The Bioengineering and Industrial Applications of Bacterial Alkaline Proteases: the Case of SAPB and KERAB

Jaouadi, Bassem; Badis, Abdelmalek; Jaouadi, Nedia Zaraî; Béjar, Samír

doi:10.5772/23850

Cited by 11 publications

(4 citation statements)

References 66 publications

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“…It has been reported that fibrin degradation has been achieved by alkaline fibrinolytic proteases. The use of this fibrinolytic enzyme suggests its future application as an anticancer drug and in thrombolytic therapy (Jaouadi et al, 2011, 2012). Slow-release dosage form preparation containing collagenases with alkaline proteases is extensively used in therapeutic applications.…”

Section: Medical Fieldmentioning

confidence: 99%

Microbial Proteases Applications

Razzaq

Shamsi

Ali³

et al. 2019

Front. Bioeng. Biotechnol.

423

195

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The use of chemicals around the globe in different industries has increased tremendously, affecting the health of people. The modern world intends to replace these noxious chemicals with environmental friendly products for the betterment of life on the planet. Establishing enzymatic processes in spite of chemical processes has been a prime objective of scientists. Various enzymes, specifically microbial proteases, are the most essentially used in different corporate sectors, such as textile, detergent, leather, feed, waste, and others. Proteases with respect to physiological and commercial roles hold a pivotal position. As they are performing synthetic and degradative functions, proteases are found ubiquitously, such as in plants, animals, and microbes. Among different producers of proteases, Bacillus sp. are mostly commercially exploited microbes for proteases. Proteases are successfully considered as an alternative to chemicals and an eco-friendly indicator for nature or the surroundings. The evolutionary relationship among acidic, neutral, and alkaline proteases has been analyzed based on their protein sequences, but there remains a lack of information that regulates the diversity in their specificity. Researchers are looking for microbial proteases as they can tolerate harsh conditions, ways to prevent autoproteolytic activity, stability in optimum pH, and substrate specificity. The current review focuses on the comparison among different proteases and the current problems faced during production and application at the industrial level. Deciphering these issues would enable us to promote microbial proteases economically and commercially around the world.

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Section: Medical Fieldmentioning

confidence: 99%

Microbial Proteases Applications

Razzaq

Shamsi

Ali³

et al. 2019

Front. Bioeng. Biotechnol.

423

195

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“…alone. In fact, a similar study has previously reported the usefulness of alkaline protease, namely SAPB, in the blood and chocolate stain removal from cotton cloth [39]. Leather processing industries generate a lot of toxic pollutants, such as sulfide and chromate, which are found to be detrimental to the environment.…”

Section: Discussionmentioning

confidence: 91%

Effective multi-functional biotechnological applications of protease/keratinase enzyme produced by new Egyptian isolate (Laceyella sacchari YNDH)

Goda

Bassiouny

Monem

et al. 2020

Journal of Genetic Engineering and Biotechnology

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Background Due to a multitude of industrial applications of keratinolytic proteases, their demands are increasing. The present investigation studied the production and monitoring of the most possible multi-functional applications of YNDH thermoalkaline keratin-degrading enzyme. Results This work is considered the first that reported YNDH strain closely related to Laceyella sacchari strain; YNDH is a producer of protease/keratinase enzyme and able to degrade natural keratin such as feathers, wool, human hairs, and nails. Experimental design Plackett-Burman (PBD) was applied to evaluate culture conditions affecting the production of thermoalkaline protease/keratinase. Afterwards, Box-Behnken design (BBD) was applied to find out the optimum level of significant variables namely, NH4Cl, yeast extract, and NaNO3 with a predicted activity of 1324.7 U/ml. Accordingly, the following medium composition and parameters were calculated to be optimum (%w/v): NH4Cl, 0.08; feather, 1; yeast extract, 0.04; MgSO4.7H2O, 0.02; NaNO3, 0.016; KH2PO4, 0.01; K2HPO4, 0.01; pH, 8; inoculum size; 5%, cultivation temperature (Temp.) 45 °C and incubation time 48 h. The studied enzyme can degrade keratin-azure, remove proteinaceous materials, and is able to remove hairs from goat hides. These interesting characteristics make this enzyme a good candidate in many applications especially in detergent (Det.), in leather industries, and in pharmaceuticals particularly in nail treatment. Conclusion The promising properties of the newly keratin-degrading protease enzyme from Laceyella sacchari strain YNDH would underpin its efficient exploitation in several industries to cope with the demands of worldwide enzyme markets.

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“…Enzymes are macromolecular biological catalysts (Stryer, 2002). Their choice over chemicals in the improvement of efficiency and reduction of cost in different industrial processes and systems has been in process for a long time (Jaouadi et al, 2011). Proteases have a wide distribution in nature and are particularly important in their ability to hydrolyse peptide bonds in aqueous environments (Jaouadi et al, 2011).…”

Section: Introductionmentioning

confidence: 99%

“…Their choice over chemicals in the improvement of efficiency and reduction of cost in different industrial processes and systems has been in process for a long time (Jaouadi et al, 2011). Proteases have a wide distribution in nature and are particularly important in their ability to hydrolyse peptide bonds in aqueous environments (Jaouadi et al, 2011). Keratinase are a group of proteases with a wide temperature and pH range that allow complete degradation of complex and recalcitrant proteins such a keratin (Vidmar and Vodovnik 2018).…”

Section: Introductionmentioning

confidence: 99%

Hair Removal Potential of Keratinase Produced by Bacillus Species Isolated From Feather Waste

DADA

Wakil²

2021

J microb biotech food sci

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Keratins are tough, fibrous proteins found as the major protein component in hair. Chemical hair removal is harsh and results in severe toxicity and pollution, hence, a need for a non-chemical enzymatic hair removal process. The aim of this study is to characterise keratinase from isolated keratin-degrading Bacillus licheniformis-K51, Bacillus subtilis-K50 and Bacillus sp.-K53 and investigate its potential in hair removal. The collagenolytic ability of keratin-degrading keratinases obtained from Bacillus licheniformis-K51, Bacillus subtilis-K50 and Bacillus sp.-K53 was determined using standard methods. The depilatory potential of keratinase (directly and as part of the constituent of formulated depilatory cream) was determined in vitro on goat skin and in vivo on Wistar rat. Purified keratinases from Bacillus licheniformis-K51 (EZYKer-51), Bacillus subtilis-K50 (EZYKer-50) and Bacillus sp.-K53 (EZYKer-53) did not show significant collagenolytic activities. In vitro treatment of goat skin with EZYKer-51 showed total hair removal within 24 hours and partial hair removal with EZYKer-50 and EZYKer-53. The formulated depilatory cream containing EZYKer-51 showed highest keratinolytic activity (22.20±0.63 U/mL) followed by EZYKer-50 (20.52±0.46 U/mL) and EZYKer-53 (17.06±0.81 U/mL). In vivo depilatory action using formulated cream took 40 min, while that of commercial cream was achieved within 20 min. Keratinase from Bacillus licheniformis-K51 exhibited complete hair-removal function which makes it potentially suitable in industrial processes involving removal of hair. ARTICLE INFO

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The Bioengineering and Industrial Applications of Bacterial Alkaline Proteases: the Case of SAPB and KERAB

Cited by 11 publications

References 66 publications

Microbial Proteases Applications

Microbial Proteases Applications

Effective multi-functional biotechnological applications of protease/keratinase enzyme produced by new Egyptian isolate (Laceyella sacchari YNDH)

Hair Removal Potential of Keratinase Produced by Bacillus Species Isolated From Feather Waste

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