The bZIP transcription factor TabZIP15 improves salt stress tolerance in wheat

Bi, Chenxi; Yu, Yuehua; Dong, Caixia; Yang, Yuxin; Zhai, Yiqian; Du, Fengping; Xia, Chuan; Ni, Zhongfu; Kong, Xiuying; Zhang, Lichao

doi:10.1111/pbi.13453

Cited by 75 publications

(33 citation statements)

References 8 publications

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“…The bZIP transcription factor plays a crucial role in plant growth, development, and abiotic stress responses, such as seed maturation, flower development, stress response, ect [ 25 , 26 , 33 ]. Currently, the bZIP gene family has been studied in the model plant, especially for Arabidopsis and crops, such as AtbZIP11/18 in Arabidopsis [ 27 , 29 ], GsbZIP67 in soybeans [ 32 ], CabZIP25 in pepper [ 33 ], TabZIP15 in wheat [ 34 ] and MdHY5 / MdbZIP44 in apple [ 40 , 43 ]. Until now, despite the sequencing of the whole pomegranate gene has been completed, little is known about the bZIP gene family in pomegranate [ 45 , 47 , 48 ].…”

Section: Discussionmentioning

confidence: 99%

“…In addition, the bZIP genes also play an important role in plant biotic and abiotic stress [ 31 – 33 ]. In wheat, TabZIP15 promotes the combination of ABF/AREB and ABRE (ABA response element) cis-acting elements through the expression of ABF/AREB to induce downstream target gene expression responding to plant salt and drought stress [ 34 ]. Similar results were observed for the GsbZIP67 gene in Alfalfa ( Medicago sativa ), overexpression of GsbZIP67 promoted the growth of plant roots and shoots and changed the physiological indicators of transgenic plants under bicarbonate salt-alkali stress [ 32 ].…”

Section: Introductionmentioning

confidence: 99%

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Genome-wide identification and characterization of bZIP gene family and cloning of candidate genes for anthocyanin biosynthesis in pomegranate (Punica granatum)

Wang

Zhang

et al. 2022

BMC Plant Biol

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Background The basic leucine zipper (bZIP) transcription factor is one of the most abundant and conserved gene families in eukaryotes. In addition to participating in plant development and growth, bZIP transcription factors play crucial roles in various abiotic stress responses and anthocyanin accumulation. Up to now, analysis of bZIP gene family members in pomegranate (Punica granatum) has not been reported. Three published pomegranate genome sequences provide valuable resources for further gene function analysis. Results Using bioinformatics analysis, 65 PgbZIPs were identified and analyzed from the ‘Taishanhong’ pomegranate genome. We divided them into 13 groups (A, B, C, D, E, F, G, H, I, J, K, M, and S) according to the phylogenetic relationship with those of Arabidopsis, each containing a different number of genes. The regularity of exon/intron number and distribution was consistent with the classification of groups in the evolutionary tree. Transcriptome analysis of different tissues showed that members of the PgbZIP gene family were differentially expressed in different developmental stages and tissues of pomegranate. Among them, we selected PgbZIP16 and PgbZIP34 as candidate genes which affect anthocyanin accumulation. The full-length CDS region of PgbZIP16 and PgbZIP34 were cloned from pomegranate petals by homologous cloning technique, encoding 170 and 174 amino acids, which were 510 bp and 522 bp, respectively. Subcellular localization assays suggested that both PgbZIP16 and PgbZIP34 were nucleus-localized. Real-time quantitative PCR (qPCR) was used to explore the expression of PgbZIP16 and PgbZIP34 in the petals of three kinds of ornamental pomegranates at the full flowering stage. The results demonstrated that the expression of PgbZIP16 in red petals was 5.83 times of that in white petals, while PgbZIP34 was 3.9 times. The results of transient expression in tobacco showed that consistent trends were observed in anthocyanin concentration and expression levels of related genes, which both increased and then decreased. Both PgbZIP16 and PgbZIP34 could promote anthocyanin accumulation in tobacco leaves. We obtained transgenic strains overexpressing PgbZIP16, and the histochemical staining for GUS activity showed that overexpressed PgbZIP16 seedlings were expressed in the stem. Transgenic experiments indicated that overexpression of PgbZIP16 significantly upregulated UF3GT, ANS and DFR genes in Arabidopsis and enhanced anthocyanin accumulation. Conclusions The whole genome identification, gene structure, phylogeny, gene cloning, subcellular location and functional verification of the pomegranate bZIP gene family provide a theoretical foundation for the functional study of the PgbZIP gene family and candidate genes for anthocyanin biosynthesis.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Genome-wide identification and characterization of bZIP gene family and cloning of candidate genes for anthocyanin biosynthesis in pomegranate (Punica granatum)

Wang

Zhang

et al. 2022

BMC Plant Biol

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“…As a bZIP transcription factor, TaABP1 had better drought resistance and was strongly expressed under ABA treatment [28]. TabZIP15 can improve salt tolerance of wheat [29]. TaFDL2 interacts with TabZIP8 protein to jointly regulate ABA signaling in response to drought stress [30].…”

Section: Introductionmentioning

confidence: 99%

Genome-Wide Identification and Analysis of bZIP Gene Family and Resistance of TaABI5 (TabZIP96) under Freezing Stress in Wheat (Triticum aestivum)

Liang

Xia

Jiang

et al. 2022

IJMS

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The basic leucine zipper (bZIP) regulates plant growth and responds to stress as a key transcription factor of the Abscisic acid (ABA) signaling pathway. In this study, TabZIP genes were identified in wheat and the gene structure, physicochemical properties, cis-acting elements, and gene collinearity were analyzed. RNA-Seq and qRT-PCR analysis showed that ABA and abiotic stress induced most TabZIP genes expression. The ectopic expression of TaABI5 up-regulated the expression of several cold-responsive genes in Arabidopsis. Physiological indexes of seedlings of different lines under freezing stress showed that TaABI5 enhanced the freezing tolerance of plants. Subcellular localization showed that TaABI5 is localized in the nucleus. Furthermore, TaABI5 physically interacted with cold-resistant transcription factor TaICE1 in yeast two-hybrid system. In conclusion, this study identified and analyzed members of the TabZIP gene family in wheat. It proved for the first time that the gene TaABI5 affected the cold tolerance of transgenic plants and was convenient for us to understand the cold resistance molecular mechanism of TaABI5. These results will provide a new inspiration for further study on improving plant abiotic stress resistance.

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“…Within this gene regulatory network, the plant response to high salt stress involves a variety of signal transduction pathways, including two main ones: an abscisic acid (ABA)-dependent pathway and an ABA-independent pathway. In the ABA-dependent pathway, many TFs, such as MYB (Dubos et al, 2010;Li J. et al, 2019 andZhao et al, 2019), bZIP (Zhang M. et al, 2020;Bi et al, 2021), NAC (Joshi et al, 2016;Khedia et al, 2018;Li et al, 2021; TFs, which have ABA-responsive element-binding factors in their promoter regions, play an important role in the plant response to salt stress. For example, Arabidopsis NAC069 mutants are insensitive to salt and cold stresses (He et al, 2017).…”

Section: Introductionmentioning

confidence: 99%

Genome-Wide Association Analysis Coupled With Transcriptome Analysis Reveals Candidate Genes Related to Salt Stress in Alfalfa (Medicago sativa L.)

Wei

Zhang

et al. 2022

Front. Plant Sci.

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Salt stress is the main abiotic factor affecting alfalfa yield and quality. However, knowledge of the genetic basis of the salt stress response in alfalfa is still limited. Here, a genome-wide association study (GWAS) involving 875,023 single-nucleotide polymorphisms (SNPs) was conducted on 220 alfalfa varieties under both normal and salt-stress conditions. Phenotypic analysis showed that breeding status and geographical origin play important roles in the alfalfa salt stress response. For germination ability under salt stress, a total of 15 significant SNPs explaining 9%–14% of the phenotypic variation were identified. For tolerance to salt stress in the seedling stage, a total of 18 significant SNPs explaining 12%–23% of the phenotypic variation were identified. Transcriptome analysis revealed 2,097 and 812 differentially expressed genes (DEGs) that were upregulated and 2,445 and 928 DEGs that were downregulated in the leaves and roots, respectively, under salt stress. Among these DEGs, many encoding transcription factors (TFs) were found, including MYB-, CBF-, NAC-, and bZIP-encoding genes. Combining the results of our GWAS analysis and transcriptome analysis, we identified a total of eight candidate genes (five candidate genes for tolerance to salt stress and three candidate genes for germination ability under salt stress). Two SNPs located within the upstream region of MsAUX28, which encodes an auxin response protein, were significantly associated with tolerance to salt stress. The two significant SNPs within the upstream region of MsAUX28 existed as three different haplotypes in this panel. Hap 1 (G/G, A/A) was under selection in the alfalfa domestication and improvement process.

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The bZIP transcription factor TabZIP15 improves salt stress tolerance in wheat

Cited by 75 publications

References 8 publications

Genome-wide identification and characterization of bZIP gene family and cloning of candidate genes for anthocyanin biosynthesis in pomegranate (Punica granatum)

Genome-wide identification and characterization of bZIP gene family and cloning of candidate genes for anthocyanin biosynthesis in pomegranate (Punica granatum)

Genome-Wide Identification and Analysis of bZIP Gene Family and Resistance of TaABI5 (TabZIP96) under Freezing Stress in Wheat (Triticum aestivum)

Genome-Wide Association Analysis Coupled With Transcriptome Analysis Reveals Candidate Genes Related to Salt Stress in Alfalfa (Medicago sativa L.)

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