The blocking of human antibody-dependent, eosinophil-mediated killing of<i>Schistosoma mansoni</i>schistosomula by monoclonal antibodies which cross-react with a polysaccharide-containing egg antigen

Dunne, DW; Qd, Bickle; Butterworth, A E; Ba, Richardson

doi:10.1017/s0031182000053944

Cited by 45 publications

(23 citation statements)

References 26 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…These authors observed that et al (1985). Further studies on Ig isotypes secreted around juvenile flukes are necessary to explore this antibody-blocking evasion mechanism, which has also been described in schistosomes (Capron et al, 1987;Dunne et al 1987). While F. hepatica seems able to evade ADCC mechanisms, which may be regulated by Th2 cytokines, further studies are needed to explore the role of the parasite in regulation of the immune response.…”

Section: Hepatic Lymph Nodementioning

confidence: 97%

Local immune response to experimentalFasciola hepaticainfection in sheep

Chauvin

Boulard²

1996

Parasite

View full text Add to dashboard Cite

Summary :Macrophages, eosinophils, neutrophils and lymphocyte subpopulations (OvCD5+, OvCD4+, OvCD8+, OvWC1+ and lg + ) were identified in sections of hepatic tissue and hepatic lymph nodes from sheep experimentaly infected with F. hepatica and necropsied 14, 28, 42 or 56 days post infection. The migratory tunnels produced by juvenile flukes appeared as focal areas of necrosis surrounded by infiltrating inflammatory cells, particularly numerous macrophages, eosinophils and OvCD4 + lymphocytes. In addition, B lymphocyte proliferation was observed in hepatic lymph nodes and in hepatic portal tracts. Only three juvenile flukes were identified in the sections. They where partially in contact with healthy tissue and partially with neutrophils, macrophages and eosinophils; they were covered by IgM. Host-parasite interactions resulting from immune response regulation by helper T lymphocytes and from immune evasion by the parasite are discussed.

show abstract

Section: Hepatic Lymph Nodementioning

confidence: 97%

Local immune response to experimentalFasciola hepaticainfection in sheep

Chauvin

Boulard²

1996

Parasite

View full text Add to dashboard Cite

show abstract

“…Initially, two different K3 ELISA assays were compared. The first involved the use of a mouse monoclonal IgM antibody (mAb), M22GllP, which blocks eosinophil killing of schistosomula [17], bound to ELISA plates in order to capture K3 from SEA. The second method employed the same IgM mAb, covalently linked to Sepharose beads, to immunoaffinity purify the K3 antigen from SEA.…”

Section: Antigensmentioning

confidence: 99%

“…Cultured schistosomular shed antigen (Shed.SA) was recovered from the supernatant fluid collected from an 18-h culture ELISA assays were also carried out using the polysaccharide egg antigen K3, which had been immunoaffinity purified from SEA using purified mouse IgM anti-K3 mAb, M22GllP [17] covalently linked to CNBr-activated Sepharose. This assay was compared with a second K3 ELISA assay in which the same mAb, which had been highly purified by gel filtration HPLC, was coated directly on microtiter plates overnight.…”

Section: Antigensmentioning

confidence: 99%

Human antibody responses to Schistosoma mansoni: the influence of epitopes shared between different life‐cycle stages on the response to the schistosomulum

et al. 1988

View full text Add to dashboard Cite

Sera from 120 Kenyan schoolchildren who were infected with S. mansoni were individually examined, using an enzyme-linked immunoabsorbent assay (ELISA), for the presence of IgG and IgM antibodies reactive with antigens derived from adult worms, the outer membrane of the schistosomulum or from the parasite egg. In addition, antibodies against more purified egg antigens, an egg stage-specific glycoprotein preparation and a polysaccharide egg antigen known to share epitopes with the schistosomular surface were measured in ELISA, as were antibodies reactive with trichloroacetic acid-soluble and periodate-insensitive antigens derived from the outer membrane of schistosomulum and antigens shed when schistosomula were cultured in vitro. IgG subclass responses to the unfractionated egg antigen were also measured. The results from each of these assays were compared with the results of each other assay and with the number of parasite eggs excreted by each child, using Spearmans rank correlations. These comparisons revealed a number of statistically significant positive correlations. IgG4 anti-egg antibodies correlated better with intensity of infection than did other IgG subclasses. Total IgG responses against polysaccharide antigens did not correlate with intensity of infection as well as IgG responses against other antigens; epitopes shared between the schistosomulum surface and the adult worm were different to those shared with the parasite egg; and, there was antigen-directed restriction of IgG subclass responses to some egg and adult worm antigens which carried these shared epitopes. It is argued that this might have a qualitative effect on the nature of the antibodies directed against the schistosomulum by infected individuals and therefore have important consequences for the outcome of a subsequent exposure to infection with the same parasite.

show abstract

“…The diirerenl helminthiasis are among the most prevalent of chronic infections of humans, and numerous studies have demonstrated thai there is a process of constant reinfection in endemic areas [1,2], One of the most interesting features of the immtme response to these parasites is the produetion of IgE antibodies [3], and there exists considerable evidence that ihe IgE response may participate in the protection against helminthic infection [4][5][6][7]. For example, the allergic-type reactions mediated by the interaction beiween IgE and mast cells may be important accessory mechanisms in helminih expulsion [8].…”

Section: Introductionmentioning

confidence: 99%

Ascaris reinfection of slum children: relation with the IgE response

Hagel

Lynch

Prisco

et al. 1993

Clinical and Experimental Immunology

View full text Add to dashboard Cite

SUMMARYTolal and ^,«-flm-specific serum IgE levels vi^ere measured in a group of 98 Ascaris-infcdcii children from a slum area of Caracas. Venezuela, in whom the infections were eliminated by regular treatment for 22 months with ihe anthelmint O.xantei Pyrantel ('Quanlrel"), The children were re-evalualed at the end of the treatment programme, and then 8 months later, al which lime reinfection was assessed. Total IgE levels at the beginning of the study were significanlly higher in the children who became reinfected after trealmenl. compared with those who did not. The anihclmint treatment caused a significant decrease in Ihe tolal IgE levels in mosl of the children, and afler a period of 8 months without treatment these continued lo decrea.se in the non-reinfected group, bul increased again in Ihe reinfected children. The reverse pattern was found for .'J.vct/r/.v-specific igE antibody levels, and in fad an inverse correlation was found between total and anli-Ascaris IgE levels. Striking associations were found between reinfection and high pretreatment values of lotal IgF.. but low levels of specific IgE antibody. These data support the concept that specific IgE antibody may participale in the protection against helminthic infection, and suggest that Ihe polyclonal stimulation of IgE synthesis caused by these parasites may reduce iheeffeetivenessofsuch responses. The results also indicate that different individuals have varying propensities to respond polyclonally to the helminths, and this influences their resistance lo infeclion.

show abstract

The blocking of human antibody-dependent, eosinophil-mediated killing ofSchistosoma mansonischistosomula by monoclonal antibodies which cross-react with a polysaccharide-containing egg antigen

Cited by 45 publications

References 26 publications

Local immune response to experimentalFasciola hepaticainfection in sheep

Local immune response to experimentalFasciola hepaticainfection in sheep

Human antibody responses to Schistosoma mansoni: the influence of epitopes shared between different life‐cycle stages on the response to the schistosomulum

Ascaris reinfection of slum children: relation with the IgE response

Contact Info

Product

Resources

About