The Bioinformatical Identification of Potential Biomarkers in Heart Failure Diagnosis and Treatment

Abstract

Background. Heart failure (HF) is defined as the inability of the heart’s systolic and diastolic function to properly discharge blood flow from the veins to the heart. The goal of our research is to look into the possible mechanism that causes HF. Methods. The GSE5406 database was used for screening the differentially expressed genes (DEGs). Gene ontology (GO), Kyoto Encyclopedia of Genes and Genomes (KEGG), and Protein-Protein Interaction (PPI) network were applied to analyze DEGs. Besides, cell counting Kit-8 (CCK-8) was conducted to observe the knockdown effect of hub genes on cell proliferation. Results. Finally, 377 upregulated and 461 downregulated DEGs came out, enriched in the extracellular matrix organization and gap junction. According to GSEA results, Hoft cd4 positive alpha beta memory t cell bcg vaccine age 18–45 yo id 7 dy top 100 deg ex vivo up, Sobolev t cell pandemrix age 18–64 yo 7 dy dn, and so on were significantly related to gene set GSE5406. 7 hub genes, such as COL1A1, UBB, COL3A1, HSP90AA1, MYC, STAT3 and MAPK1, were selected from PPI networks. CCK-8 indicated silencing of STAT3 promoted the proliferation of H9C2 cells and silencing of UBB inhibited the proliferation of H9C2 cells. Conclusion. Our analysis reveals that COL1A1, UBB, COL3A1, HSP90AA1, MYC, STAT3, and MAPK1 might promote the progression of HF and become the biomarkers for diagnosis and treatment of HF.

Exploring the mechanism of Tingli Pill in the treatment of HFpEF based on network pharmacology and molecular docking

“…MAPK family is a kind of serine/threonine kinase, and also participates in stress reaction processes such as inflammation, cell hypertrophy, proliferation, and apoptosis. Among them, MAPK1 is a biomarker of heart failure, which is closely related to the occurrence and development of heart failure; [31] MAPK14 is involved in the process of energy metabolism related to heart failure. [32] TP53 is an important tumor suppressor gene in the human body.…”

Identification of shared molecular mechanisms and diagnostic biomarkers between heart failure and idiopathic pulmonary fibrosis

“…The COL1A1 gene expression product is the primary component of the ECM, and research has demonstrated its myocardial protective impact on HF [ 23 ]. Additionally, it has been recognized as a potential biomarker for the advancement of HF, specifically in predicting the progression from HF diagnosis to the survival rate one year after transplantation [ 23 , 24 ]. The COL3A1 gene encodes for the synthesis of the collagen alpha-1(III) chain.…”

“…A recent study suggested that CCND1 may serve as an underlying biomarker of idiopathic dilated cardiomyopathy [ 38 ]. In fact, idiopathic dilated cardiomyopathy is one of the most significant triggers of HF, and its histological manifestations include cardiomyocyte hypertrophy, interstitial fibrosis, and myofibril loss [ 24 , 39 ].…”

DNA Methylation Analysis Identifies Novel Epigenetic Loci in Dilated Murine Heart upon Exposure to Volume Overload

“…SPCS3 belongs to the signal peptidase complex, which cleaves signal peptides from ER-targeted faulty proteins and thus maintains a healthy membrane proteome [ 53 ]. Spcs3 was reported to be differentially expressed in ischemic human and murine cardiomyopathy and in pressure-overloaded hearts [ 35 , 54 , 55 ]. Future loss- and gain-of-function studies are required to determine whether these identified candidates have a detrimental effect on the pathological remodeling and HF progression upon VO exposure or whether they are indeed protective.…”