The bovine embryo hatches from the zona pellucida through either the embryonic or abembryonic pole

Negrón‐Pérez, Verónica M.; Hansen, Peter J.

doi:10.1007/s10815-017-0933-3

Cited by 10 publications

(14 citation statements)

References 28 publications

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“…In humans, a previous study showed that fresh blastocysts usually hatch from the site opposite the ICM, although a few choose to hatch near the ICM or elsewhere 13 ; however, the correlation between hatching site and birth outcome was not evaluated. Conversely, another study employed fresh bovine embryos and showed that there was nearly an equal probability that hatching would occur ipsilateral or contralateral to the ICM 14 . Combined with our results on blastocyst hatching site inclination, we presume that different species have variable preferences in terms of blastocyst hatching site.…”

Section: Discussionmentioning

confidence: 99%

Blastocyst hatching site is regularly distributed and does not influence foetal development in mice

Liu

Sun

Hao

et al. 2020

Sci Rep

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Hatching out from the zona pellucida (Zp) is a crucial step for blastocyst implantation and development. However, it is still unknown whether the location of the hatching site relative to the inner cell mass (ICM) affects embryo implantation and foetal development. Here, we classified hatching blastocysts into three categories, 0° ≤ θ ≤ 30°, 30° < θ ≤ 60°, and 60° < θ ≤ 90°, in which θ is determined based on the relative position of the hatching site to the arc midpoint of the icM. non-surgical embryo transfer (NSET) devices were employed to evaluate blastocyst implantation and embryo development. Of 1,827 hatching blastocysts, 43.84%, 30.60%, and 21.67% were categorized as 30° < θ ≤ 60°, 0° ≤ θ ≤ 30°, and 60° < θ ≤ 90°, respectively. Embryos with different hatching sites showed no distinct differences in blastocyst implantation; surrogate female pregnancy; embryo development to term; litter size, or offspring survival, gender, or body weight. Our results indicate that mouse blastocyst hatching site is not randomly distributed. embryo implantation and development are not correlated with the blastocyst hatching site in mice. thus, assessment of the blastocyst hatching site should not be recommended to evaluate mouse blastocyst implantation and developmental potential.

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Section: Discussionmentioning

confidence: 99%

Blastocyst hatching site is regularly distributed and does not influence foetal development in mice

Liu

Sun

Hao

et al. 2020

Sci Rep

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“…Likewise, in humans, various authors have reported the formation of natural hatching sites close to the ICM [42,48] as well as opposite to the ICM [49,50]. For bovine embryos, nearly equal probability of hatching from the embryonic or abembryonic poles has been demonstrated [51]. However, while the issue of the possible influence of the AH site on the hatching rate, blastocyst implantation, and embryo development remains controversial [43,46,47] and requires further study, the proposed technique enables safe, quick, and reliable ZP drilling at both abembryonic and embryonic poles and thus seems to be very useful for improving the AH effectiveness and overall embryo development.…”

Section: Discussionmentioning

confidence: 92%

Controlled hatching at the prescribed site using femtosecond laser for zona pellucida drilling at the early blastocyst stage

Ilina

Khramova

Ivanova

et al. 2020

J Assist Reprod Genet

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Purpose To study whether the application of femtosecond laser pulses for zona pellucida (ZP) drilling of blastocysts at the embryonic or abembryonic poles can promote hatching to start immediately through the hole formed and ensure high hatching rates and embryo viability. Methods Mouse blastocyst (E3.5) ZP were microdissected with femtosecond laser pulses (514-nm wavelength, 280-fs pulse duration, 2.5-kHz repetition rate) close to the trophoblast or inner cell mass (ICM). The sizes of the holes formed were in the range of 4.5-8.5 μm. Additional longitudinal incisions (5-7-μm long) on either side of the hole were created to determine whether hatching had started at the correct position. Embryos post-laser-assisted ZP drilling and intact embryos were cultured under standard conditions for 2 days; embryo quality was assessed twice daily. The hatching rates and in vitro and in vivo implantation rates (only for embryos with ZP dissected close to the ICM) were estimated. Results Femtosecond laser-assisted ZP drilling at the early blastocyst stage facilitated embryo hatching to start at the artificial opening with probability approaching 100%. Despite the artificial opening's small size, no embryo trapping during hatching was observed. Both experimental groups had higher hatching rates than the control groups (93.3-94.7% vs. 83.3-85.7%, respectively). The in vitro implantation rate was comparable with that of the control group (92.3% vs. 95.4%). No statistically significant differences were obtained in the in vivo implantation rates between the experimental and control groups. Conclusions Blastocyst-stage femtosecond laser microsurgery of ZP is fast and delicate and enables the hatching process to be initiated in a controlled manner through a relatively small opening, with no embryo trapping.

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“…Cells that were TE were distinguished from cells in the ICM based on nuclear labeling for CDX2 using procedures similar to those previously published [4, 25, 44, 45]. The primary antibody was ready-to-use mouse monoclonal antibody against CDX2 (BioGenex, Fremont, CA, USA; reference AM392-5 M, lot AM3920917) For randomly-selected embryos, mouse 1gG (1 μg/mL) was used as a negative control.…”

Section: Methodsmentioning

confidence: 99%

Importance of prostate androgen-regulated mucin-like protein 1 in development of the bovine blastocyst

2019

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Background Prostate androgen-regulated mucin-like protein 1 (PARM1) is a pro-proliferative and anti-apoptotic glycoprotein involved in the endoplasmic reticulum (ER) stress response. A single nucleotide polymorphism in the coding region of PARM1 has been associated with competence of bovine embryos to develop to the blastocyst stage. Here we tested the importance of PARM1 for development by evaluating consequences of reducing PARM1 mRNA abundance on embryonic development and differentiation, gene expression and resistance to ER stress. Results Knockdown of PARM1 using an anti- PARM1 GapmeR did not affect competence of embryos to develop into blastocysts but decreased the number of trophectoderm (TE) cells in the blastocyst and tended to increase the number of cells in the blastocyst inner cell mass (ICM). Treatment of embryos with anti- PARM1 GapmeR affected expression of 4 and 3 of 90 genes evaluated at the compact-morula and blastocyst stage of development at days 5.5 and 7.5 after fertilization, respectively. In morulae, treatment increased expression of DAB2, INADL, and STAT3 and decreased expression of CCR2 . At the blastocyst stage, knockdown of PARM1 increased expression of PECAM and TEAD4 and decreased expression of CCR7. The potential role of PARM1 in ER stress response was determined by evaluating effects of knockdown of PARM1 on development of embryos after exposure to heat shock or tunicamycin and on expression of ATF6 , DDIT3 and EIF2AK3 at the compact morula and blastocyst stages. Both heat shock and tunicamycin reduced the percent of embryos becoming a blastocyst but response was unaffected by PARM1 knockdown. Similarly, there was no effect of knockdown on steady-state amounts of ATF6 , DDIT3 or EIF2AK3. Conclusion PARM1 participates in formation of TE and ICM cells in early embryonic development but there is no evidence for the role of PARM1 in the ER stress response. Electronic supplementary material The online version of this article (10.1186/s12861-019-0195-7) contains supplementary material, which is available to authorized users.

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The bovine embryo hatches from the zona pellucida through either the embryonic or abembryonic pole

Cited by 10 publications

References 28 publications

Blastocyst hatching site is regularly distributed and does not influence foetal development in mice

Blastocyst hatching site is regularly distributed and does not influence foetal development in mice

Controlled hatching at the prescribed site using femtosecond laser for zona pellucida drilling at the early blastocyst stage

Importance of prostate androgen-regulated mucin-like protein 1 in development of the bovine blastocyst

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