over 99.2% identity to corresponding genomic sequences in GenBank. When applied once in the first year just after onset of powdery mildew disease, T asperellum IZR D-11 reduced disease progression and continued to reduce powdery mildew levels during the following three years. Photosynthetic activity as represented by chlorophyll fluorescence measured in oak seedlings was increased in treated plants, and greater assimilate production was also found. The use of this antagonistic fungus increased the total water content in oak leaves suggesting that T. asperellum IZR D-11 can serve as a preventive measure to reduce energy losses in the process of water transpiration. GC-MS analysis detected 49 volatile compounds in the headspace of pure cultures of T. asperellum. Sesquiterpenes represented mainly by daucene, dauca-4(11),8-diene and isodaucene were the largest group of compounds emitted. We speculate that these volatiles from T. asperellum IZR D-11 may be involved in induced resistance in the plant, but further research is needed. The above results suggest that T. asperellum strain IZR D-11 has potential as a biocontrol agent of oak powdery mildew in forest nurseries.

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The biocontrol by Streptomyces and Trichoderma of leaf spot disease caused by Curvularia oryzae in oil palm seedlings

Cited by 65 publications

References 30 publications

Fusarium incarnatum is associated with postharvest fruit rot of muskmelon (Cucumis melo)

Fusarium incarnatum is associated with postharvest fruit rot of muskmelon (Cucumis melo)

Trichoderma asperellum T76-14 Released Volatile Organic Compounds against Postharvest Fruit Rot in Muskmelons (Cucumis melo) Caused by Fusarium incarnatum

Trichoderma asperellum efficiently protects Quercus robur leaves against Erysiphe alphitoides

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