The C. elegans lifespan assay toolkit

Amrit, Francis Raj Gandhi; Ratnappan, Ramesh; Keith, Scott A.; Ghazi, Arjumand

doi:10.1016/j.ymeth.2014.04.002

Cited by 112 publications

(107 citation statements)

References 74 publications

Supporting

Mentioning

107

Contrasting

Order By: Relevance

“…Then, worms were transferred onto plates with RNAi clones. 96 animals were used per condition and scored every day or every other day74. From the initial worm population, the worms that are lost or burrow into the medium as well as those that exhibit ‘protruding vulva' or undergo bagging were censored.…”

Section: Methodsmentioning

confidence: 99%

Somatic increase of CCT8 mimics proteostasis of human pluripotent stem cells and extends C. elegans lifespan

et al. 2016

View full text Add to dashboard Cite

Human embryonic stem cells can replicate indefinitely while maintaining their undifferentiated state and, therefore, are immortal in culture. This capacity may demand avoidance of any imbalance in protein homeostasis (proteostasis) that would otherwise compromise stem cell identity. Here we show that human pluripotent stem cells exhibit enhanced assembly of the TRiC/CCT complex, a chaperonin that facilitates the folding of 10% of the proteome. We find that ectopic expression of a single subunit (CCT8) is sufficient to increase TRiC/CCT assembly. Moreover, increased TRiC/CCT complex is required to avoid aggregation of mutant Huntingtin protein. We further show that increased expression of CCT8 in somatic tissues extends Caenorhabditis elegans lifespan in a TRiC/CCT-dependent manner. Ectopic expression of CCT8 also ameliorates the age-associated demise of proteostasis and corrects proteostatic deficiencies in worm models of Huntington's disease. Our results suggest proteostasis is a common principle that links organismal longevity with hESC immortality.

show abstract

Section: Methodsmentioning

confidence: 99%

Somatic increase of CCT8 mimics proteostasis of human pluripotent stem cells and extends C. elegans lifespan

et al. 2016

View full text Add to dashboard Cite

show abstract

“…Detailed methods for lifespan assays using solid medium systems are described in another review article (Amrit et al, 2014), but here, we will discuss the methods very briefly. First, synchronization of the specific developmental stages of worms is required.…”

Section: Lifespan Assaysmentioning

confidence: 99%

“…A general method for determining death is counting worms that do not respond to a gentle touch with a sterilized platinum wire. Alternatively, the live status of worms can be determined by observing avoidance from a heated platinum wire located close to their heads (Amrit et al, 2014). Another method is executed by flooding many replica plates with water at each time point and counting animals responsive to a gentle touch to the surface of water with a platinum wire (Samuelson et al, 2007).…”

Section: Lifespan Assaysmentioning

confidence: 99%

“…Before synchronization, eggs are collected from gravid adults by using a method called bleaching, which can also be used for large-scale, solid culture lifespan assays (Amrit et al, 2014). During bleaching, gravid adults are treated with sodium hypochlorite and washed with M9 buffer, a widely used phosphate-based buffer for C. elegans research.…”

Section: Lifespan Assaysmentioning

confidence: 99%

“…Standard temperatures for survival assays range from 15°C to 25°C, though 20°C is the most widely used temperature. Exact environmental conditions for survival assays should be determined depending on the specific features of the survival assays (Amrit et al, 2014; Keith et al, 2014). Here, we briefly discuss the methods, requirements, advantages, and disadvantages of different survival assays using C. elegans , such as lifespan assays crucial for aging research, various abiotic stress resistance assays, and pathogen resistance assays important for studying immunity.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Survival assays using Caenorhabditis elegans

Park

Jung

Lee

2017

Molecules and Cells

132

View full text Add to dashboard Cite

Caenorhabditis elegans is an important model organism with many useful features, including rapid development and aging, easy cultivation, and genetic tractability. Survival assays using C. elegans are powerful methods for studying physiological processes. In this review, we describe diverse types of C. elegans survival assays and discuss the aims, uses, and advantages of specific assays. C. elegans survival assays have played key roles in identifying novel genetic factors that regulate many aspects of animal physiology, such as aging and lifespan, stress response, and immunity against pathogens. Because many genetic factors discovered using C. elegans are evolutionarily conserved, survival assays can provide insights into mechanisms underlying physiological processes in mammals, including humans.

show abstract

Expanding the Repertoire of ceDAF‐12 Ligands for Modulation of the Steroid Endocrine System in C. Elegans

Galilea,

Santillán,

Acebedo

et al. 2024

ChemBioChem

View full text Add to dashboard Cite

Steroid hormones are essential for the biological processes of eukaryotic organisms. The steroid endocrine system of C. elegans, which includes dafachronic acids (DA) and the nuclear receptor ceDAF‐12, provides a simple model for exploring the role of steroid hormone signaling pathways in animals. In this study, we show for the first time the feasibility of designing synthetic steroids that can modulate different physiological processes, such as development, reproduction and ageing, in relation to ceDAF‐12. Our results not only confirm the conclusions derived from genetic studies linking these processes but also provide new chemical tools to selectively manipulate them, as we found that different compounds produce different phenotypic results. The structures of these compounds are much more diverse than those of endogenous hormones and analogues previously described by other researchers, allowing further development of the chemical modulation of the steroid endocrine system in C. elegans and related nematodes.

show abstract

The C. elegans lifespan assay toolkit

Cited by 112 publications

References 74 publications

Somatic increase of CCT8 mimics proteostasis of human pluripotent stem cells and extends C. elegans lifespan

Somatic increase of CCT8 mimics proteostasis of human pluripotent stem cells and extends C. elegans lifespan

Survival assays using Caenorhabditis elegans

Expanding the Repertoire of ceDAF‐12 Ligands for Modulation of the Steroid Endocrine System in C. Elegans

Contact Info

Product

Resources

About